Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,911,651 C→T 8.3% intergenic (‑26/+25) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,911,6510CT 8.3% 223.8 / 9.6 89intergenic (‑26/+25)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (43/37);  new base T (3/4);  total (47/42)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

CATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACG  >  CP009273/3911509‑3911796
                                                                                                                                               |                                                                                                                                                 
cATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCt                                                                                                                                             <  2:523807/149‑1 (MQ=255)
  tGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGCCCCGTTCCATTGCGTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTaa                                                                                                                                           <  1:648345/149‑1 (MQ=255)
  tGCGATCCTGAGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GCATCTTGCGATCTTAATACGGATCTCTTTTGCGCAGGCCATGAGCTATTCCTCAATGCATTCAGTCCTGGCCTaa                                                                                                                                           >  2:910840/1‑149 (MQ=255)
   gCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTTCGGCCTGCCCTAAg                                                                                                                                          >  1:317670/1‑149 (MQ=255)
   gCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTCAg                                                                                                                                          >  2:666359/1‑149 (MQ=255)
    cGATCCGGCGATTTCCGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGTCTTGCGGCTTGccttaggg                                                                                                                                         <  1:580327/149‑7 (MQ=255)
     gATCCTGCGATTTACGCATTTTGAAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTATTACGTATCTCTTATGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGc                                                                                                                                        >  2:849857/1‑149 (MQ=255)
          tGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGAGCTTTTGAGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgcc                                                                                                                                   >  2:806432/1‑149 (MQ=255)
           gCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccg                                                                                                                                  <  1:857556/149‑1 (MQ=255)
              aTTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgcaa                                                                                                                               <  1:1117476/149‑3 (MQ=255)
              aTTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCa                                                                                                                               >  1:1235731/1‑149 (MQ=255)
               tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                                                              <  2:131668/149‑1 (MQ=255)
               tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGAAAACCGCCAg                                                                                                                              >  1:1170274/1‑149 (MQ=255)
                     cATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTCCGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCGGCCCTAAGGCAGGCCGCCAgcgctta                                                                                                                        >  1:670448/1‑143 (MQ=255)
                        tttGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCGTAAGGCAAGCCGCCAGACGTTACCa                                                                                                                     <  1:622186/149‑1 (MQ=255)
                         ttGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAg                                                                                                                    >  1:271477/1‑149 (MQ=255)
                               gcggcgACCATCTCCATCGCTTTAGTGGTCTTTTGCGTGTTCAG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTgg                                                                                                              <  2:1163036/149‑1 (MQ=255)
                               gcggcgACCATCTCCAGCGCCTTAGTGACCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTgg                                                                                                              <  1:267438/149‑1 (MQ=255)
                                cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTATC‑GGCGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCCGACGTTACCAGGATTggg                                                                                                             >  1:177335/1‑149 (MQ=255)
                                  gcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGCTCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                           >  1:169699/1‑149 (MQ=255)
                                  gcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                           >  1:974439/1‑149 (MQ=255)
                                  gcgACCATCTCCATCGCTTTAGTGAGCTTTAGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                           >  1:640783/1‑149 (MQ=255)
                                   cgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTg                                                                                                          <  1:1114199/149‑1 (MQ=255)
                                        atgtgcATCGCTTTAGTGTTCTTTTGCGTGTTCTG‑TACGCTTGCGATCTTACTACGTATCTCTTTGGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGGGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTg                                                                                                     <  2:986480/144‑1 (MQ=255)
                                         tctcCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGa                                                                                                    <  2:825623/149‑1 (MQ=255)
                                          tcccATCTCTGTAGTTTTCTTTTGCCGGTTCTT‑GACGCTTGCGATCTAACTACGTATCTCTTTTGCGCCGTCCATGCGCTTCTCCTCGATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGaa                                                                                                   <  2:63394/147‑1 (MQ=255)
                                          ctctATCGCCTTAGCGATGTGTTGGCTGGTCTT‑GACGCTTGCGATCTTACTACGTTTCTTCTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCGTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGaa                                                                                                   <  2:549082/149‑1 (MQ=255)
                                           tcCATCGCTTTAGTGCTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGAATTGGGTTGCTTTGAAg                                                                                                  >  2:143806/1‑149 (MQ=255)
                                           tcCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                  >  1:211235/1‑149 (MQ=255)
                                           tcCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAACCCGCCAGACGTTACCAGGCTTGGGTTGCTTTGAAg                                                                                                  >  2:498956/1‑149 (MQ=255)
                                            ccATCGCTTTAGTGATCTTTTGCGAGTTATG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATTAGATTCTGCGCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                                                                 >  1:926594/1‑149 (MQ=255)
                                             cATCGCTTTAGTGATCTTTTGCGTGAAATGAGAAG‑TTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                                                                >  1:1226397/1‑149 (MQ=255)
                                                  cTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAttgg                                                                                           >  1:167340/1‑146 (MQ=255)
                                                  cTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGa                                                                                           <  1:857455/149‑1 (MQ=255)
                                                     tcgTGATCTTTTGCGTGTTATG‑GACGCTTGCGATCTTACTACGTGTCTCTTTTGCGCCGGCCGTGAGCTTCTCCTCAATGCCTTGCGGCGTGCGCTAAGGCAAGGCGCCAGACGTTGCCAGGGTTGGGTTGCTTTGAAGTGATCGAGGa                                                                                        >  2:212281/3‑149 (MQ=255)
                                                     tAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTCAGGCAAGCCGCCACACGTTACCAGGCTTGGGTTGCTTTGAAGGACACGAGGa                                                                                        >  2:469569/1‑149 (MQ=255)
                                                     tAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAggg                                                                                        >  2:1131692/1‑148 (MQ=255)
                                                         taTCTTTTGCCGTTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGcc                                                                                    <  2:57171/148‑1 (MQ=255)
                                                          aTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGTGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTACCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                   >  1:837474/1‑149 (MQ=255)
                                                          aTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                   >  1:74014/1‑149 (MQ=255)
                                                          aTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGGATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                   >  1:728615/1‑149 (MQ=255)
                                                              tttGCGTGTTCTG‑CACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCCCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCa                                                                               <  1:26151/149‑1 (MQ=255)
                                                                  cGTTTTCTG‑GACGCTTGCGATCTTACTACGTATCCCTTGTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTAAAAAAAAACGAAGGAATCGAGGATGCCTTTCAGCtt                                                                           <  2:269117/149‑1 (MQ=255)
                                                                  cGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCtt                                                                           >  1:929730/1‑149 (MQ=255)
                                                                    tgtTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGc                                                                         <  1:767330/149‑1 (MQ=255)
                                                                        cgg‑gACGCTTGCGTTTTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTc                                                                     <  1:1028478/147‑1 (MQ=255)
                                                                            gACGCTTGCGATCTTATTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCTTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                                                                  <  1:8335/149‑1 (MQ=255)
                                                                               tcTTTCGATTTTATTCCGTTTTTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATCCCTTGCGGCCTGCCCTAAGTTAAGCTGTCACACGTTACCATGATTGCGTTGCTTTGAAGGAATCGAGTATTCCTTTCAGCTTGCCTTCGATTtc                                                               <  2:666203/148‑1 (MQ=255)
                                                                               gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtc                                                               <  2:395690/149‑1 (MQ=255)
                                                                                cTTGCGATCTTACTACGTACCTCTTTTGCGCCGGCCATGATCTTCTCCCCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTTGATTtcg                                                              <  2:842314/149‑1 (MQ=255)
                                                                                   tcGACCTTACTACGTATCTCCTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTGCAGCTTGCCTTCGATTtcgtcg                                                           <  2:167396/148‑1 (MQ=255)
                                                                                   gCGATCTTACTACGTATCTCTTTTGCGGCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCGAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCATTTCAGCTTGCCTTCGATTtcgtcg                                                           <  1:619695/149‑1 (MQ=255)
                                                                                   gCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                           <  1:1006427/149‑1 (MQ=255)
                                                                                   gCGATCTTACTACGTATCTCTGTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                           <  1:505105/149‑1 (MQ=255)
                                                                                    cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTcgtcgt                                                          <  1:79844/149‑1 (MQ=255)
                                                                                    cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTcgtcgt                                                          <  1:112521/149‑1 (MQ=255)
                                                                                      aTCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCGCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGGTTGCCTTCGATTTCGTCGTTg                                                        >  2:266813/1‑149 (MQ=255)
                                                                                         ttACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGGCGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGtgg                                                     >  2:133178/1‑147 (MQ=255)
                                                                                         ttACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg                                                     >  1:461820/1‑149 (MQ=255)
                                                                                            ctacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCa                                                  <  2:974439/149‑1 (MQ=255)
                                                                                               cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCg                                               <  1:926815/149‑1 (MQ=255)
                                                                                                     tctTTTGCGCCGGCCATGAGCTTCTCCGCAAGGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                         <  1:205955/149‑1 (MQ=255)
                                                                                                       ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                       >  2:771461/1‑149 (MQ=255)
                                                                                                       ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                       >  1:584570/1‑149 (MQ=255)
                                                                                                       ttttGCGCCGGCCATGAGCTTCACCTCAATGACTTGCGGCCTGCACTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGCAGGAATCGAGGCAGCCGTTCCGCTTGCCTTCGATTTCGGCGTGGTAGCCACCGGGCTGGtg                                       >  2:929729/1‑148 (MQ=255)
                                                                                                        tttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCCGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGGTTGCCTTCGATTTCGTCGTTGTAGCCACCGGGCTGGTTg                                      >  1:373723/1‑149 (MQ=255)
                                                                                                         ttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGa                                     >  1:217633/1‑149 (MQ=255)
                                                                                                          tGCGCCCGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAt                                    <  2:493981/149‑1 (MQ=255)
                                                                                                             gcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAGCGGGCCGGTTGAtctc                                 >  1:188006/1‑149 (MQ=255)
                                                                                                               cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAGGCCGCCAGACGTTACCGGGGTTGGGCTGCTTTGAAGGAATCGAGGAGGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCCCCGGTCTGCTTGATCTCtt                               >  2:949954/1‑149 (MQ=255)
                                                                                                                  tcATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCa                            <  1:178740/148‑1 (MQ=255)
                                                                                                                  ccctGAGCTTCCTCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTCGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCa                            <  1:254071/146‑1 (MQ=255)
                                                                                                                     tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAAGACGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTAATCTCTTGCATCa                         >  1:239258/1‑149 (MQ=255)
                                                                                                                     tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATGGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCCCTTGCATCa                         >  2:28230/1‑149 (MQ=255)
                                                                                                                       aacTTCGCCGCAATGCCTTGCGGCCGGCCGTAAGGGCAGCCGCCAGACGTTACGAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTCTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGGTGATCTCTTGCATCAAc                       <  2:916400/147‑1 (MQ=255)
                                                                                                                            ctcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGc                  <  2:584570/149‑1 (MQ=255)
                                                                                                                               ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTTATCTCTTGCATCAACGGAGCGTg               >  1:1105266/1‑149 (MQ=255)
                                                                                                                                tcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTCGTAACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa              >  1:1152506/1‑149 (MQ=255)
                                                                                                                                  aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCGTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc            <  1:824019/149‑1 (MQ=255)
                                                                                                                                     gCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAACGGTCTGGGTGATCTCTTGCGTCAACGGAGCGTGATCACg         >  1:679464/1‑149 (MQ=255)
                                                                                                                                      ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGGCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGACAACgg        >  2:922953/1‑149 (MQ=255)
                                                                                                                                      ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAACGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGCTCTCTTGCATCAACgggcagtgagaccgg        >  1:834615/1‑136 (MQ=255)
                                                                                                                                       cTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt       <  2:1226397/149‑1 (MQ=255)
                                                                                                                                       cTTGCGGCCTGCCCTAAGGCAAGCCGCCACACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt       <  2:271477/149‑1 (MQ=255)
                                                                                                                                         ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCg     >  1:1142785/2‑149 (MQ=255)
                                                                                                                                          gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGAGCACGGTCGa    >  1:98212/1‑149 (MQ=255)
                                                                                                                                           cGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGCTCACGGTCGAc   >  2:629972/1‑149 (MQ=255)
                                                                                                                                           cGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGAc   <  2:24142/149‑1 (MQ=255)
                                                                                                                                            ggccggccCTAAGGCAAGCCGCCAGACGTTACCAGGATGGGGTAGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTGGCCACCGGTCTGGTTGATCTCTTGCTTCAACGGAGCGTGATCACGGTCGACg  <  2:641892/149‑1 (MQ=255)
                                                                                                                                            ggCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACg  >  2:584223/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CATGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACG  >  CP009273/3911509‑3911796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑