| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,378,362 | A→C | 27.4% | S101R (AGC→CGC) | argR → | l‑arginine‑responsive arginine metabolism regulon transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,378,362 | 0 | A | C | 27.4% | 27.7 / 6.1 | 25 | S101R (AGC→CGC) | argR | l‑arginine‑responsive arginine metabolism regulon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (12/6); new base C (2/5); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
TGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGA > CP009273/3378223‑3378503 | tGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTGGTCGTGATTCATACCAGcc > 1:249927/1‑143 (MQ=255)tGCTGTACGTACACGAAATGCCAAAATGGAAATGGTTTACTGCCTGCCATCTGAACCGGGTGTACCAACCACCTCCAGTCCATCGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGcc > 1:249982/1‑143 (MQ=255) cTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTggcgcg > 2:250009/1‑149 (MQ=255) gtacACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCCGCCCTGGCGCGGCGCAg > 2:359846/1‑149 (MQ=255) tacACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACTAGGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGt > 2:405460/1‑149 (MQ=255) tacACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCATTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGt > 2:405537/1‑149 (MQ=255) ccAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAAtt < 1:19412/143‑1 (MQ=255) aCTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGc < 2:65146/149‑1 (MQ=255) gcctgccAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCCGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCaaa < 2:126368/149‑1 (MQ=255) gggTGTAGCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCCGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTa < 1:98058/143‑1 (MQ=255) ccaccTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCt < 2:369216/149‑1 (MQ=255) ccATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCCTAACCGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGGCTAACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGacac > 2:146373/1‑149 (MQ=255) ccATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGacac > 2:250393/1‑149 (MQ=255) cATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCTTACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACAcc > 2:123730/1‑149 (MQ=255) cTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCCGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTaccac < 2:173457/149‑1 (MQ=255) gggTGCGTGATACCGACTACATCGATGGAGTTGGCTTGATTCATACCAGCCCTGGCGCGGCGCAGTTCATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTGTGGGCACCATCGCTGGCGATGACACCATAttt < 1:95799/142‑1 (MQ=255) gCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGACTGCTGGACTCCGTGGGCAAAGCAGAAGGCATTCTGGGCACCATCGCTGACGATGACACCCTCTTTacca > 1:314565/1‑143 (MQ=255) ggATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTa > 2:422402/1‑149 (MQ=255) tatCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTGACACCCCTGCTacag > 2:356519/1‑146 (MQ=255) tACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGTTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTcac > 2:282340/1‑149 (MQ=255) gATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTcac > 1:340874/1‑143 (MQ=255) aGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCa < 1:310375/143‑1 (MQ=255) ttGTCGTCATTCATACCCGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGTTGGACTCACTGGGCAAAGCAGAAGGTATTCTGCGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCaaa < 1:167449/143‑1 (MQ=255) atctcttGATTCATAACATCCCCTGCGCGGCGCAGTTTTTTGTTCGCCTGCTGTACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCGGCTAACGGTTGCACAGTCaaa < 1:168461/137‑1 (MQ=255) tCATACCCGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGa < 2:355618/149‑1 (MQ=255) | TGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCCAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAGAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGGCGCAGTTAATTGCTCGCCTGCTGGACTCACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGA > CP009273/3378223‑3378503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |