| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,528,506 | A→C | 68.3% | V241G (GTA→GGA) | envZ ← | sensory histidine kinase in two‑component regulatory system with OmpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,528,506 | 0 | A | C | 68.3% | 26.6 / 5.3 | 21 | V241G (GTA→GGA) | envZ | sensory histidine kinase in two‑component regulatory system with OmpR |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (5/9); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAA > CP009273/3528381‑3528648 | gCTCAATGATGGCGTTGCACTCTTCTATATCTCTATTGATCGCTTCTGCCAGATAGCCATACTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCgg < 2:208770/149‑1 (MQ=255) tCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCACCAGCGt > 1:184539/1‑143 (MQ=255) gTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGtt < 2:130622/149‑1 (MQ=255) aCTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCTCCCGCCATCGGCAGCGTGCGGTCATCCGCCAGt > 1:148560/1‑143 (MQ=255) tCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCCGCAGCGGGCGGTCATCCGCCAGTTGCTTAACACCAgccgc > 2:374135/1‑149 (MQ=255) tGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTGAACACCAGCCGCCATATgg > 2:45069/1‑149 (MQ=255) gATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGTCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGt < 2:240448/149‑1 (MQ=255) aTCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCat > 1:310724/1‑143 (MQ=255) tCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCAACCGCAGCGTGAGGTCATCCGCCAGTTCCGTAacacaccccgcccaa > 1:327149/1‑132 (MQ=255) actCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAg < 2:134161/147‑1 (MQ=255) tGCCAGATAGCCTTCCTGCTCGCTCTTCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCAc < 2:307051/149‑1 (MQ=255) gCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTaaa < 1:171665/143‑1 (MQ=255) tcatcTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTCAAGGCCCGGGTAACGGAACGCACCTcc > 1:13465/1‑143 (MQ=255) ggCGAAGACGCGTCAGCGGCGTGCGCAAGTCGTGCCTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCa < 1:373837/143‑1 (MQ=255) cgcgTCAGCGGCGTGCGCAAGTCGTTACTTCCCCCCGCCATCAGCAGCGTGGGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAgcgg < 1:180363/143‑1 (MQ=255) gTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCCTAAGCAGCTTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGaat < 2:227064/149‑1 (MQ=255) gcgTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCCGCCGCCATATGGTTACAGGCCCGGGTAACGGAACGCACCTCCGAAGCGCCATACCCACGCAGCGGCGGCGGaata > 1:181249/1‑143 (MQ=255) gcgcAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCCCTACACTCACGCAGCGGCGGCGGAATAATCCCTTTAc < 2:211841/149‑1 (MQ=255) aGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCCTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAAcc < 2:169705/149‑1 (MQ=255) tGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCGGCGGCGGCGGAATAATCCCTTTACCAACCTGCaa > 2:349003/1‑149 (MQ=255) cTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTg > 1:130648/1‑143 (MQ=255) | GCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAA > CP009273/3528381‑3528648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |