Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,138,538 T→G E429A (GAA→GCA)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,138,5380TG99.0% 331.9 / NA 100E429A (GAA→GCA) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (44/55);  minor base C (1/0);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAA  >  CP009273/1138395‑1138680
                                                                                                                                               |                                                                                                                                              
cGTTTGGCACAATTCCACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGGAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCg                                                                                                                                           <  2:1854498/149‑1 (MQ=255)
  tttGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGAt                                                                                                                                         <  2:1596409/149‑1 (MQ=255)
   ttGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGcttctgctt                                                                                                                                             <  1:489691/144‑1 (MQ=255)
       cacaATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGAt                                                                                                                                         >  1:1132557/1‑144 (MQ=255)
         caATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTTGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGc                                                                                                                                  <  2:276465/149‑1 (MQ=255)
           aTTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAg                                                                                                                                >  2:1072768/1‑149 (MQ=255)
            ttACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGGGTTCTCTTTCAGCGCTTCTGCTTGGATCCGACGCAGa                                                                                                                               >  2:1586593/1‑149 (MQ=255)
              acacacCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGc                                                                                                                                  <  1:1549786/144‑1 (MQ=255)
               cacaGCGCACACCGTCCTGACGAGTTTCAATGGCATTCACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTTGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGCCGCa                                                                                                                                 <  1:1492231/144‑1 (MQ=255)
                 caGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATaga                                                                                                                          <  2:1583823/149‑1 (MQ=255)
                 caGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATaga                                                                                                                          <  2:1165514/149‑1 (MQ=255)
                     gcACACCTTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCCGCGCTTCTGCTTCGATCAGACGCAGCATag                                                                                                                           >  1:676570/1‑144 (MQ=255)
                     gcACACCGTCCTGACGAGTTTCAATGTCATTTACCGCAGAACGTCTTTCATTCTGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGTTGTTCTCTTTCAGCGCTTCTGTTTCGATCAGACGCAGAATAGAGAGc                                                                                                                      <  2:1115055/149‑1 (MQ=255)
                     gcACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGc                                                                                                                      <  2:487346/149‑1 (MQ=255)
                     gcACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGGTTTTCATTCAGCAGGTAAGAAGCGATTAGCCCAGGAACAATGGCCTGAACTTCCTGCGTGTTCTCTTTCAGCGCTTCTGCTTCGAACAGACGCAGAATag                                                                                                                           >  1:114228/1‑144 (MQ=255)
                      caccCCGTCCTGACGAGTTTCAATGGCATTTCCCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATaga                                                                                                                          <  1:807881/144‑1 (MQ=255)
                      cacaCCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCCCAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGAGCAGACGCAGAAtggc                                                                                                                          >  1:746535/1‑141 (MQ=255)
                      cacaCCGTCCGGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAACGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCg                                                                                                                     <  2:914789/149‑1 (MQ=255)
                       acagCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATagag                                                                                                                         <  1:1069383/144‑1 (MQ=255)
                        cacCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGGTTCGGTCAGACGCAGAATAGAGAGCGAc                                                                                                                   <  2:1097792/149‑1 (MQ=255)
                        cacCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATagaga                                                                                                                        <  1:223669/144‑1 (MQ=255)
                        cacCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATagaga                                                                                                                        <  1:1327421/144‑1 (MQ=255)
                         gccGTCCTGACGAGTTTTAATGGCATTTACCGAAGTACTCTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATagagag                                                                                                                       <  1:1258380/143‑1 (MQ=255)
                             tccgggcGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGa                                                                                                              <  2:591848/143‑1 (MQ=255)
                              ccTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCCTTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACa                                                                                                                  <  1:779296/144‑1 (MQ=255)
                              ccTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACa                                                                                                                  <  1:795962/144‑1 (MQ=255)
                                tGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCCTTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTc                                                                                                           <  2:1270419/149‑1 (MQ=255)
                                   cgcgTTTCAATGGCATTTACCGCAGAACTTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGtt                                                                                                        <  2:1710927/146‑1 (MQ=255)
                                   cGAGTTTCGATGGCAGTTACCGCAGAACTGTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGTAACAATGGCGCGAACTCCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGtt                                                                                                        <  2:1597105/149‑1 (MQ=255)
                                       tttCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACCGGAACAATGGAGTGAACTTCCTGGGTGTTCTCTTTCCGCGCTTCTGCTTCGTTCAGACGCGGAATCGAGAGCGACAGGGATTCGt                                                                                                         >  1:934724/1‑144 (MQ=255)
                                       tttCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACCGCGATTCGTTGTca                                                                                                    >  2:1189756/1‑149 (MQ=255)
                                        ttaaagtgCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTGCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGtt                                                                                                        <  1:851751/137‑1 (MQ=255)
                                        ttaaaTGGCATTTACCGAAGAACGTGTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTcac                                                                                                   <  2:1670530/146‑1 (MQ=255)
                                        ttCAATGGCATTTCCCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCCATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTATTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGATAGCGACAGCGATTCGtt                                                                                                        <  1:1351806/144‑1 (MQ=255)
                                         tCAATGGCATTTACCTCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTg                                                                                                       <  1:739502/144‑1 (MQ=255)
                                            aTGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTCGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTca                                                                                                    <  1:1351085/144‑1 (MQ=255)
                                             tGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGAGATTACTAGCcacgcacg                                                                                              >  2:1729877/1‑149 (MQ=255)
                                             tGGCATTTACCGCAGAACGTTTTTAATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTGAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTAGTGGTgac                                                                                                   >  1:619031/1‑144 (MQ=255)
                                               gCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTcacgg                                                                                                 >  1:178328/1‑143 (MQ=255)
                                               gCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGt                                                                                            <  2:356489/149‑1 (MQ=255)
                                                 aTTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTcacgcac                                                                                               >  1:858467/1‑144 (MQ=255)
                                                     aCCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGt                                                                                      <  2:1176294/149‑1 (MQ=255)
                                                       cGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGcc                                                                                         <  1:1710480/144‑1 (MQ=255)
                                                        gCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTAcc                                                                                   >  2:1146441/1‑149 (MQ=255)
                                                        gCAGAACGGTTTTCATTCAGCAGTTAACAAGCGATTGGCTCAGTAACAATCCCGGTAACTTCCTGGGTGTTCTCTCTCAGCGCTTCTGCTTCGATCAGACGCAGACTAGACAGCGACAGCGATTCGTTGTCACGCACGGTGCCa                                                                                        <  1:1370227/144‑1 (MQ=255)
                                                        gCAGAACGGTTTTCATGCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACATCGATTCGTTGTCACGCACGGTGccg                                                                                        <  1:719281/144‑2 (MQ=255)
                                                         cAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCa                                                                                  <  2:191665/149‑1 (MQ=255)
                                                            taCGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACGGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGaa                                                                               <  2:814626/148‑1 (MQ=255)
                                                            aaCGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGaa                                                                               <  2:133387/149‑1 (MQ=255)
                                                              cGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCa                                                                                  <  1:303704/144‑1 (MQ=255)
                                                                tttttCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCCCGGTGCCAGTACCAGa                                                                                >  1:778410/1‑144 (MQ=255)
                                                                 ttttCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGaa                                                                               <  1:1411459/144‑1 (MQ=255)
                                                                  tttCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGaac                                                                              >  1:867752/1‑144 (MQ=255)
                                                                  tttCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGaac                                                                              >  1:652388/1‑144 (MQ=255)
                                                                    tcattcaGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGACCAACGCgg                                                                       >  2:252526/1‑149 (MQ=255)
                                                                        tcaGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAAcgcg                                                                        <  1:843402/144‑1 (MQ=255)
                                                                          agcagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCGGTACCAGAACAACGCGGACAAAc                                                                 >  2:469773/1‑149 (MQ=255)
                                                                          agcagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAAc                                                                 >  2:297098/1‑149 (MQ=255)
                                                                           gcagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACg                                                                >  2:1971595/1‑149 (MQ=255)
                                                                            cagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGt                                                               <  2:1496953/149‑1 (MQ=255)
                                                                             agGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCATGGGGTTTGCCAGTACCAGAACAACGCGGACAAACGtg                                                              <  2:971166/149‑1 (MQ=255)
                                                                              ggTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                             <  2:195017/149‑1 (MQ=255)
                                                                              ggTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGATTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTCCCAGAACAACGCggcacaa                                                                  >  1:940324/1‑139 (MQ=255)
                                                                                   gaagCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAGAGCGGACACACGggatgagt                                                        >  2:613400/1‑147 (MQ=255)
                                                                                    aagCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGATTCGATCAGAGGCAGAATATGAAGCGACAGCGATTCGTTGTCACGCACGGGGCCAGTACCAGAACAACGCGGACAAACGTGATGAcgg                                                       >  2:1948470/1‑147 (MQ=255)
                                                                                       cGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                    >  2:1362670/1‑149 (MQ=255)
                                                                                        gATTGACACATGAAGAAGGGGGTTAACTTCCTGGGGGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACACCGCGGACAACCGTGATGACt                                                        >  1:880796/1‑144 (MQ=255)
                                                                                           tGGGACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCCGACAAACGTGATGACTGGATTCAc                                                <  2:1004249/149‑1 (MQ=255)
                                                                                           tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCATCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGa                                                     <  1:1193493/144‑1 (MQ=255)
                                                                                           tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGa                                                     <  1:1820112/144‑1 (MQ=255)
                                                                                             gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAtt                                                   >  1:1751244/1‑144 (MQ=255)
                                                                                             gCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAtt                                                   >  1:329404/1‑144 (MQ=255)
                                                                                               acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGGGGTTCTCTAATTATCCGACGCAGAATGGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACATGCTGACTGGGTTCACCCAg                                            >  2:474442/1‑149 (MQ=255)
                                                                                               acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAg                                            <  2:733082/149‑1 (MQ=255)
                                                                                               acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGAGCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCa                                                 >  1:1834504/1‑144 (MQ=255)
                                                                                                 aGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTg                                          >  2:1229589/1‑149 (MQ=255)
                                                                                                   gAACAATGGCGTGAACTTACTGGGGGTTCTCTTTCAGCGATAGTGGTTCGATCAGGCGCAGGAGTGTGTGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAATGCGGACAAACGTGATGACTGGATCCACCCa                                             >  1:391218/1‑144 (MQ=255)
                                                                                                     aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGt                                           <  1:1844773/144‑1 (MQ=255)
                                                                                                            cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACCGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACCGGATTCACCCAGCAATggga                                    >  1:9326/1‑143 (MQ=255)
                                                                                                            cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGc                                    >  1:157765/1‑144 (MQ=255)
                                                                                                            cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                               >  2:925727/1‑149 (MQ=255)
                                                                                                              tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGcgc                                  >  1:1527516/1‑142 (MQ=255)
                                                                                                                   ttCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc                        >  2:358413/1‑149 (MQ=255)
                                                                                                                    tCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACGCAGTGATGGGCTCAGGCGCTGACg                       <  2:1005021/149‑1 (MQ=255)
                                                                                                                    tCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACg                       <  2:194646/149‑1 (MQ=255)
                                                                                                                    tCCTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAAAAAGGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGTTCAGGCGCTGACg                       <  2:946102/149‑1 (MQ=255)
                                                                                                                     ccTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTTGTTGTCACGCACGGTGCCAGTACAAGAACAACGAGGACAAACGTGATGACTGGATTGACCCAGTGATGGGCTCAGGCGCtatcgg                      >  2:395507/1‑144 (MQ=255)
                                                                                                                      cTGGGTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTg                          >  1:1122689/1‑144 (MQ=255)
                                                                                                                         ggTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGACTAGAGAGCGACAGCGATTGGTTGTCACGCACGGTGCCAGGACCAGCACAACGCGGACAAACGTGATGACGGGAGTCGCCCGGGGATGGGGTCGGGCGCTGACg                       >  1:807131/1‑144 (MQ=255)
                                                                                                                         ggTGTTCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACg                       <  1:570863/144‑1 (MQ=255)
                                                                                                                             ttCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTc              <  2:770015/149‑1 (MQ=255)
                                                                                                                             ttCTCTTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTc              >  2:852547/1‑149 (MQ=255)
                                                                                                                               ctctTTCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACCGCGATTCGTTGTCACGCACGGTGCCAGTACCCGAACAACGCGGACAAACCTGATGACTGGATTCACCACGCGATTGGCTCAGGCGCTGACGGGACAt                 >  1:1193400/1‑144 (MQ=255)
                                                                                                                                  tttCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACAAGAACAACGCGGACAAACGTGATGCCTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagca         >  2:40864/1‑149 (MQ=255)
                                                                                                                                   ttCAGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc             >  1:569297/1‑144 (MQ=255)
                                                                                                                                    tCAGCGCTTCTGCTTCGATCAGCCGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAgg       <  2:1166254/149‑1 (MQ=255)
                                                                                                                                      aGCGCTTCTGCTTCGATCAGACGCAGAATAGAGGGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCGGGcc     >  2:1267811/1‑149 (MQ=255)
                                                                                                                                      aGCGCTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc          >  1:1095704/1‑144 (MQ=255)
                                                                                                                                         gcTTCTGCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCaaa  <  2:1017870/149‑1 (MQ=255)
                                                                                                                                           ttctgcttcGATCAGACGCAGACTAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGcc     >  1:774388/1‑144 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAA  >  CP009273/1138395‑1138680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑