Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178180 2178181 2–1199 69 [0] [0] 63 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)48 (1.120) 29/92 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

GTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176909‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250
                                                                                                                                               
GTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                          <  1:3617853/71‑1
    TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:705141/70‑1
    TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       >  1:2228661/1‑70
     TATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGC                                                                      <  1:2275431/70‑1
        CATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                   >  1:2393099/1‑70
         ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGTCTGACGACTTAGCATGA                                                                  <  1:264010/70‑1
          TCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:4350293/1‑71
            ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATC                                                               <  1:4080379/70‑1
             TCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                        >  1:62801/1‑60
             TCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                        >  1:2015819/1‑60
              CCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCC                                                            >  1:3953275/1‑71
                GCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           >  1:114465/1‑70
                  TTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCA                                                         >  1:1815392/1‑70
                  TTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                     >  1:1661701/1‑58
                  TTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                     >  1:1312791/1‑58
                    GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGTCTGACGACTTAGCATGAATCGCCTCACA                                                       <  1:3686960/70‑1
                     CTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAA                                                                 <  1:2451506/59‑1
                      TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:4328956/52‑1
                      TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:437680/52‑1
                      TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       <  1:401515/52‑1
                       GAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:3339528/66‑1
                        AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   <  1:4106196/70‑1
                        AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    <  1:2298000/69‑1
                         AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:2788676/71‑1
                          AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:4198812/70‑1
                               ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:2028337/71‑1
                                CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:1477877/71‑1
                                CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:2308974/71‑1
                                CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:1663076/71‑1
                                 TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          >  1:401273/1‑70
                                   TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCC                                                            >  1:2553210/1‑50
                                   TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                     >  1:309999/1‑41
                                     TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGC                                     <  1:3732355/71‑1
                                     TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                      <  1:2121061/70‑1
                                       TGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       >  1:3694530/1‑35
                                         TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                  <  1:533841/70‑1
                                               TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATTCGGTA                                               <  1:2626752/51‑1
                                               TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:4392795/42‑1
                                               TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:1685230/42‑1
                                               TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:2646004/42‑1
                                                TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                       <  1:1709945/58‑1
                                                 AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:733490/1‑71
                                                 AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:2545394/1‑55
                                                 AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:2323716/1‑55
                                                 AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               >  1:536110/1‑49
                                                 AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   >  1:1154417/1‑45
                                                 AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        >  1:200505/1‑40
                                                  ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGC                        <  1:2244497/71‑1
                                                  ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         <  1:2871894/70‑1
                                                    AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:475112/71‑1
                                                     GATAAATGGCTTACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                    <  1:4370377/56‑1
                                                     GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                       <  1:2588399/53‑1
                                                      ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                    >  1:2086183/1‑71
                                                       TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                     <  1:564034/69‑1
                                                       TAAATGGCTGACGACTTAGCATGAATCGCCTCACA                                                       <  1:4380105/35‑1
                                                       TAAATGGCTGACGACTTAGCATGAATCGCCTCACA                                                       <  1:1880181/35‑1
                                                        AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                  >  1:2286641/1‑71
                                                        AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                  >  1:2251261/1‑71
                                                        AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                  >  1:2200067/1‑71
                                                        AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 >  1:4391527/1‑56
                                                             GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              >  1:251471/1‑70
                                                               TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:2912245/40‑1
                                                               TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:1037720/40‑1
                                                                 ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG          <  1:226897/70‑1
                                                                   GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA       <  1:1614263/71‑1
                                                                   GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA       >  1:3145450/1‑71
                                                                   GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         <  1:528792/53‑1
                                                                     CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATT     >  1:3903687/1‑71
                                                                      TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG          <  1:677671/65‑1
                                                                      TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                     <  1:4338626/54‑1
                                                                        AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:2165125/1‑71
                                                                        AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT   >  1:2476759/1‑70
                                                                                                                                               
GTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176909‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.