Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,936,039 C→T 7.6% G128G (GGC→GGT)  dppC → dipeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,936,0390CT 7.6% 199.7 / 4.7 66G128G (GGC→GGT) dppCdipeptide transporter
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (2/3);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGGCGC  >  W3110S.gb/3935970‑3936106
                                                                     |                                                                   
cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    >  1:3714763/1‑71 (MQ=255)
cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    >  1:4119274/1‑71 (MQ=255)
cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    <  1:219355/71‑1 (MQ=255)
cTGTCTGGCAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    <  1:1343121/71‑1 (MQ=255)
     tGGTAGTGGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTGGTc                                                               >  1:3046853/1‑71 (MQ=255)
     tGGTAGTGGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTATGGTc                                                               >  1:347243/1‑71 (MQ=255)
       gTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTc                                                               <  1:4015792/69‑1 (MQ=255)
        tAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGAt                                                            <  1:975527/71‑1 (MQ=255)
        tAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGAt                                                            <  1:1155208/71‑1 (MQ=255)
             gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCTGCCTGGTCGATAAcat                                                       >  1:1004994/1‑71 (MQ=255)
             gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcat                                                       >  1:1140026/1‑71 (MQ=255)
             gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcat                                                       >  1:3680895/1‑71 (MQ=255)
             gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAca                                                        >  1:667959/1‑70 (MQ=255)
                ttATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatcat                                                    <  1:3340169/71‑1 (MQ=255)
                ttATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatca                                                     <  1:1748549/70‑1 (MQ=255)
                 tATCGCTGATTATGGGCCTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTg                                                                  >  1:1181761/1‑56 (MQ=255)
                  aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatca                                                     <  1:2250318/68‑1 (MQ=255)
                  aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  >  1:3839948/1‑71 (MQ=255)
                  aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  >  1:2256703/1‑71 (MQ=255)
                   tCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  <  1:1760153/70‑1 (MQ=255)
                   tCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  <  1:487658/70‑1 (MQ=255)
                    cGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATg                                                >  1:2734890/1‑71 (MQ=255)
                     gCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAc                                                         >  1:4195162/1‑61 (MQ=255)
                     gCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                               <  1:3547085/71‑1 (MQ=255)
                      cTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcg                                              >  1:2518460/1‑71 (MQ=255)
                         aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcgc                                             <  1:1564192/69‑1 (MQ=255)
                         aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcgc                                             <  1:3590858/69‑1 (MQ=255)
                         aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGcgcg                                            <  1:3337973/70‑1 (MQ=255)
                          ttATGGGCGTTATTCTCGGCCTGATCGCTTGTTACTTTGGCGGTCTg                                                                  <  1:730774/47‑1 (MQ=255)
                          ttATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTg                                                                  <  1:2722313/47‑1 (MQ=255)
                          ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                               >  1:3266049/1‑66 (MQ=255)
                          ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                               >  1:2552127/1‑66 (MQ=255)
                            aTGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGt                                        >  1:3518040/1‑71 (MQ=255)
                            aTGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGt                                        >  1:1442336/1‑71 (MQ=255)
                             tGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTc                                       <  1:3945620/71‑1 (MQ=255)
                               ggCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTGGTCGATAACATCATCATgc                                               <  1:368217/61‑1 (MQ=255)
                                gCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                                     <  1:1034600/70‑1 (MQ=255)
                                 cGTTATTCTTGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTc                                                               <  1:2743555/43‑1 (MQ=255)
                                   ttATTCTCGGCCTGATCGCCGGTTACTTTggcggc                                                                     <  1:273873/35‑1 (MQ=255)
                                     aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTgg                                         >  1:1013225/1‑61 (MQ=255)
                                     aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                               >  1:2882654/1‑71 (MQ=255)
                                     aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                               >  1:4224803/1‑71 (MQ=255)
                                     aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                               >  1:3636888/1‑71 (MQ=255)
                                       tctcGGCCTGATCGCCGCTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             >  1:1541398/1‑71 (MQ=255)
                                        ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             <  1:3579525/70‑1 (MQ=255)
                                        ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             <  1:2061343/70‑1 (MQ=255)
                                        ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             <  1:3709709/70‑1 (MQ=255)
                                        ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt                            >  1:4014336/1‑71 (MQ=255)
                                         tcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTg                           >  1:3467299/1‑71 (MQ=255)
                                           ggCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTgg                          <  1:457439/70‑1 (MQ=255)
                                            gCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCTCGTGGTCGATATCATGCTGgcg                        <  1:569004/71‑1 (MQ=255)
                                                 aTCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCTATATCATGCTGGCGCTGcc                   <  1:114067/71‑1 (MQ=255)
                                                  tCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGcc                   >  1:3098068/1‑70 (MQ=255)
                                                  tCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGcc                   >  1:1228909/1‑70 (MQ=255)
                                                    gCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAg                <  1:597767/71‑1 (MQ=255)
                                                       ggTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTct             >  1:1013616/1‑71 (MQ=255)
                                                        gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                                     <  1:3303091/46‑1 (MQ=255)
                                                        gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt                            <  1:2895702/55‑1 (MQ=255)
                                                        gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt                            <  1:443581/55‑1 (MQ=255)
                                                        gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctg            >  1:1178111/1‑71 (MQ=255)
                                                         ttACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCa                                <  1:1112222/50‑1 (MQ=255)
                                                         ttACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgc           <  1:1713914/71‑1 (MQ=255)
                                                         ttACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgc           <  1:2934607/71‑1 (MQ=255)
                                                          tACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgc           >  1:4288627/1‑70 (MQ=255)
                                                           aCTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgctg         >  1:3927419/1‑71 (MQ=255)
                                                           aCTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgct          <  1:2257480/70‑1 (MQ=255)
                                                            cTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgctgc        <  1:1501718/71‑1 (MQ=255)
                                                             tttGGCGGCCTGGTCGATAACATCATTATGCGCGt                                           <  1:2247681/35‑1 (MQ=255)
                                                             tttGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                                     <  1:4181587/41‑1 (MQ=255)
                                                               tGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgctgctg      <  1:1546871/70‑1 (MQ=255)
                                                               tGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTgg     >  1:1258791/1‑71 (MQ=255)
                                                                ggcggcCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGgc    >  1:1871090/1‑71 (MQ=255)
                                                                  cggcCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGgcgc  <  1:4393669/71‑1 (MQ=255)
                                                                     |                                                                   
CTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGGCGC  >  W3110S.gb/3935970‑3936106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑