Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 4310365–4311562 4311563 2–1199 67 [0] [0] 68 insH–[alsK] insH,[alsK]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 43103640 (0.000)49 (1.120) 32/94 NT 100% intergenic (‑286/‑69) yjcS/insH predicted alkyl sulfatase/IS5 element protein
?W3110S.gb 4311564 = 0 (0.000)coding (916/1029 nt) alsK D‑allose kinase

ATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310291‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGA  >  W3110S.gb/4311564‑4311633
                                                                                                                                                 
ATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTT                                                                            >  1:1841892/1‑71
   TATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG                                                                         <  1:474405/71‑1
    ATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG                                                                         >  1:3425412/1‑70
       TTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                       >  1:3146443/1‑69
             CTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                               <  1:3032690/71‑1
             CTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                               <  1:2580733/71‑1
               GTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                                  >  1:279955/1‑66
               GTCGTTGGCACAGCGGGACATGGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                                  >  1:4222029/1‑66
                TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGG                                                            >  1:1639960/1‑71
                TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGT                                                              >  1:3221595/1‑69
                TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGT                                                              >  1:1554984/1‑69
                TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGT                                                              >  1:4348934/1‑69
                  GTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGC                                                           >  1:3553163/1‑70
                  GTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGC                                                           >  1:2563962/1‑70
                     GGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAA                                                       <  1:1162135/70‑1
                      GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                      <  1:861823/70‑1
                      GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACT                                                                <  1:3820849/61‑1
                         CAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACG                                                  >  1:4075566/1‑71
                         CAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACA                                                                     >  1:3338384/1‑53
                          AGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGT                                                 <  1:2541600/71‑1
                           GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:1711667/71‑1
                           GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:1958421/71‑1
                               GACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGA                                                                   <  1:880685/49‑1
                                 CATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAA                                                    >  1:304813/1‑61
                                  ATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGC                                         >  1:2591430/1‑71
                                   TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAA                                                    >  1:1496952/1‑59
                                   TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                                  <  1:4282628/46‑1
                                   TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                    <  1:1378956/44‑1
                                   TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTT                                                                           <  1:106933/37‑1
                                    TGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCC                                        >  1:995217/1‑70
                                     GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                              >  1:1429517/1‑63
                                      CGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      >  1:2735392/1‑70
                                      CGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      >  1:582267/1‑70
                                      CGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      <  1:220270/70‑1
                                           CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATAT                                   <  1:3734537/68‑1
                                           CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:3338685/55‑1
                                            TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTG                                 <  1:1609487/69‑1
                                             GTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAA                                                     >  1:1371938/1‑48
                                              TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTG                              <  1:1124974/70‑1
                                              TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTG                              >  1:1485306/1‑70
                                              TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      >  1:2191350/1‑62
                                              TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAAC                                                                 <  1:3591879/36‑1
                                              TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAAC                                                                 <  1:4238260/36‑1
                                               GCCAACGTTTTACTCATGGGGCTTTAGCACAGAAC                                                                 <  1:4243157/35‑1
                                                CCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCA                            <  1:1759219/70‑1
                                                  AACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCC                         <  1:2358884/71‑1
                                                  AACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCC                         <  1:4123566/71‑1
                                                    CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTG                       <  1:604056/71‑1
                                                    CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                        >  1:1837516/1‑70
                                                     GTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                              >  1:3876180/1‑47
                                                     GTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                              >  1:659469/1‑47
                                                     GTTTTACTCATGGGGCTTTAGCACAGAACTGAGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                        >  1:2699167/1‑69
                                                      TTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:4305421/44‑1
                                                        TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCG                   >  1:4221921/1‑71
                                                                GGGGCTTTAGCACAGAACTGTGGCAAAAAAACGTTGA                                              >  1:649106/1‑37
                                                                GGGGCTTTAGCACAGAACTGTGGCAAAAAAACGTTGA                                              >  1:3890061/1‑37
                                                                 GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAG          <  1:3680984/71‑1
                                                                 GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAG          <  1:152248/71‑1
                                                                 GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAG          <  1:3676025/71‑1
                                                                       TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGA     <  1:3282049/70‑1
                                                                       TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG      <  1:3451438/69‑1
                                                                       TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAA            >  1:527870/1‑63
                                                                        AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA    <  1:4402698/70‑1
                                                                        AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA    <  1:3023271/70‑1
                                                                        AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG      >  1:3051271/1‑68
                                                                         GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGA  <  1:4085840/71‑1
                                                                         GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG   <  1:607160/70‑1
                                                                         GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG   <  1:2470433/70‑1
                                                                         GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG   <  1:3826635/70‑1
                                                                                                                                                 
ATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310291‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGA  >  W3110S.gb/4311564‑4311633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.