| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,410 | T→A | 100% | pseudogene (543/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,410 | 0 | T | A | 100.0% | 206.9 / NA | 72 | pseudogene (543/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (43/29); total (43/29) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACGG > W3110S.gb/3824342‑3824477 | gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc > 1:3423536/1‑71 (MQ=255)gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc > 1:1650945/1‑71 (MQ=255) tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc > 1:1458063/1‑70 (MQ=255) aGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg > 1:2958070/1‑71 (MQ=255) gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg > 1:3188825/1‑70 (MQ=255) gCCGCAGTTTCTGTCGGTATTGTAAACCGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg < 1:3261125/70‑1 (MQ=255) cGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg > 1:2442978/1‑68 (MQ=255) gCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:3875356/69‑1 (MQ=255) cAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa > 1:2427079/1‑68 (MQ=255) aGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAgaga > 1:1288725/1‑71 (MQ=255) tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:1046803/65‑1 (MQ=255) tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:660060/65‑1 (MQ=255) tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga > 1:269608/1‑65 (MQ=255) ctgtctgtATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa < 1:1316784/70‑1 (MQ=255) cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa < 1:3379863/70‑1 (MQ=255) ggtaTTGTAAACGGTAAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGa < 1:456305/67‑1 (MQ=255) taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:3738145/70‑1 (MQ=255) ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt < 1:4272502/70‑1 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGATTCAGCCGCAGAGACCGACATGAACGTAGt > 1:319440/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa > 1:3088690/4‑69 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa > 1:3093857/4‑69 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAg > 1:1960744/4‑70 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAg < 1:735082/67‑1 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:2800096/4‑71 (MQ=255) taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:3023341/3‑58 (MQ=255) taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:3018234/3‑58 (MQ=255) aaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:1856261/2‑71 (MQ=255) aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa > 1:4188759/1‑66 (MQ=255) aaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgat > 1:364552/1‑71 (MQ=255) aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgatg < 1:2866903/71‑1 (MQ=255) aCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:7743/1‑69 (MQ=255) cGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga > 1:3677195/1‑48 (MQ=37) cGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCaga > 1:3773098/1‑48 (MQ=37) gtgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGc < 1:2791894/41‑1 (MQ=25) tgAAGCAATTTGCGATCTGGAATACTTTGAAGACTCAGCCg < 1:2120230/40‑1 (MQ=21) atttGCGATCTGGAATACGTTGAAGACTCATCCGCa < 1:2218332/35‑1 (MQ=37) atttGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:1695353/35‑1 (MQ=38) ttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGa > 1:1206707/1‑47 (MQ=255) ttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgc > 1:2940418/1‑71 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCATCCGCAGAGACCGa > 1:3835102/1‑41 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCaga > 1:1215656/1‑35 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:1055575/1‑41 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:1604701/1‑41 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGAcc > 1:1308395/1‑60 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGAcc > 1:4156377/1‑60 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgcg > 1:993577/1‑71 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgcg > 1:81460/1‑71 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgc < 1:2618532/70‑1 (MQ=255) aTCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGa > 1:1804375/1‑61 (MQ=255) cTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatcat > 1:2587670/1‑71 (MQ=255) cTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatcat > 1:2273313/1‑71 (MQ=255) tGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgatg < 1:2911980/50‑1 (MQ=255) tGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt < 1:3758498/46‑1 (MQ=255) tGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCAtt > 1:4281149/1‑71 (MQ=255) tGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCAtt > 1:2608204/1‑71 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatca < 1:3108948/67‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg < 1:3994253/70‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg > 1:1096093/1‑70 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg < 1:859356/70‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg < 1:3421862/70‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGAGCATCATTg < 1:570732/70‑1 (MQ=255) aCGTTGAAGACTCAGCCGCAGAGACCGACATGAACg < 1:1443354/36‑1 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatc > 1:2516912/1‑61 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTg > 1:2750721/1‑70 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGc < 1:3391473/71‑1 (MQ=255) cGTAGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGa > 1:2424677/1‑51 (MQ=255) gTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGc < 1:123634/70‑1 (MQ=255) ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGa < 1:4287188/49‑1 (MQ=255) ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAg < 1:1559376/71‑1 (MQ=255) tGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:2709826/1‑38 (MQ=255) gAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga > 1:2562774/1‑37 (MQ=255) aCTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGGATCATTGAAGTGCAGGGGACg < 1:2355888/71‑1 (MQ=255) ctcaGCCGCAGAGACCGTCATGAACGTAGTGATGACCg > 1:3235794/1‑38 (MQ=38) ctcaGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAg > 1:658493/1‑64 (MQ=255) ctcaGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAg > 1:1903645/1‑64 (MQ=255) ctcaGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACgg < 1:3007404/71‑1 (MQ=255) | GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACGG > W3110S.gb/3824342‑3824477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |