New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 3539665 =92 (1.940)3 (0.070) 3/90 NT 4.0% coding (433/1470 nt) rhaB rhamnulokinase
?W3110S.gb 3539699 = 63 (1.510)coding (467/1470 nt) rhaB rhamnulokinase
Rejected: Frequency below/above cutoff threshold.

CGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3539740‑3539665
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgctcACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATGAACTGGGAATATACC  >  W3110S.gb/3539699‑3539763
                                                                                                                                             
CGGTCAGGCGATAACTGAAGTAATCCTGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGT                                                                        <  1:402392/71‑1
   TCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGC                                                                     <  1:2116866/71‑1
     AGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCT                                                                    <  1:1258817/70‑1
              CTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCT                                                                    <  1:1428355/61‑1
              CTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCT                                                                    <  1:1903474/61‑1
                                AGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGT                                                                        <  1:3482810/39‑1
                                  CAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTG                                                                      >  1:1220046/1‑39
                                  CAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTG                                                                      >  1:2782585/1‑39
                                  CAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTG                                                                      >  1:1818069/1‑39
                                           AGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCACGCTC                                                             >  1:2380425/1‑39
                                                TGTGTGGAATAAGTTCAGGTTGTTGCTCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTG                             <  1:1008664/66‑1
                                                      GAATAAGTTCAGGTTGTTGCTCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGAT                  <  1:4050383/71‑1
                                                                      TTGCTCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATGAACTGGGAATATACC  <  1:3560200/71‑1
                                                                      TTGCTCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATGAACTGGGAATATAC   >  1:3740344/1‑70
                                                                        GCTCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGA                            >  1:1807030/1‑43
                                                                          TCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATGAACTGGGAATATACC  <  1:3349335/67‑1
                                                                          TCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATG                 <  1:3936466/52‑1
                                                                          TCACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATG                 <  1:640155/52‑1
                                                                                                                                             
CGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3539740‑3539665
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgctcACGCTCTGCTGATGCCGGATTACTTCAGTTATCGCCTGACCGGCAAGATGAACTGGGAATATACC  >  W3110S.gb/3539699‑3539763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.