Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,651,823 T→A 7.9% I33N (ATC→AAC)  gadE → gad regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,651,8230TA 7.9% 241.7 / 3.1 80I33N (ATC→AAC) gadEgad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (33/40);  new base A (3/4);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCT  >  CP009273/3651675‑3651971
                                                                                                                                                    |                                                                                                                                                    
aagaatGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGAt                                                                                                                                                      <  2:22933/146‑1 (MQ=255)
  taatGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTATCGTGACGAAAGATTCTTTTCTTTTAAAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCa                                                                                                                                                    >  2:3420/1‑149 (MQ=255)
   aatGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATAACGAAATGATAAAAATCAATTCCCTGTCAGAGAACaa                                                                                                                                                   >  2:115832/1‑149 (MQ=255)
            gATGGCATATTCGAAACGATTACGGCTAAGTAGCAAGTTATTATTTGTCTCATGACGAAAGATTCGTTTCTTGGACAGGGCTTTTGGCAGTGTAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAgg                                                                                                                                          <  2:414344/149‑1 (MQ=255)
            gATGACATATTCGAAACGATGACGGCTAAGGAGCAAGTGATGATTTTTCTCAGTACGAAAGAGTCGTTTCTTTGACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAAGGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAgg                                                                                                                                          <  2:414330/149‑1 (MQ=255)
             aTGAAATAGTCGAAACGATAACGGCTTATGATCAATTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTATAGGGCTTTTGGCAGTTGAAAGCTAATCACGAAAGGATAAAAATCAATTCCCTGTCAGAGATCaaaaaa                                                                                                                                               <  1:213332/143‑1 (MQ=255)
                  atatTCGAAACGATAACGGCTAAGGAGCAAGCTATGATTGTTCTCATGACGAAAGACTCTTTTCTTTTACAGGGCTTTTGGCAGTGGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATaa                                                                                                                                    <  2:34642/149‑1 (MQ=255)
                    atTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCTTGTCAGAGAACAAACAAGTAGGCAATAAAc                                                                                                                                  >  2:397374/1‑149 (MQ=255)
                    atTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCa                                                                                                                                        <  1:25183/143‑1 (MQ=255)
                     ttCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAAcc                                                                                                                                 >  2:36427/1‑149 (MQ=255)
                      tCGAAACGATAACGGCCAAGGAGCAAGTTATGATTTGTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAAccc                                                                                                                                <  2:13993/149‑1 (MQ=255)
                      gcGAGACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGGGTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAAt                                                                                                                                      >  1:359112/2‑143 (MQ=255)
                       cGAAACGATAACGGCTAAGGAGCAAGTTATGATTTGTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCt                                                                                                                               <  2:291543/149‑1 (MQ=255)
                        gAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCtt                                                                                                                              >  2:47980/1‑149 (MQ=255)
                            cGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAg                                                                                                                          <  2:220814/149‑1 (MQ=255)
                                    gCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGt                                                                                                                        >  1:35851/1‑143 (MQ=255)
                                      tAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTa                                                                                                                      >  1:244423/1‑143 (MQ=255)
                                       aaGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGTGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCCCAGATCTAAAAATTAGGCAATAAACCCTTCAAGGTTATCATTGa                                                                                                               <  2:320066/149‑1 (MQ=255)
                                         ggAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATa                                                                                                             <  2:163538/149‑1 (MQ=255)
                                         ggAGCAAGTTATGATTTTTCGCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCa                                                                                                                   <  1:302262/143‑1 (MQ=255)
                                          gAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCAt                                                                                                                  <  1:8547/143‑1 (MQ=255)
                                             caagaaaTGATTTATCTCATGACGAAAGAATCTTTTCTTTTACAGGGCTTTTCGCAGTTGAAAGATAAACACGAAAAAATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGa                                                                                                               <  1:4569/137‑1 (MQ=255)
                                             cAAGTTATGATTTTTCTACTGACCAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCAGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGa                                                                                                               <  1:240787/143‑1 (MQ=255)
                                             cAAGTTATCATTTTTCTCATGACGAAAGATTCTTGTATTTTACAGGGCTTTTCGCAGTTGAAAGATAATTAAGAAATGATAAAAATCAATTCCAAAAAAAAAAAAAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGa                                                                                                               <  1:145631/143‑1 (MQ=255)
                                              aaGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTAt                                                                                                        >  2:342141/1‑149 (MQ=255)
                                              aaGTGATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTAt                                                                                                        <  2:39264/149‑1 (MQ=255)
                                                gTTATGATTTTTCTCATGACGAACGATTCTTTTCTTTTACAGAGCTCTTTGCAGTTAAAAAATAATCACGAAATGATAAAAATCAATTCCCTAAAAAAAAAAAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATAc                                                                                                            <  1:189530/143‑1 (MQ=255)
                                                gTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATca                                                                                                      <  2:214151/149‑1 (MQ=255)
                                                   aTGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACaa                                                                                                   >  2:248423/1‑149 (MQ=255)
                                                   aTGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACaa                                                                                                   <  2:123146/149‑1 (MQ=255)
                                                    tGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCTAAAAAAAAAAAAAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTAt                                                                                                        <  1:188226/143‑1 (MQ=255)
                                                     gATTTTTCTCATGACGAAAGATTCTTTTCTTGTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATc                                                                                                 <  2:139381/149‑1 (MQ=255)
                                                          ttgtcATGACGAAAGATACTTTTCGTTTACAGGGCTTTTGGCGATTGAAACACAATCACGAAATGATAAAATTAATTTCGCTGTAATAAAAAAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAAt                                                                                                  <  1:43911/140‑1 (MQ=255)
                                                          ttCTCATTGCTAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCGATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAACCCCTTCGAGGTTATCATTGATACCTATCACAAt                                                                                                  <  1:388331/143‑1 (MQ=255)
                                                          ttCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAAt                                                                                                  <  1:202408/143‑1 (MQ=255)
                                                           tctcATGACTAAAGTTTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATc                                                                                                 <  1:351727/143‑1 (MQ=255)
                                                           tctcATGACGAAAGATTCGTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATc                                                                                                 <  1:168394/143‑1 (MQ=255)
                                                               aTGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCCTTGATACCTATCACAATCatat                                                                                             >  1:87108/1‑143 (MQ=255)
                                                               aTGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTtga                                                                                       >  2:75825/1‑149 (MQ=255)
                                                               aTGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTtga                                                                                       >  2:115862/1‑149 (MQ=255)
                                                                tGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGAAGTTGAAAGATAAGCAAGAAATGATAGAAATCAATTCCCTGTGAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATAACATTCttata                                                                                            >  1:241463/1‑142 (MQ=255)
                                                                         aTTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTAATACCTATCACAAACATATCCTTTATgaa                                                                                   >  1:128749/1‑143 (MQ=255)
                                                                            cttttcttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGAtt                                                                          <  2:127442/149‑1 (MQ=255)
                                                                             ttttcttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCCATTCCGGGTGAGTGATAAAATTAGCTTTCAATAAACCCTTCAAGGGTATCTTTGGAACCTAGCACAAGCATATCCTTGATGAAGAAGCGATTa                                                                         >  2:428604/1‑149 (MQ=255)
                                                                             ttttcttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATGAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTa                                                                         >  2:281218/1‑149 (MQ=255)
                                                                              tttcttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTAAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATCTCCTTGATGaagaagt                                                                              >  1:160412/1‑142 (MQ=255)
                                                                                 cttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATACAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAACCGAt                                                                           >  1:355546/1‑143 (MQ=255)
                                                                                 cttttACAGGGCTTTTGGCAGTTGAAAGATAATCAAGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCAATCACAATCATATCCTTGAGGAAGAAGCGAt                                                                           >  1:300870/1‑143 (MQ=255)
                                                                                  ttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGAtt                                                                          >  1:125672/1‑143 (MQ=255)
                                                                                  ttttACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAAttt                                                                    >  2:366469/1‑149 (MQ=255)
                                                                                     tACAGGGCTTTTGGCAGTTGAAAGATAATCACGCAATGATAAAAGGTAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTg                                                                 <  2:336045/149‑1 (MQ=255)
                                                                                     tACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGAGATTAAATTTCTg                                                                 <  2:295886/149‑1 (MQ=255)
                                                                                       cAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGga                                                               <  2:329574/149‑1 (MQ=255)
                                                                                        aGGGCTTTTGGCAGTTGAAAGATAAGCACGAAATGATAAAAATCAATTCCCTGTCAGAGAACAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTCATACCTATCACACTCATATCCTGGAGGAAGAAGCGATTAAATTGCTGgag                                                              >  2:259750/1‑149 (MQ=255)
                                                                                         gggCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAACttcc                                                                   <  1:160882/143‑3 (MQ=255)
                                                                                          ggCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCt                                                                  >  1:26090/1‑143 (MQ=255)
                                                                                           gCTTTTGTCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTg                                                                 <  1:211188/143‑1 (MQ=255)
                                                                                                tGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGaa                                                            <  1:258938/143‑1 (MQ=255)
                                                                                                tGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTCATCATTGATACCTATCAAAACCATATCCTTGACGAAGTAGCGATTAAATTTCTGGAGaa                                                            <  1:284711/143‑1 (MQ=255)
                                                                                                  gCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAAt                                                          <  1:382523/143‑1 (MQ=255)
                                                                                                        gAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCgaga                                              <  2:137558/149‑1 (MQ=255)
                                                                                                        gAAAGATAATCACGAAATGATAAAAATCAACTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATGACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCgaga                                              <  2:62608/149‑1 (MQ=255)
                                                                                                          aaGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCgagaga                                            >  2:275566/1‑149 (MQ=255)
                                                                                                                aTCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAattatt                                      <  2:257194/149‑1 (MQ=255)
                                                                                                                 tCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATACACCCTTCAAGGTTATCATTGATACCTATCACAATCATATACTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGGGAATTATTg                                     >  2:284483/1‑149 (MQ=255)
                                                                                                                  cACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAat                                          >  1:107050/1‑143 (MQ=255)
                                                                                                                   aCGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAatt                                         >  1:22846/1‑143 (MQ=255)
                                                                                                                      aaaTGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTg                                <  2:394942/149‑1 (MQ=255)
                                                                                                                       aaTGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTgg                               <  2:183510/149‑1 (MQ=255)
                                                                                                                         tGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGAGACCTATCACAATCATAGCCTTGATGAAGAAGCGATTAAAATTCTGGAGAAATTAGATGCCaagaggagagtagtt                                   >  1:319526/1‑133 (MQ=255)
                                                                                                                            tAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTg                                <  1:160941/143‑1 (MQ=255)
                                                                                                                             aaaaaTCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTgg                               >  1:219980/1‑143 (MQ=255)
                                                                                                                              aaaaTCAATTCCCTGTCAGAGATCAGAAAAGTAGGCAATAAACCCTTAAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTa                        >  2:243201/1‑149 (MQ=255)
                                                                                                                              aaaaTCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTCTTTTGGCACCTTa                        <  2:296943/149‑1 (MQ=255)
                                                                                                                               aaaTCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCa                             >  1:9644/1‑143 (MQ=255)
                                                                                                                                      ttCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTGCTGGAGAAATTAGATGCCGAGAGTATTATTGTTTTGGCACCTTATCACATCa                <  2:260886/149‑1 (MQ=255)
                                                                                                                                        cccTGTCAGAGATCAAAAAGGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGt              >  2:315270/1‑149 (MQ=255)
                                                                                                                                         ccTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTa             >  2:298897/1‑149 (MQ=255)
                                                                                                                                           tGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATc                 >  1:38358/1‑143 (MQ=255)
                                                                                                                                               agagATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAGTCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAgaa             <  1:85765/143‑3 (MQ=255)
                                                                                                                                                 agaTCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTaaaa     >  2:77410/1‑149 (MQ=255)
                                                                                                                                                    tCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCt  >  2:218561/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCT  >  CP009273/3651675‑3651971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑