Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,936,024 C→T 6.7% A123A (GCC→GCT)  dppC → dipeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,936,0240CT 6.7% 227.0 / 4.7 74A123A (GCC→GCT) dppCdipeptide transporter
Reads supporting (aligned to +/- strand):  ref base C (34/35);  new base T (2/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TCGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAG  >  W3110S.gb/3935956‑3936092
                                                                    |                                                                    
tCGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGcc                                                                      <  1:1874050/69‑1 (MQ=255)
tCGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCgg                                                                    >  1:1913728/1‑71 (MQ=255)
tCGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCgg                                                                    >  1:1568620/1‑71 (MQ=255)
tCGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCg                                                                     <  1:2649326/70‑1 (MQ=255)
tCGCTGCTGGTTGGCTGTCTGCTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCgg                                                                    >  1:1291000/1‑71 (MQ=255)
 cGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGTCCTGATCGCCGGt                                                                   >  1:1155913/1‑71 (MQ=255)
  gctgctGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGt                                                                   <  1:842963/70‑1 (MQ=255)
  gctgctGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGt                                                                   >  1:4262672/1‑70 (MQ=255)
  gctgctGGGTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGt                                                                   <  1:1059271/70‑1 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCTGCCTGATCGCCGGTTACtt                                                              >  1:1590533/1‑71 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACtt                                                              >  1:30137/1‑71 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACtt                                                              <  1:2448405/71‑1 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACtt                                                              <  1:4107796/71‑1 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACt                                                               <  1:542574/70‑1 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACt                                                               >  1:3313519/1‑70 (MQ=255)
      ctgGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACt                                                               <  1:3554460/70‑1 (MQ=255)
         gTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACttt                                                             <  1:1654129/69‑1 (MQ=255)
         gTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACttt                                                             <  1:921088/69‑1 (MQ=255)
            ggCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTggcg                                                         <  1:397336/70‑1 (MQ=255)
              cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                      >  1:3714763/1‑71 (MQ=255)
              cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                      >  1:4119274/1‑71 (MQ=255)
              cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                      <  1:219355/71‑1 (MQ=255)
              cTGTCTGGCAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                      <  1:1343121/71‑1 (MQ=255)
                   tGGTAGTGGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTGGTc                                                 >  1:3046853/1‑71 (MQ=255)
                   tGGTAGTGGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTATGGTc                                                 >  1:347243/1‑71 (MQ=255)
                     gTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTc                                                 <  1:4015792/69‑1 (MQ=255)
                      tAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGAt                                              <  1:975527/71‑1 (MQ=255)
                      tAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGAt                                              <  1:1155208/71‑1 (MQ=255)
                         ttGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGcc                                                                      <  1:4284083/44‑1 (MQ=255)
                           gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCTGCCTGGTCGATAAcat                                         >  1:1004994/1‑71 (MQ=255)
                           gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcat                                         >  1:1140026/1‑71 (MQ=255)
                           gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcat                                         >  1:3680895/1‑71 (MQ=255)
                           gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAca                                          >  1:667959/1‑70 (MQ=255)
                              ttATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatcat                                      <  1:3340169/71‑1 (MQ=255)
                              ttATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatca                                       <  1:1748549/70‑1 (MQ=255)
                               tATCGCTGATTATGGGCCTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTg                                                    >  1:1181761/1‑56 (MQ=255)
                                aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatca                                       <  1:2250318/68‑1 (MQ=255)
                                aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                    >  1:3839948/1‑71 (MQ=255)
                                aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                    >  1:2256703/1‑71 (MQ=255)
                                 tCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                    <  1:1760153/70‑1 (MQ=255)
                                 tCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                    <  1:487658/70‑1 (MQ=255)
                                  cGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATg                                  >  1:2734890/1‑71 (MQ=255)
                                   gCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAc                                           >  1:4195162/1‑61 (MQ=255)
                                   gCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                 <  1:3547085/71‑1 (MQ=255)
                                    cTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcg                                >  1:2518460/1‑71 (MQ=255)
                                       aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcgc                               <  1:1564192/69‑1 (MQ=255)
                                       aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcgc                               <  1:3590858/69‑1 (MQ=255)
                                       aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGcgcg                              <  1:3337973/70‑1 (MQ=255)
                                        ttATGGGCGTTATTCTCGGCCTGATCGCTTGTTACTTTGGCGGTCTg                                                    <  1:730774/47‑1 (MQ=255)
                                        ttATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTg                                                    <  1:2722313/47‑1 (MQ=255)
                                        ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTggcg                                                         >  1:4268464/1‑42 (MQ=255)
                                        ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                 >  1:3266049/1‑66 (MQ=255)
                                        ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                 >  1:2552127/1‑66 (MQ=255)
                                          aTGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGt                          >  1:1442336/1‑71 (MQ=255)
                                          aTGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGt                          >  1:3518040/1‑71 (MQ=255)
                                           tGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTc                         <  1:3945620/71‑1 (MQ=255)
                                             ggCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTGGTCGATAACATCATCATgc                                 <  1:368217/61‑1 (MQ=255)
                                              gCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                       <  1:1034600/70‑1 (MQ=255)
                                               cGTTATTCTTGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTc                                                 <  1:2743555/43‑1 (MQ=255)
                                                 ttATTCTCGGCCTGATCGCCGGTTACTTTggcggc                                                       <  1:273873/35‑1 (MQ=255)
                                                   aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTgg                           >  1:1013225/1‑61 (MQ=255)
                                                   aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                 >  1:2882654/1‑71 (MQ=255)
                                                   aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                 >  1:4224803/1‑71 (MQ=255)
                                                   aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                 >  1:3636888/1‑71 (MQ=255)
                                                     tctcGGCCTGATCGCCGCTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc               >  1:1541398/1‑71 (MQ=255)
                                                      ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc               <  1:3709709/70‑1 (MQ=255)
                                                      ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc               <  1:2061343/70‑1 (MQ=255)
                                                      ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc               <  1:3579525/70‑1 (MQ=255)
                                                      ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt              >  1:4014336/1‑71 (MQ=255)
                                                       tcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTg             >  1:3467299/1‑71 (MQ=255)
                                                         ggCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTgg            <  1:457439/70‑1 (MQ=255)
                                                          gCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCTCGTGGTCGATATCATGCTGgcg          <  1:569004/71‑1 (MQ=255)
                                                               aTCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCTATATCATGCTGGCGCTGcc     <  1:114067/71‑1 (MQ=255)
                                                                tCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGcc     >  1:3098068/1‑70 (MQ=255)
                                                                tCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGcc     >  1:1228909/1‑70 (MQ=255)
                                                                  gCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAg  <  1:597767/71‑1 (MQ=255)
                                                                    |                                                                    
TCGCTGCTGGTTGGCTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAG  >  W3110S.gb/3935956‑3936092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑