| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,177,230 | C→T | 8.2% | D89N (GAT→AAT) | gspK ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,177,230 | 0 | C | T | 8.2% | 181.3 / 4.8 | 61 | D89N (GAT→AAT) | gspK | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (29/27); new base T (2/3); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
ATTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACG > W3110S.gb/4177174‑4177300 | aTTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCa < 1:3993453/68‑1 (MQ=255) ttGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGc < 1:1230924/63‑1 (MQ=255) gagaTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgtt > 1:560367/1‑71 (MQ=255) gagaTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgt < 1:1891067/70‑1 (MQ=255) gaTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgttgt < 1:3131860/71‑1 (MQ=255) gaTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgttgt < 1:1340085/71‑1 (MQ=255) aTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACt < 1:820513/65‑1 (MQ=255) ttAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTgg < 1:764987/71‑1 (MQ=255) ttAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTgg < 1:513414/71‑1 (MQ=255) tAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGc > 1:274791/1‑71 (MQ=255) aaaaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCt > 1:2521245/1‑71 (MQ=255) aaaaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCt > 1:362480/1‑71 (MQ=255) aaaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCt < 1:817325/70‑1 (MQ=255) aaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTACAGCTGCAACTGTTGTGGCtgt < 1:1778612/71‑1 (MQ=255) aCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTgg < 1:3260007/66‑1 (MQ=255) aGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTTTGGCTGTGcc < 1:588914/71‑1 (MQ=255) gTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGccc < 1:435361/71‑1 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:4001299/1‑70 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:3171283/1‑70 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:944627/1‑70 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:2201822/1‑70 (MQ=255) gggCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTAc > 1:650065/1‑71 (MQ=255) ggCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTAct < 1:221556/71‑1 (MQ=255) ggCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTAct > 1:3199629/1‑71 (MQ=255) tCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGc < 1:2906205/42‑1 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgt > 1:4253867/1‑46 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgt > 1:3576519/1‑46 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgt > 1:1764002/1‑46 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACtgctgc > 1:2561590/1‑70 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACtgctgc > 1:4297783/1‑70 (MQ=255) tAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgt < 1:3297955/43‑1 (MQ=255) gCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtc > 1:627478/1‑71 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt < 1:725319/71‑3 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt > 1:2431399/1‑69 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt > 1:4013523/1‑69 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt < 1:4030693/71‑3 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt < 1:882044/71‑3 (MQ=255) cctCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctgt > 1:4341851/3‑70 (MQ=255) cTTCACTGTATTGCCATCTTCCATCTGCAACTGTTGTTGCTGTTCCCAg < 1:521503/49‑1 (MQ=255) cTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCa < 1:1482204/59‑1 (MQ=255) cTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctgtc > 1:451569/1‑71 (MQ=255) ttCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctg < 1:3548315/68‑1 (MQ=255) tCACTGTATTGCCATCTTCCAGTTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGtctgtctg > 1:1836292/1‑71 (MQ=255) tCACTGTATTGACATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTa > 1:1174001/1‑49 (MQ=255) cACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTa > 1:2609270/1‑48 (MQ=255) cACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTctgtctgt > 1:2846190/1‑71 (MQ=255) cACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTctgtctgt > 1:3987804/1‑71 (MQ=255) aCTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTg > 1:4201991/1‑60 (MQ=255) tATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGtt < 1:4174818/70‑1 (MQ=255) tATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGtt < 1:284345/70‑1 (MQ=255) aTTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTa > 1:3087324/1‑70 (MQ=255) ttGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTAt < 1:3944301/70‑1 (MQ=255) ttGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATc > 1:2471506/1‑71 (MQ=255) gCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGtt < 1:2736415/66‑1 (MQ=255) gCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCt < 1:4108917/70‑1 (MQ=255) cATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTAt > 1:1347052/1‑66 (MQ=255) cATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCtt > 1:3921157/1‑69 (MQ=255) aTCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTAt < 1:3921078/65‑1 (MQ=255) tCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCa < 1:4236001/43‑1 (MQ=255) tCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTg < 1:837752/48‑1 (MQ=255) tCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctgt > 1:775225/1‑54 (MQ=255) cTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACg > 1:2192045/1‑71 (MQ=255) | ATTGAGATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACG > W3110S.gb/4177174‑4177300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |