Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262332 3262332 1–1199 68 [0] [0] 65 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)34 (0.780) 28/94 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401
                                                                                                                                               
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                          >  1:1747945/1‑71
  TATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                         >  1:3767659/1‑70
       ACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   >  1:2349444/1‑71
       ACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   >  1:3714522/1‑71
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTG                                                                  >  1:2190698/1‑71
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   <  1:1671100/70‑1
           TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:3482687/1‑70
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                <  1:1126143/69‑1
               GAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCTCGTTAACTT                                                                    <  1:2048743/62‑1
               GAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                         >  1:1459140/1‑57
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCTCGTTAA                                                                       >  1:1103974/1‑58
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:1078636/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:1457748/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:1270931/1‑58
                 AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         <  1:3828554/71‑1
                 AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         <  1:2068758/71‑1
                 AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGA                                                         <  1:3370582/71‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        <  1:1251479/71‑1
                    ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:2054219/1‑65
                     TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGG                                                     <  1:3542692/71‑1
                       ATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:2987859/1‑62
                            TCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:1102330/47‑1
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCA                                               <  1:3772157/68‑1
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        >  1:3033905/1‑59
                                CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                           >  1:1912478/1‑70
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAA                                         >  1:3137142/1‑69
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGC                                                                 >  1:2955877/1‑45
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGC                                                                 >  1:2950889/1‑45
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:1586447/40‑1
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      <  1:3006565/40‑1
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:863515/1‑39
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:2167447/1‑71
                                       TCAACTAAATTATGCAAATTCTGCTGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTT                                   >  1:4237880/1‑71
                                        CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCATAAATGAATGGCATCCAAAGCAAT                                        >  1:3948610/1‑65
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:2732605/1‑44
                                           CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:185516/71‑1
                                              AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTC                            <  1:1770871/71‑1
                                              AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCT                              <  1:1086758/69‑1
                                                TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:571644/1‑71
                                                   TGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGTCATCCAAAGCAATCATTTTTTCTTCGTTA                        <  1:1688857/70‑1
                                                   TGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                    <  1:3539161/58‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                   <  1:3948448/71‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGT                      <  1:3618876/68‑1
                                                        ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGA                  <  1:1837886/71‑1
                                                        ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAA                                         >  1:1777767/1‑48
                                                              CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAC             <  1:1724121/70‑1
                                                              CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:1426797/57‑1
                                                              CGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:2898269/57‑1
                                                                  CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                   <  1:2971989/60‑1
                                                                    CGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAA      <  1:2018506/71‑1
                                                                    CGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTT                                <  1:1731967/45‑1
                                                                     GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAA     >  1:4018848/1‑71
                                                                     GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAA     <  1:2589123/71‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    <  1:3208575/71‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:683133/1‑71
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    <  1:4068451/71‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    <  1:2454395/71‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:2205802/1‑71
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAG                       <  1:2271517/52‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAG                       <  1:4091098/52‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     <  1:3380015/38‑1
                                                                       TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:1831752/1‑70
                                                                       TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:3042439/1‑70
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:1691875/1‑71
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATG   <  1:1961982/70‑1
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                        >  1:1645168/1‑49
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               >  1:3382756/1‑42
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:3927065/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:2487508/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:3403519/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTT                                 <  1:2877255/39‑1
                                                                                                                                               
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.