Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 796,346 T→C 6.9% D44D (GAT→GAC ybhH → putative PrpF family isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273796,3460TC 6.9% 215.5 / 4.0 83D44D (GAT→GACybhHputative PrpF family isomerase
Reads supporting (aligned to +/- strand):  ref base T (35/42);  new base C (4/2);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

AAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAA  >  CP009273/796198‑796489
                                                                                                                                                    |                                                                                                                                               
aaaTTGAGGGCATTATTACGAAAAAAATACCCGGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGAt                                                                                                                                                 <  2:480919/149‑1 (MQ=255)
  aTTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGCGCGCGTTCCTGTTAGCGGAACATTGACCCGAAGATCAAACGCAGCGCGATGAAATATTGATGGCAATTATGGGTTCCGGTAACGATCt                                                                                                                                               <  1:803113/149‑1 (MQ=255)
    tGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTgg                                                                                                                                             >  2:34826/1‑149 (MQ=255)
     gAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGa                                                                                                                                            <  1:456132/149‑1 (MQ=255)
      aGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGACCTGGaa                                                                                                                                           >  2:391738/1‑149 (MQ=255)
       gggCATTATTATGAAGAAAGTACCCTGCGTGAGGATGGGAGGGGGAACCTCGAGGGGCGCGTGCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaaa                                                                                                                                          <  2:106926/149‑1 (MQ=255)
       gggCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaaa                                                                                                                                          <  2:160761/149‑1 (MQ=255)
            ttattaTGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAc                                                                                                                                     <  1:1161121/149‑1 (MQ=255)
             tattatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACg                                                                                                                                    <  1:34826/149‑1 (MQ=255)
              attatGAAAAAAATCCCCTGCGTGATGATGCGAGTTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCCAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACgg                                                                                                                                   <  1:979498/149‑1 (MQ=255)
                tatGAAAAAAATACCCTGCGTGATCATGCGAGGTGGATCCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTa                                                                                                                                 <  2:679222/149‑1 (MQ=255)
                     aaaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTACCGATCTGGAAATTGACGGTATTggg                                                                                                                            >  2:80547/1‑148 (MQ=255)
                        aaaGACCGTGCGTGATGATGCGAGGTGGAACCCCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTggcggc                                                                                                                         <  1:460779/149‑1 (MQ=255)
                        aaaGACCCTGCGTTATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTCCCCGAAGATCAAACGCAGCGCGATAAAATATGGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTggcggc                                                                                                                         <  1:418977/149‑1 (MQ=255)
                          aTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGcggcgg                                                                                                                       <  1:994772/149‑1 (MQ=255)
                           tACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGt                                                                                                                      <  1:923015/149‑1 (MQ=255)
                                  cGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACAGTTACCCGAAGATCAAACGCAGCGGGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCAc                                                                                                               >  2:1145873/1‑149 (MQ=255)
                                      ttgatgCGAGGTGGAACCTCGGGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAc                                                                                                           <  1:704432/148‑1 (MQ=255)
                                      atgatgCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAc                                                                                                           <  2:787519/149‑1 (MQ=255)
                                       cccctcgaggttccaCCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAAGGCAGCGCGGTAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGGGGTAGTGCACTGAcc                                                                                                          >  1:572945/15‑149 (MQ=255)
                                       cccctcgaggttccaCCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAcc                                                                                                          >  1:568392/15‑149 (MQ=255)
                                           gCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTa                                                                                                      <  1:922058/149‑1 (MQ=255)
                                                gTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTc                                                                                                 >  1:426329/1‑149 (MQ=255)
                                                gTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTc                                                                                                 >  2:1042741/1‑149 (MQ=255)
                                                gTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGGACAGTc                                                                                                 >  2:659408/1‑149 (MQ=255)
                                                gTGGAACCTCGAAGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGGCCAGTACAGta                                                                                                 >  1:893063/1‑148 (MQ=255)
                                                 tggagacTCGCGGGGCGCGTTCCTGTTAGCGGAACATTTAGCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCg                                                                                                <  2:401836/143‑1 (MQ=255)
                                                       cTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAACCGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGTGAATCCACTGACCAGTAAAGTCGCCatta                                                                                          >  2:409358/1‑149 (MQ=255)
                                                       cTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCatta                                                                                          >  2:1189578/1‑149 (MQ=255)
                                                       cTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCatta                                                                                          >  1:635057/1‑149 (MQ=255)
                                                        tCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGAAAGTGTGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCattat                                                                                         >  1:1094572/1‑149 (MQ=255)
                                                              ggCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCg                                                                                   <  2:739364/149‑1 (MQ=255)
                                                               gcgcGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGt                                                                                  <  2:439990/149‑1 (MQ=255)
                                                                  cgTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGGTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTcc                                                                               >  2:947975/1‑149 (MQ=255)
                                                                    ttCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAg                                                                             <  2:856902/149‑1 (MQ=255)
                                                                     tCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATGGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGc                                                                            <  1:748269/149‑1 (MQ=255)
                                                                      ccTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCg                                                                           >  1:1069037/1‑149 (MQ=255)
                                                                      ccTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCg                                                                           <  1:211121/149‑1 (MQ=255)
                                                                         gTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTAGTAGCCGTTCCAGCGATc                                                                        >  1:418800/1‑149 (MQ=255)
                                                                          ttAGCGGAACATTTACCCGAAGATCAAAAGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATcc                                                                       <  1:557242/149‑1 (MQ=255)
                                                                           tAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGACGCCATTATTAGCCGTTCCCGCGATGcg                                                                      >  2:833011/1‑149 (MQ=255)
                                                                            aGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGCTCTGGAAATTGACGGTATTGGCGGCGGTCATCCACTGACCGGTAAAGTCGACATTATTAGCCGTTCCAGCGATCcgc                                                                     >  2:1222541/1‑149 (MQ=255)
                                                                            aGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCcgc                                                                     <  1:997251/149‑1 (MQ=255)
                                                                            aGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAACGATCcgc                                                                     <  2:695833/149‑1 (MQ=255)
                                                                            aGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCACTATTAGCCGCTCCAGCGACCcgc                                                                     >  2:98716/1‑149 (MQ=255)
                                                                              cgtaaaaTTTACCCTAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTCTTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGt                                                                   <  1:609098/143‑1 (MQ=255)
                                                                              cGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGt                                                                   >  1:540319/1‑149 (MQ=255)
                                                                                   cATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGGGCTGa                                                              >  1:155961/1‑149 (MQ=255)
                                                                                   cATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAAGGTCTTGGCGGCGGTAAACCACGGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa                                                              >  1:1158181/1‑149 (MQ=255)
                                                                                      ttccccGAAGATCAAACGCAGCGCGATAAAATATTGAGGGCAATTATGGGTTGCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGCTGt                                                           <  2:415867/146‑1 (MQ=255)
                                                                                      ttACCCGCAGATCAAACGCAGCGCGATAAAATATTGATGGCAAGTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGt                                                           <  2:1063436/149‑1 (MQ=255)
                                                                                      ttACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGt                                                           >  1:111980/1‑149 (MQ=255)
                                                                                      ttACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGt                                                           >  1:677504/1‑149 (MQ=255)
                                                                                      ttACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCGGATGt                                                           >  2:1229716/1‑149 (MQ=255)
                                                                                      ttACCCGAAGATCAAACGCAACCCGATCAAATAGTTATGGCAATTATGGGTTCCGGTAACGACCGAGAAATTGACGGTATTGGCGGCGGTAACCCACTGACCAGGAAAGTCGGCATTACTAGCCGTACCAGCGAACCGCGTGccgcggg                                                           >  2:322169/1‑143 (MQ=255)
                                                                                         cccGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCgg                                                        >  1:439109/1‑148 (MQ=255)
                                                                                         cccGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGACCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa                                                        >  2:1126599/1‑149 (MQ=255)
                                                                                         cccGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGACCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa                                                        >  2:1126761/1‑149 (MQ=255)
                                                                                          ccGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGAt                                                       <  1:142817/149‑1 (MQ=255)
                                                                                          ccGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGAt                                                       <  2:923430/149‑1 (MQ=255)
                                                                                                aTCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTAACTg                                                 >  1:322935/1‑149 (MQ=255)
                                                                                                 tCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt                                                <  2:1086929/149‑1 (MQ=255)
                                                                                                  cAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAACTTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt                                               >  1:1221031/1‑149 (MQ=255)
                                                                                                  cAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt                                               >  1:450704/1‑149 (MQ=255)
                                                                                                  cAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATATCGATTATCTGtt                                               >  2:1173568/1‑149 (MQ=255)
                                                                                                    aaCGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTg                                             <  1:664993/149‑1 (MQ=255)
                                                                                                     aCGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgc                                            <  1:965882/149‑1 (MQ=255)
                                                                                                     aCGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgc                                            <  2:1088333/149‑1 (MQ=255)
                                                                                                         aGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAg                                        <  1:196799/149‑1 (MQ=255)
                                                                                                                  aaaTATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGtt                               >  2:364887/1‑149 (MQ=255)
                                                                                                                      atTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATg                           >  1:591650/1‑149 (MQ=255)
                                                                                                                      atTGATGGCAATTATGGGTTCCGGAAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATg                           >  1:923920/1‑149 (MQ=255)
                                                                                                                           tGGCAATGATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa                      <  1:531548/149‑1 (MQ=255)
                                                                                                                            ggCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGGGCAAc                     >  1:244292/1‑149 (MQ=255)
                                                                                                                            ggCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc                     >  2:990785/1‑149 (MQ=255)
                                                                                                                                 ttATGGGTCCCCGTAACGATCTGGAAATTCACGGTATTGGCGGCGGTAATCCACGGACCAGTAAAGTCGCCATTATTAGCCGTTCCACCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc                <  2:996798/149‑1 (MQ=255)
                                                                                                                                  taggggTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCCGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCg               <  2:1042600/146‑1 (MQ=255)
                                                                                                                                  taggggTTCCGGTAACGATCTGGAAATGTACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGAGCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCg               <  2:961343/146‑1 (MQ=255)
                                                                                                                                  tATGGGTTCCGGTAACGATCTGGAAATGGACGTTATTGGAGGCCGTAATCCACGGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTTGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCacagtggcg               <  2:462280/149‑9 (MQ=255)
                                                                                                                                    tGGGTTCCGGTAACGACCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGAt             <  1:1126761/149‑1 (MQ=255)
                                                                                                                                    tGGGTTCCGGTAACGACCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGAt             <  1:1126599/149‑1 (MQ=255)
                                                                                                                                     gggTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATa            <  1:991196/149‑1 (MQ=255)
                                                                                                                                               aaCGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTaa  <  2:591650/149‑1 (MQ=255)
                                                                                                                                               aaCGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTaa  <  1:894198/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAA  >  CP009273/796198‑796489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: