Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	1,716,881	(GAT)4→3	7.0%	coding (504‑506/2013 nt)	ydhK →	putative efflux protein (PET) component of YdhJK efflux pump

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	1716873 =	58 (0.830)	4 (0.060)	4/254	NT	7.0%	coding (496/2013 nt)	ydhK	putative efflux protein (PET) component of YdhJK efflux pump
	?	CP009273	= 1716869	53 (0.820)					coding (492/2013 nt)	ydhK	putative efflux protein (PET) component of YdhJK efflux pump

TCTGCCCAATCACCCCTTCATGTGCTGCACGAATGGCATCGGTTGTTTCAGGCTGCCAGAGTAAACTGGCATGTTCCAGTAATCGGGCGTGCATGTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATCATCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/1717026‑1716873 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atcatcatcatGCCGCCGCACAAAATACCGACTATTACCTCGCAAACGCGCGCCTGAGCGATATCCCACAGCTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGATGATGGCAGCCGTGTAGCCCGCCAGTTGAAATGCATACGCGACG  <  CP009273/1716869‑1716728                                                                                                                                                                                                                                                                                                          TCTGCCCAATCACCCCTTCATGTGCTGCACGAATGGCATCGGTTGTTTCAGGCTGCCAGAGTAAACTGGCATGTTCCAGTAATCGGGCGTGCATGTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATC                                                                                                                                                     >  2:1201716/1‑149  CTGCCCAATCACCCCTTCATGTGCTGCACGGATGGCATCGGTTGTTTCAGGCTGCCAGAGTAAACTGGCATGTTCCAGTAATCGGGCGTGCATGTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATCA                                                                                                                                                    <  2:529699/149‑1    GCCCAATCACCCCGTCATGGGCTGCACGAATGGCATCGGTTGTTTCAGGCTGCCAGAGTAAACTGGCATGTTCCAGTAATCGGGCGTGCATGTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATCATC                                                                                                                                                  <  2:1229561/149‑1     CCCAATCACCCCTTCATGTGCTGCACGAATGGCATCGGTTGTTTCAGGCTGCCAGAGTAAACTGGCATGTTCCAGTAATCGGGCGTGCATGTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCACCATCA                                                                                                                                                 >  2:459906/1‑149                                                   GGCTGCGAGATTAAATGGGCAGGTTCCAGTAATCGGGCCTGCATTTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATCATCATGCCGCCGCACAAAATACCGACTATTACCTCGCAAACGCGCGCCTG                                                                                                   <  2:572936/149‑1                                                                                                               AAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATCATCATGCCGCCGCACAAAATACCGACTATTACCTCGCAAACGCGCGCCTGAGCGATATCCCCCAGCTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGATGATGGC                                       >  1:461924/1‑149                                                                                                                                        gCGGCAGCATCATCATCATGCCGCCTCAAAAAATACCGGCCATCACCTCGCAAACTCGCTCCTGAGCGATATCCCACAGCTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGATGATGGCAGCCCTGTAGCCCGCCAGTTGAAAT              <  2:865182/148‑1                                                                                                                                          GGCAGGATCATCATCATGCCGCCGCACAAAATACCGACTATTACCTCGCAAACGCGCGCCTGAGCGATATCCCACAGCTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGATGATGGCAGCCGTGTAGCCCGCCAGTTGAAATGC            <  1:355174/149‑1                                                                                                                                                  CATCATCATGCCGCCGCACAAAATACCGACTATTACCTCGCCACTGCTTGCCTAAGCAATAACCGGCAACTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGATGATGGCAGCAGTGTAGCCCGCCAGTTGAAATGCATACGCGA    >  1:1220064/1‑149                                                                                                                                                    TCATCATGCCGCCGCACAAAATACCGACTATTACCTCGCAAACGCGCGCCTGAGCGATATCCCACAGCTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGCTGATGGCAGCCGTGTAGCCCGCCAGTTGAAATGGATCCGCGACG  >  1:110586/1‑149                                                                                                                                                                                                                                                                                                          TCTGCCCAATCACCCCTTCATGTGCTGCACGAATGGCATCGGTTGTTTCAGGCTGCCAGAGTAAACTGGCATGTTCCAGTAATCGGGCGTGCATGTTTTTCAATGCGGTTAAAAGGGCAGTAGCATCGGAACTGCTCGGCAGGATCATCATCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/1717026‑1716873 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atcatcatcatGCCGCCGCACAAAATACCGACTATTACCTCGCAAACGCGCGCCTGAGCGATATCCCACAGCTGGCTGGCCTCAGTAATATTAACCATCGGAAAGGCGATGATGGCAGCCGTGTAGCCCGCCAGTTGAAATGCATACGCGACG  <  CP009273/1716869‑1716728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.