Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2320795–2322125 2322130 6–1336 72 [0] [0] 70 insD–[rcsC] insD,insC,[rcsC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 23207940 (0.000)57 (1.360) 31/90 NT 100% intergenic (‑59/+11) rcsC/insD hybrid sensory kinase in two‑component regulatory system with RcsB and YojN/IS2 element protein
?W3110S.gb 2322131 = 0 (0.000)coding (2368/2424 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

TCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2320719‑2320794
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatcgtCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACT  >  W3110S.gb/2322131‑2322197
                                                                                                                                               
TCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGG                                                                          <  1:672490/71‑1
TCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGG                                                                          <  1:884158/71‑1
  CACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAT                                                                        >  1:4177577/1‑71
  CACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGA                                                                         >  1:1481103/1‑70
   ACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATC                                                                       <  1:2246998/71‑1
    CGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCG                                                                      <  1:876600/71‑1
    CGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCG                                                                      <  1:1222706/71‑1
     GACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT                                                                     >  1:676360/1‑71
     GACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT                                                                     >  1:3423209/1‑71
       CCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCG                                                                   <  1:1305463/71‑1
             TCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCC                                                             <  1:2059750/71‑1
               ATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCAT                                                           <  1:1156222/71‑1
               ATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCA                                                            <  1:1867555/70‑1
                TCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATC                                                          <  1:2045420/71‑1
                  ATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTC                                                        <  1:2181465/71‑1
                     TCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     >  1:607770/1‑71
                     TCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     >  1:2984432/1‑71
                     TCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGA                                                      <  1:2986438/70‑1
                        TCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTC                                                              <  1:2466842/59‑1
                        TCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTC                                                              <  1:95724/59‑1
                        TCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCG                                                                   <  1:1162067/54‑1
                         CATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAA                                                  >  1:834199/1‑70
                         CATTATCGCTGACCGCTTTGTCCGTCGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     <  1:4341848/67‑1
                         CATTATCGCTGACCGCTTTGTCCGTCGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     <  1:992854/67‑1
                           TTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATA                                               <  1:1215446/71‑1
                           TTATCGCTGACCGCTTTGTCCGTTGACGGAAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATA                                               <  1:2461752/71‑1
                             ATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAA                                              <  1:911658/70‑1
                             ATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCAAT                                                           <  1:4356918/57‑1
                              TCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             <  1:2827387/70‑1
                              TCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             <  1:1212280/70‑1
                              TCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             <  1:1615529/70‑1
                              TCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             <  1:3325637/70‑1
                                    ACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGC                                      <  1:494878/71‑1
                                       GCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAA                                   <  1:3945187/71‑1
                                       GCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAA                                   <  1:3940173/71‑1
                                       GCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             <  1:2387477/61‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCTCCAAC                                  <  1:3083819/71‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:4234643/71‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:4378650/71‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:514805/71‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:1039192/71‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAA                                   >  1:522155/1‑70
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAA                                   >  1:1453324/1‑70
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             <  1:45647/60‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAA                                                  <  1:216385/55‑1
                                        CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAA                                                  <  1:1194133/55‑1
                                         TTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  >  1:3538216/1‑70
                                          TTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                             >  1:531203/1‑58
                                           TGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAA                                >  1:3499739/1‑70
                                           TGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACA                                 <  1:2260521/69‑1
                                           TGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAAT                                                >  1:4033286/1‑54
                                            GTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATG                              >  1:952475/1‑71
                                            GTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAAT                               <  1:2054277/70‑1
                                            GTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAAT                               <  1:2371858/70‑1
                                            GTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATA                                           >  1:4414247/1‑58
                                               CGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:896220/64‑1
                                                 TTGACGGCAGAGCGTTAGAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:2607657/61‑1
                                                  TGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     >  1:1721752/1‑42
                                                   GACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     >  1:4390312/1‑41
                                                   GACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                     >  1:3228170/1‑41
                                                    ACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGC                      <  1:3907271/71‑1
                                                    ACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGC                      <  1:3577385/71‑1
                                                     CGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCT                     <  1:4225109/71‑1
                                                     CGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAA                                                  <  1:3010721/42‑1
                                                           AGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCG               <  1:887762/71‑1
                                                             CGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGA             <  1:3223931/71‑1
                                                              GTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  >  1:4306874/1‑49
                                                              GTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  >  1:2856053/1‑49
                                                                  GCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGC        <  1:3334141/71‑1
                                                                   CAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGC        >  1:1033715/1‑70
                                                                    AGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCA      <  1:3634312/71‑1
                                                                    AGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAA                                   >  1:471465/1‑42
                                                                      GATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACA    >  1:4132746/1‑71
                                                                      GATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAG            <  1:2791189/63‑1
                                                                      GATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                  <  1:3829547/41‑1
                                                                       ATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAG            <  1:3159993/62‑1
                                                                        gCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACT  <  1:190945/70‑1
                                                                        TCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACT  <  1:3924437/71‑1
                                                                           TCGCTCTCCATCTCGATCAAATAAATACGCGCCAAa                                  <  1:4419491/36‑2
                                                                                                                                               
TCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2320719‑2320794
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatcgtCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACT  >  W3110S.gb/2322131‑2322197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.