New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 64196330 (0.630)21 (0.480)
+AGAA		 5/94 NT 36.3% coding (653/1239 nt) ybdR predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
?W3110S.gb = 4186745 50 (1.050)coding (255/420 nt) pioO part of gsp divergon involved in type II protein secretion

CTCGGTGCGGAACAGA‑TTTTTGTTGTTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/641935‑641963
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGTTTTTTGAAG  <  W3110S.gb/4186745‑4186733
                              ||||             
aaCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  >  1:2349577/3‑47
  CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGA    <  1:1354047/43‑1
  CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:1861547/42‑1
  CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:2069484/42‑1
   GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:3912935/1‑43
   GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:2135925/1‑43
   GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:2153421/41‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  >  1:4389952/2‑38
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:2287302/2‑37
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGA    <  1:3219226/35‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:983475/34‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     >  1:4028366/2‑35
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:2929434/34‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:287086/34‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:167079/34‑1
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  >  1:434571/1‑36
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  <  1:1141968/36‑1
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:364086/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:1424860/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:1328700/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:44519/1‑35
                              ||||             
CTCGGTGCGGAACAGA‑TTTTTGTTGTTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/641935‑641963
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGTTTTTTGAAG  <  W3110S.gb/4186745‑4186733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.