Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,420,394	G→T	intergenic (‑127/+94)	hisJ ← / ← argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,420,394	0	G	T	100.0%	155.2 / NA	50	intergenic (‑127/+94)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/19);  total (31/19)

CCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCT  >  CP009273/2420255‑2420531                                                                                                                                            |                                                                                                                                          ccAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTACTTTTCATTAAAAAATCTTa                                                                                                                                       >  2:662652/1‑144 (MQ=255)     ttttttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCAt                                                                                                                              <  1:418093/149‑1 (MQ=255)           cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAGGTATGCCTTTCAAt                                                                                                                        >  1:61960/1‑149 (MQ=255)                         cTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCCTAAAATTGACCATCAAGTCCACATATCCCGCCCCGTTCGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCCCGAtcgcg                                                                                                          >  1:470104/1‑145 (MQ=255)                          tGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGAt                                                                                                              >  2:356975/1‑144 (MQ=255)                              aGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCGTAAGTATGCATTTCAATGCACCACGAtgatg                                                                                                          >  2:131727/1‑140 (MQ=255)                                tCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGca                                                                                                        >  2:11974/1‑144 (MQ=255)                                gcTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGca                                                                                                        >  2:11989/2‑144 (MQ=255)                                  tttttATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCATCATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAcc                                                                                                 <  1:888416/149‑1 (MQ=255)                                             aaTATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTCTGACTTTCAATGCACCACGATAGTGCAACAAACCTGGAGt                                                                                           >  2:269928/1‑144 (MQ=255)                                             aaTATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCAcc                                                                                      >  1:419188/1‑149 (MQ=255)                                               tatTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGc                                                                                         >  2:321817/1‑144 (MQ=255)                                                atTTACAGTTTTGTTATACTTATCGTGCAATAAAAGTGACCATCAAGGCAACATATCCCGCCCCGTTATACGTATTATTTTTCATCAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatg                                                                                   <  1:913503/149‑1 (MQ=255)                                                  ttACAGGTTTGTTACACTTATCGTTCCATAAAATTGACCATCAAAGCAACATATCCCGCCCCGTTAGACATATTATTTTTCATTAAAAATTCTTAAGTATTCATTTCAATGCACAACGATAGTGCAACAAACCTTGGGTGCACCatgat                                                                                 >  1:450869/1‑149 (MQ=255)                                                        gTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAt                                                                           <  1:855017/149‑1 (MQ=255)                                                        gTTTGTTACACTTATCGTGCCATAAAATTAACCATCAAGACAACATGTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGAACCATGGTGATCAt                                                                           >  1:301319/1‑149 (MQ=255)                                                            gTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACAAATCTCGCCCCGTCGGACGTCTTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCa                                                                       >  1:223556/1‑149 (MQ=255)                                                             ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATCGTGCAACAAACATGGCGTGCACCATGATGATCAt                                                                           >  2:397674/1‑144 (MQ=255)                                                             ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAACCCTAGCGTGCCCCCTCATGATCac                                                                           >  2:738573/1‑143 (MQ=255)                                                              tACACTTATCGTGACATAAAAATGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATACTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATc                                                                     >  1:657629/1‑149 (MQ=255)                                                                cccTTATCGTGCGATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTcc                                                                        <  2:89450/142‑1 (MQ=255)                                                                cacTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTcc                                                                        <  2:531487/144‑1 (MQ=255)                                                                  ctcaTCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGt                                                                 >  1:102384/4‑149 (MQ=255)                                                                  cTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGt                                                                 >  1:703068/1‑149 (MQ=255)                                                                   ttATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCCTGATGATCATTCCATc                                                                     >  2:344635/1‑144 (MQ=255)                                                                          gCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTc                                                         <  1:69087/149‑1 (MQ=255)                                                                           ccATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACTTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAg                                                             <  2:788067/144‑1 (MQ=255)                                                                           ccATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTcc                                                        <  1:624316/149‑1 (MQ=255)                                                                             aTAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCa                                                      >  1:607822/1‑149 (MQ=255)                                                                              tAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAg                                                     >  1:811124/1‑149 (MQ=255)                                                                               aaaaTTGACCATCAAGTCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTc                                                         <  2:390956/144‑1 (MQ=255)                                                                                aaaTTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATCCCATCAGGTACAGCTTcc                                                        >  2:563869/1‑144 (MQ=255)                                                                                  aTTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGAc                                                 <  1:836231/149‑1 (MQ=255)                                                                                  aTTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGAc                                                 <  1:769077/149‑1 (MQ=255)                                                                                   ttGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACg                                                >  1:353083/1‑149 (MQ=255)                                                                                   tggACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCTTTTTTTTTTCATAAGTATGCATGTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCCCCCTGCTGATCATTCCATCACGTACAGCCTCCCAg                                                     >  2:149143/3‑144 (MQ=255)                                                                                     taCCATCAAGTCAACATATCCCTCCCCGTTATACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTa                                              <  1:380945/148‑1 (MQ=255)                                                                                          tCAATGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt                                         <  1:397156/149‑1 (MQ=255)                                                                                           cAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACTAACCTGTCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTAt                                             <  2:547839/144‑1 (MQ=255)                                                                                               gCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACCAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt                                         >  2:632628/1‑144 (MQ=255)                                                                                                        cccGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTa                           >  1:756820/1‑149 (MQ=255)                                                                                                         ccGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCCCCGTAGAc                               >  2:1340/1‑144 (MQ=255)                                                                                                         ccGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTAATGCTTCCCAGGGACGTATCAGTCACCGTAGACATTaa                          >  1:156184/1‑149 (MQ=255)                                                                                                            ccccGTTAGACGTATTATGTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGt                       <  1:107348/149‑1 (MQ=255)                                                                                                               cGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTaaa                         <  2:203913/144‑1 (MQ=255)                                                                                                                         attattTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACttttt          <  1:867083/149‑1 (MQ=255)                                                                                                                         attattTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATAAGGTACTTCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACttttt          <  1:725189/149‑1 (MQ=255)                                                                                                                           tattTTTCATTAAAAATTCTTAAGTACCCACGCCCAGGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATACCATCAGGTACAGCTTCCCAGCGACGGATCAGTCACCGTAGACATTAAAGTCGAAATCCtt             >  2:386437/1‑144 (MQ=255)                                                                                                                             tttttCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACtttt           >  2:471977/1‑144 (MQ=255)                                                                                                                                 tCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCt  >  1:613414/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                          CCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCT  >  CP009273/2420255‑2420531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑