New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 156589653 (1.120)15 (0.430)
+TGTGTAGAAACTG		 3/76 NT 24.3% coding (1552/2400 nt) dos cAMP phosphodiesterase, heme‑regulated
?W3110S.gb 4130555 = 73 (1.540)coding (1114/1164 nt) yhfX predicted amino acid racemase
Rejected: Coverage evenness skew score above cutoff.

TTCTTGCGAATATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1565883‑1565896
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGAAATCGTCGGT  >  W3110S.gb/4130555‑4130569
              |||||||||||||               
aTCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGT      <  1:2735956/37‑1
aTCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGT      <  1:569581/37‑1
aTCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGT      <  1:705792/37‑1
aTCTTGCGAATATATGTGTAGAAACTGCCGGAAAT         >  1:1336541/2‑35
aTCTTGCGAATATATGTGTAGAAACTGCCGGAAAT         >  1:1421085/2‑35
aTCTTGCGAATATATGTGTAGAAACTGCCGGAAAT         >  1:3962297/2‑35
 TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGtg  <  1:3709584/41‑3
 TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    <  1:1773850/39‑1
 TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    <  1:2413392/39‑1
 TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    <  1:2776170/39‑1
 TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    >  1:318617/1‑39
   TTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    <  1:2900783/37‑1
   TTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    <  1:3542270/37‑1
     GCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    >  1:3511472/1‑35
     GCGAATATATGTGTAGAAACTGCCGGAAATCGTCG    >  1:717876/1‑35
              |||||||||||||               
TTCTTGCGAATATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1565883‑1565896
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGAAATCGTCGGT  >  W3110S.gb/4130555‑4130569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.