Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3680GA100.0% 180.9 / NA 64pseudogene (501/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/35);  total (29/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGT  >  W3110S.gb/3824303‑3824433
                                                                 |                                                                 
gAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACg                                                               <  1:3073902/70‑1 (MQ=255)
  aaaCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                            <  1:3928350/71‑3 (MQ=255)
  aaaCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                            <  1:2024184/71‑3 (MQ=255)
   aaCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                            >  1:274404/1‑68 (MQ=255)
    aCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggtg                                                            >  1:4196372/1‑67 (MQ=255)
      ggCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg                                                         >  1:1330687/1‑70 (MQ=255)
         aaGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaat                                                     <  1:4306097/71‑4 (MQ=255)
              gAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                                 >  1:3694425/1‑70 (MQ=255)
              gAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                                 >  1:232767/1‑70 (MQ=255)
               aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                               <  1:2884486/71‑1 (MQ=255)
               aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                               >  1:2329814/1‑71 (MQ=255)
               aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                               <  1:1813908/71‑1 (MQ=255)
                   ccAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg                                           <  1:202790/71‑1 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                          <  1:1846119/71‑1 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                          <  1:3809293/71‑1 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                          <  1:2392574/71‑1 (MQ=255)
                    cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTg                                           >  1:1456726/1‑70 (MQ=255)
                      acccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg                                                         >  1:3077161/3‑54 (MQ=255)
                       cccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                        >  1:2823998/2‑70 (MQ=255)
                         cGATGACAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGc                                                        >  1:1887/1‑52 (MQ=255)
                         cGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                                 >  1:2842749/1‑59 (MQ=255)
                          gATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACg                                    <  1:4138096/71‑1 (MQ=255)
                           aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                      <  1:2363009/52‑3 (MQ=255)
                           aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                      <  1:3406528/52‑3 (MQ=255)
                             gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                                      >  1:3574559/1‑66 (MQ=255)
                             gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                                      >  1:4186287/1‑66 (MQ=255)
                             gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                  >  1:195445/1‑70 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                       <  1:3848014/48‑2 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:3877002/70‑1 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 >  1:4378864/1‑70 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:1080610/70‑1 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:2759955/70‑1 (MQ=255)
                              aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:2387139/70‑1 (MQ=255)
                               aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                               <  1:3541650/71‑1 (MQ=255)
                                    aTGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActc                          <  1:4418413/71‑1 (MQ=255)
                                      ggTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAct                           >  1:1583316/1‑68 (MQ=255)
                                       gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                       >  1:3423536/1‑71 (MQ=255)
                                       gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                       >  1:1650945/1‑71 (MQ=255)
                                        tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                       >  1:1458063/1‑70 (MQ=255)
                                         aGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                     >  1:2958070/1‑71 (MQ=255)
                                          gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                          <  1:4310905/49‑1 (MQ=25)
                                          gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                     >  1:3188825/1‑70 (MQ=255)
                                          gCCGCAGTTTCTGTCGGTATTGTAAACCGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                     <  1:3261125/70‑1 (MQ=255)
                                            cGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                     >  1:2442978/1‑68 (MQ=255)
                                             gCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                   <  1:3875356/69‑1 (MQ=255)
                                              cAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                   >  1:2427079/1‑68 (MQ=255)
                                               aGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                  >  1:49513/1‑36 (MQ=16)
                                               aGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                                 <  1:487192/37‑1 (MQ=16)
                                               aGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                                 <  1:1146740/37‑1 (MQ=16)
                                               aGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAgaga               >  1:1288725/1‑71 (MQ=255)
                                                 tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                          <  1:1899496/42‑1 (MQ=19)
                                                 tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                                      <  1:695985/46‑1 (MQ=25)
                                                 tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                                      <  1:3957182/46‑1 (MQ=25)
                                                 tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                   <  1:1046803/65‑1 (MQ=255)
                                                 tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                   <  1:660060/65‑1 (MQ=255)
                                                  ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActcag                        >  1:452878/1‑57 (MQ=255)
                                                  ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActcag                        >  1:474601/1‑57 (MQ=255)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                   >  1:225996/1‑47 (MQ=25)
                                                   tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga                 >  1:269608/1‑65 (MQ=255)
                                                    ctgtctgtATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa           <  1:1316784/70‑1 (MQ=255)
                                                    cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa           <  1:3379863/70‑1 (MQ=255)
                                                         ggtaTTGTAAACGGTAAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGa      <  1:456305/67‑1 (MQ=255)
                                                           taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg   <  1:3738145/70‑1 (MQ=255)
                                                             ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt  <  1:4272502/70‑1 (MQ=255)
                                                                 |                                                                 
GAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGT  >  W3110S.gb/3824303‑3824433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: