| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,022,395 | C→T | 27.1% | G646D (GGC→GAC) | fadB ← | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,022,395 | 0 | C | T | 27.1% | 21.4 / 5.6 | 19 | G646D (GGC→GAC) | fadB | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (6/7); new base T (3/2); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTCTTCAACGGCGGCGTCTTCTTCTTTCTTCGGCTTACCT > CP009273/4022251‑4022541 | ccGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGGACGGCGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGGGGCGGTAATGCCTtc > 2:361530/1‑149 (MQ=255) gTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGTCTTCCTCCa > 2:233528/1‑149 (MQ=255) cGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGAcc > 2:417961/1‑149 (MQ=255) tttGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGGGCACCACTTCGTTGACCATCGGGat > 2:237380/1‑149 (MQ=255) gcaCCGAGGGGGTCCCGCCAGCGGAACGCCCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGat < 1:53880/141‑1 (MQ=255) aCCGAGGGTGTCCAGCCAGCGGAACGCGCGGCCGGGGAACGGTGTGAAGTCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGc < 2:405149/149‑1 (MQ=255) ccagcGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCgctca > 1:38369/1‑139 (MQ=255) aCGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATCATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTg > 2:39873/1‑149 (MQ=255) gCCCAGGCCGTAGACCAGCGCCATATCCCCCTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCggctggc < 1:160198/143‑1 (MQ=255) cccAGGCCTGAAACCAGCGCCACTTCCGCTTCCGC‑CGGAGTGGCGATAATGCCTCCCTCCAGATAGCGCACCACTTCTTTGACCATCGTTATCCTCATGCGGGCGGTAATCTCTTCTTCGCTGAAATCGCGCTTCggctggct < 1:1990/143‑1 (MQ=255) ggCCGTAGACCAGCGCCATCTCCGCTTCCGCGCGGA‑TGGACTTTCTGACTGCGTTTTCACAGCTCTCCCCTTCGTTGTCCATCGGTAGCTTCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACt < 1:272056/143‑1 (MQ=255) gTAGACCAGCGCCACAGCCGATTACGC‑CGGAGTGGCGATAATGTATTCCTCAATACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCcagca < 2:99624/149‑1 (MQ=255) cgcCCTGTCCGCTTCCGC‑CGGACTGGCGATAATGCCTTCCGCCAGACAGCGCACCACTTCGTCGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGt < 1:326798/143‑1 (MQ=255) gcCATATCCGCTTCCGC‑CGGAGTGGCGATAATGTCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATCATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTc < 1:204126/143‑1 (MQ=255) c‑cgGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTCTTCAACGGCGGCGtcttcttct < 2:227615/149‑1 (MQ=255) gCGATAATGCCTTCCTCCAGACAGCACACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTCGTCAACGGCGGCGTCTTCTTCTTTCTTCgg < 2:263781/149‑1 (MQ=255) gATAATGTCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCCGCAGGTCTTCAACGGCGGCGTCTTCttctttct > 1:79537/1‑143 (MQ=255) gATAATGTCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTCTTCAACGGCGGCGTCTTCttctttct > 1:269003/1‑143 (MQ=255) tGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATACGGGCGATACTCTCTGCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTCATCACCGGCGGCGACTTCTCCTTTCTTCGGCTTACCt > 2:338972/1‑149 (MQ=255) | CCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGC‑CGGAGTGGCGATAATGCCTTCCTCCAGACAGCGCACCACTTCGTTGACCATCGGGATCATCATGCGGGCGATAATCTCTTCTTCGCTGAAATCGCGCTTCGGCTGGCTCACTTCTGCCAGCAGGTCTTCAACGGCGGCGTCTTCTTCTTTCTTCGGCTTACCT > CP009273/4022251‑4022541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |