| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,353 | A→G | 72.2% | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,353 | 0 | A | G | 72.2% | 54.1 / 21.3 | 40 | intergenic (‑86/+135) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/9); new base G (20/8); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGT > CP009273/2420220‑2420498 | gcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGtt > 2:375657/1‑149 (MQ=255) tGGAGAAGGCCAGAACCAGAGGGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGccc < 1:63149/143‑1 (MQ=255) gggggaAGGCCACAACCAGAGCGAGCGATCGCACCAGTTTTTTCGTAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTGACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg < 2:149447/145‑1 (MQ=255) gagaAGGCCAGAACCAGCAGGAGCGATAGCACCAGTTTTTTTATGTTGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCACGGCAAGATATCCCGCCCCGTTAGAc < 2:216994/149‑1 (MQ=255) gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACTGACAGTCGTTTTATAGGGAATATTTACAGGCGTGTGACACTTATCGTGCCTTCAAATTGCCCATCAAGGCAAGAGATCCCGCCCCGtaggac > 1:226152/1‑138 (MQ=255) gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCTTTATTTTTTCAACTGACAGTCCTTTTATAGGGAATCTTTACAGGTTTGTTACACTTATCGTGCCATACAATTGACGAACACGGCAAGATATCCCGCCACCTTAGAc > 1:112997/1‑143 (MQ=255) gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATACCGCCCCGTTAGACGtatta > 2:262451/1‑149 (MQ=255) gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTAGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGAc > 1:288987/1‑143 (MQ=255) gCCAGAACCAGAGAGAGCGATAGCACCAGGTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCCTAAAATTGACCATCAAGGCAAGATATCCCGcccccgtaggc > 1:143276/1‑136 (MQ=255) gAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtat < 1:368273/143‑1 (MQ=255) agaggcattgCACCAGTTTTTTCATAAGGTCCTCACCTGACAGCATTTTTATAGGGAATATTTACAGGTTTGTGATACTTATCGTGCCATAAAATTGACCATCAAGTCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa < 1:36117/134‑1 (MQ=255) agagGGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTCTAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATCTCCCGCCCCGTTAGACGTATTATTTTTCa < 1:11226/143‑1 (MQ=255) agagCGATAGCACCATTTTTTTCCTAAGGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTTACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa < 1:32276/143‑1 (MQ=255) agagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaaaa > 2:390315/1‑149 (MQ=255) cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGCCCATCAAGGTAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGc < 1:355664/143‑1 (MQ=255) aCCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCt > 1:118444/1‑143 (MQ=255) atgaTGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATg < 2:175003/146‑1 (MQ=255) aTAATGTCCTCAACTGTCAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATg < 2:247710/149‑1 (MQ=255) taaaCTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGcac < 1:180232/141‑1 (MQ=255) tGACAGTCTTTTTATAGGGAATATTTCCACGAAGACTATAGTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTCGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACgc > 1:175606/1‑142 (MQ=255) ttttATAGGGAATATTTACAGGTTTGTTAAACAACAAATGTCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaac > 1:273692/1‑143 (MQ=255) ttttATAGGGAATATTTACAGGGTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGAAGTATTATTTTTCATTAAAAATGCTGAAGGATGCATTTCAATGCACGACGATAGTGcaac > 1:96632/1‑143 (MQ=255) tttATAGGGAATTTTGCCAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTg < 2:354204/149‑1 (MQ=255) aaTATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCg > 1:198876/1‑143 (MQ=255) atTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatg > 2:355720/1‑149 (MQ=255) atTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGAGCACCatg > 2:355726/1‑149 (MQ=255) tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACTACGATAGTGCAACAAACCTGGCGTGCa > 1:373734/1‑143 (MQ=255) gTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCGTGATGATcct > 2:88851/1‑147 (MQ=255) gTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTCAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCCACAAACCTGACGCGCACCATGATGATCATTCCa > 2:406848/1‑149 (MQ=255) acacTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCa < 2:49826/149‑1 (MQ=255) cacTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAg < 2:340736/149‑1 (MQ=255) aactttctgTGCCATAAAATTGACCGTAAAGGTAGTATATCCGGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAg < 2:205529/141‑1 (MQ=255) tacagTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATc < 1:230714/139‑1 (MQ=255) cATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAATCCATCAGGTCCAg > 1:391886/1‑143 (MQ=255) ttGACCATCAAGGCAAAATATCCCGCCCCGTTCGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACACCCCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCa < 1:133443/143‑1 (MQ=255) cATCAAGGCAAGATATCCCGCCCCGTTAGACGTGTTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGAc < 1:194716/143‑1 (MQ=255) cATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCa > 2:124143/1‑149 (MQ=255) tCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCCCCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCGATCAGGTACAGCTTCCCAGCGACGTATCAGt > 2:66651/1‑149 (MQ=255) gCAAGATATCCCGCCCCGTTAGACGTATTATCTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGCTACAGCTTCCCCGCGACGTATCAg > 1:360384/1‑143 (MQ=255) cAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTAAccat > 2:156507/1‑147 (MQ=255) | GCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGT > CP009273/2420220‑2420498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |