| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,034,547 | T→C | 8.8% | L40L (TTA→TTG) | yhhN ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,034,547 | 0 | T | C | 8.8% | 175.9 / 8.2 | 68 | L40L (TTA→TTG) | yhhN | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (28/34); new base C (2/4); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GCAGTGAGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCACGATACG > W3110S.gb/4034477‑4034611 | gcagTGAGGCGCACAGCCCTGCCAGCACGAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGt < 1:2552899/71‑1 (MQ=255)gcagTGAGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGt < 1:4426396/71‑1 (MQ=255)gcagTGAGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGt < 1:3360476/71‑1 (MQ=255) gTGAGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTaa > 1:3992887/1‑70 (MQ=255) aGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAAcagc < 1:4319658/71‑1 (MQ=255) aGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAAcag > 1:1801929/1‑70 (MQ=255) aGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAAcag > 1:843451/1‑70 (MQ=255) caGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGgag > 1:486369/1‑69 (MQ=255) cTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAgg < 1:3891402/71‑1 (MQ=255) tGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGga < 1:884532/62‑1 (MQ=255) gCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGt > 1:4213239/1‑71 (MQ=255) gCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTaa > 1:407953/1‑49 (MQ=255) gCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAgg < 1:791933/56‑1 (MQ=255) cACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACg > 1:2017680/1‑71 (MQ=255) aCCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACgg > 1:2385027/1‑71 (MQ=255) ccAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTaa > 1:1978653/1‑46 (MQ=255) ccAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTaa > 1:3532208/1‑46 (MQ=255) ccAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAAcagcag < 1:1867296/52‑1 (MQ=255) gATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTaa > 1:1444622/1‑43 (MQ=255) gATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTa < 1:51950/71‑1 (MQ=255) aTAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGttaa > 1:4058906/1‑68 (MQ=255) tAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTaaa > 1:3145579/1‑71 (MQ=255) aGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTaaa > 1:3379419/1‑70 (MQ=255) aGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTaaa > 1:1612749/1‑70 (MQ=255) gCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGt < 1:1637121/57‑1 (MQ=255) gCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGt < 1:4430408/57‑1 (MQ=255) gCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAac < 1:3874384/70‑1 (MQ=255) cTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAacac > 1:4050222/1‑71 (MQ=255) cTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGCAACAGCAGGAGAAGAAGGGTTAACGGTTTa < 1:2548057/66‑1 (MQ=255) cTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGCAACAGCAGGAGAAGAAGGGTTAACGGTTTa < 1:3275297/66‑1 (MQ=255) cTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGCAACAGCAGGAGAAGAAGGGTTAACGGTTTa < 1:2862067/66‑1 (MQ=255) cTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGCAACAGCAGGAGAAGAAGGGTTAACGGTTTa < 1:2415941/66‑1 (MQ=255) tAATGGCGGCGAACATCGGCGCTTGCCAGGCCAGTAAca < 1:3555396/39‑1 (MQ=255) tGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTa < 1:1955171/62‑1 (MQ=255) gCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTaa > 1:2232726/1‑61 (MQ=255) gCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCg > 1:651816/1‑71 (MQ=255) tCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAgaagaa < 1:1233340/43‑1 (MQ=255) tCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGttt < 1:2528295/56‑1 (MQ=255) gAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGcc < 1:1263017/71‑1 (MQ=255) gAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGc > 1:1795012/1‑70 (MQ=255) gAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGc > 1:3875835/1‑70 (MQ=255) aaCATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAAc < 1:1750234/48‑1 (MQ=255) aaCATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGttt < 1:699063/53‑1 (MQ=255) aaCATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCa < 1:2588567/71‑1 (MQ=255) aaCATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCa < 1:3950726/71‑1 (MQ=255) aCATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAg > 1:933070/1‑71 (MQ=255) aCATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAg > 1:3413027/1‑71 (MQ=255) cATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAgg > 1:774135/1‑71 (MQ=255) cATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAg > 1:779450/1‑70 (MQ=255) ttCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAAc < 1:722311/44‑1 (MQ=255) aTCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGt < 1:3090087/71‑1 (MQ=255) aTCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGt < 1:2927800/71‑1 (MQ=255) ggCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAgg > 1:513627/1‑36 (MQ=255) ggCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAgg > 1:2321896/1‑36 (MQ=255) ggCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTgg < 1:3078759/71‑1 (MQ=255) cgcTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGtt > 1:409761/1‑37 (MQ=255) gcTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGccc < 1:18820/71‑1 (MQ=255) gccaggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCac < 1:3465777/69‑1 (MQ=255) ccaggccagTAACAGCAGGATAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgat < 1:4399369/71‑1 (MQ=255) ccaggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCGAGCGTTGCCAGGTTGGCCCacgat < 1:2175101/71‑1 (MQ=255) caggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgata > 1:3587162/1‑71 (MQ=255) aggccagTAACAGCAGTAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgatac < 1:1295389/71‑1 (MQ=255) aggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgatac < 1:4281455/71‑1 (MQ=255) aggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgatac < 1:1790140/71‑1 (MQ=255) aggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgatac < 1:4037899/71‑1 (MQ=255) aggccagCAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCg > 1:4400182/1‑49 (MQ=255) aggccagCAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCg > 1:4293322/1‑49 (MQ=255) ggccagTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacgatacg < 1:1690370/71‑1 (MQ=255) agTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCacga < 1:3696121/63‑1 (MQ=255) gTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGc < 1:77079/42‑1 (MQ=255) gTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAg < 1:1735316/50‑1 (MQ=255) | GCAGTGAGGCGCACAGCCCTGCCAGCACCAGATAGCTAATGGCGTCGAACATCGGCGCTTGCCAGGCCAGTAACAGCAGGAGAAGAAGGGTTAACGGTTTAAACACCCAGCGTTGCCAGGTTGGCCCACGATACG > W3110S.gb/4034477‑4034611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |