Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,217,123 A→G 19.0% Y487C (TAC→TGC)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,217,1230AG19.0% 54.2 / 7.8 31Y487C (TAC→TGC) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base A (13/12);  new base G (2/4);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

ATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATA  >  CP009273/2216987‑2217269
                                                                                                                                         |                                                                                                                                                  
aTTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTAt                                                                                                                                        >  1:313233/1‑149 (MQ=255)
  tCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTGCGGCCATTTTAtgt                                                                                                                                      <  1:316204/149‑1 (MQ=255)
    gTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGc                                                                                                                                    >  1:170925/1‑149 (MQ=255)
        tCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTCCCAGATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATg                                                                                                                                <  1:318201/149‑1 (MQ=255)
             cGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTc                                                                                                                           >  1:25415/1‑149 (MQ=255)
                                       aTTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAgg                                                                                                 >  1:29405/1‑149 (MQ=255)
                                          cTGGCGTTGGATATCGCTCTGCGGCGTAACGCCACCTAGTGGGATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGTTTACATATTGAAAAAAGGCGt                                                                                              <  1:330972/149‑1 (MQ=255)
                                          cTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGt                                                                                              <  2:313233/149‑1 (MQ=255)
                                                 tgtaaatagCTCGGCAGCGCAACGACACCGAGTGGTAAGAGCATTTGCGGGGGGAGAGCGACAGCGAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGAAAAAAAAAAAAAAAAAGGCGTGGAtgtg                                                                                       <  2:112073/141‑1 (MQ=255)
                                                     tatCGCTCTGCGGCGTAACGACACCGAGTGGTAGGCGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTGCGGCCATTTTATGTGCGATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATg                                                                                   <  1:19069/149‑1 (MQ=255)
                                                     catCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTGCGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATg                                                                                   <  1:56332/148‑1 (MQ=255)
                                                           tctGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTGCGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTaa                                                                             >  1:154906/1‑149 (MQ=255)
                                                              gcggcgTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGGTTACATAGTGAACAAAGACGTGGATGTGCATGCGTTCAAAg                                                                          >  2:168471/1‑149 (MQ=255)
                                                               cggcgTAACGCCACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACACAACTTTGTGGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGa                                                                         <  1:439609/149‑1 (MQ=255)
                                                                ggcgTAACGACACCGAGTTGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGaa                                                                        <  2:393809/149‑1 (MQ=255)
                                                                        gaagacgggTGGTATGAGCATTTACAGCCGGAGGACGACAGCCAGCTGGTGCACAAGCTCGATTGCGGCCCTCTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAGAGGGCGAGGATGGACATGCGGTAAAAGAACAGAtagt                                                                >  2:328626/9‑146 (MQ=255)
                                                                                   gTATGACCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTAc                                                     <  1:203532/149‑1 (MQ=255)
                                                                                    tATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTAAGTGCTATGTCTTCCATCAGGCTTACCTAGTGAAAGAACGCGCGGATGCGCATGCGCTGGAAGAGCAGATGCGGGAAGTGCTAca                                                    >  2:322939/1‑149 (MQ=255)
                                                                                               aCCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAgcgcggcg                                         <  1:168471/149‑1 (MQ=255)
                                                                                                   ccgGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCa                                     <  2:29405/149‑1 (MQ=255)
                                                                                                         aTCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTAccc                               >  1:400780/1‑149 (MQ=255)
                                                                                                          tCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCt                              >  1:401758/1‑149 (MQ=255)
                                                                                                            gACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGc                            >  1:353897/1‑149 (MQ=255)
                                                                                                                 ccagcTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGc                       >  1:38916/1‑149 (MQ=255)
                                                                                                                 agagcTTGTGCACCAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGCACAGCTACATGAACTGCGACAGCAGCGCGGCGCGCAGTACCCTGCCGAGc                       >  1:87587/3‑149 (MQ=255)
                                                                                                                  cagcTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCa                      >  2:57962/1‑149 (MQ=255)
                                                                                                                     cTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATaa                   >  1:388447/1‑149 (MQ=255)
                                                                                                                           gCACAAGCTCTATTACGGCCATTTTAGGTGCTATGTCTTCCATCAGGATTACAAAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCg              <  2:434799/149‑1 (MQ=255)
                                                                                                                                  ctctATTGCGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAttt       <  2:154906/149‑1 (MQ=255)
                                                                                                                                  ctctATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAttt       <  1:72764/149‑1 (MQ=255)
                                                                                                                                       ctACGGACATTTTATGTGCTATGTATTACATCATGACAACAAAGAGAAAAAAGGGGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGATCATAACGTCGGTCATTTGtata  <  2:159805/148‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
ATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATA  >  CP009273/2216987‑2217269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: