| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,177,249 | T→C | 10.9% | P82P (CCA→CCG) | gspK ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,177,249 | 0 | T | C | 10.9% | 160.8 / 11.1 | 64 | P82P (CCA→CCG) | gspK | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base T (32/25); new base C (4/3); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
GATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAA > W3110S.gb/4177179‑4177319 | gaTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgttgt < 1:3131860/71‑1 (MQ=255)gaTTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACtgttgt < 1:1340085/71‑1 (MQ=255) ttAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTgg < 1:513414/71‑1 (MQ=255) ttAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTgg < 1:764987/71‑1 (MQ=255) tAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGc > 1:274791/1‑71 (MQ=255) aaaaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCt > 1:2521245/1‑71 (MQ=255) aaaaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCt > 1:362480/1‑71 (MQ=255) aaaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCt < 1:817325/70‑1 (MQ=255) aaCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTACAGCTGCAACTGTTGTGGCtgt < 1:1778612/71‑1 (MQ=255) aCAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTgg < 1:3260007/66‑1 (MQ=255) aGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTTTGGCTGTGcc < 1:588914/71‑1 (MQ=255) gTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGccc < 1:435361/71‑1 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:3171283/1‑70 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:2201822/1‑70 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:4001299/1‑70 (MQ=255) cTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAg > 1:944627/1‑70 (MQ=255) gggCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTAc > 1:650065/1‑71 (MQ=255) ggCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTAct > 1:3199629/1‑71 (MQ=255) ggCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTAct < 1:221556/71‑1 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACtgctgc > 1:2561590/1‑70 (MQ=255) gcgTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACtgctgc > 1:4297783/1‑70 (MQ=255) gCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtc > 1:627478/1‑71 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt < 1:4030693/71‑3 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt > 1:2431399/1‑69 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt > 1:4013523/1‑69 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt < 1:725319/71‑3 (MQ=255) ccACTTCACTGTATTGCCATTTTCCAGCTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGgttt < 1:882044/71‑3 (MQ=255) cctCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctgt > 1:4341851/3‑70 (MQ=255) cTTCACTGTATTGCCATCTTCCATCTGCAACTGTTGTTGCTGTTCCCAg < 1:521503/49‑1 (MQ=255) cTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCa < 1:1482204/59‑1 (MQ=255) cTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctgtc > 1:451569/1‑71 (MQ=255) ttCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctg < 1:3548315/68‑1 (MQ=255) tCACTGTATTGCCATCTTCCAGTTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGtctgtctg > 1:1836292/1‑71 (MQ=255) tCACTGTATTGACATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTa > 1:1174001/1‑49 (MQ=255) cACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTa > 1:2609270/1‑48 (MQ=255) cACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTctgtctgt > 1:3987804/1‑71 (MQ=255) cACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTctgtctgt > 1:2846190/1‑71 (MQ=255) aCTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTg > 1:4201991/1‑60 (MQ=255) tATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGtt < 1:284345/70‑1 (MQ=255) tATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGtt < 1:4174818/70‑1 (MQ=255) aTTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTa > 1:3087324/1‑70 (MQ=255) ttGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTAt < 1:3944301/70‑1 (MQ=255) ttGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATc > 1:2471506/1‑71 (MQ=255) gCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGtt < 1:2736415/66‑1 (MQ=255) gCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCt < 1:4108917/70‑1 (MQ=255) cATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTAt > 1:1347052/1‑66 (MQ=255) cATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCtt > 1:3921157/1‑69 (MQ=255) aTCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTAt < 1:3921078/65‑1 (MQ=255) tCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCa < 1:4236001/43‑1 (MQ=255) tCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTg < 1:837752/48‑1 (MQ=255) tCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGgtctgt > 1:775225/1‑54 (MQ=255) cTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACg > 1:2192045/1‑71 (MQ=255) ttCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCttt < 1:1428335/66‑1 (MQ=255) ttCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGt > 1:3722340/1‑71 (MQ=255) ttCAAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCtgtctgtc > 1:436747/1‑57 (MQ=255) ccAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAAc < 1:3291414/67‑1 (MQ=255) ccAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGt < 1:3335257/69‑1 (MQ=255) ccAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTcc > 1:1032016/1‑71 (MQ=255) ccAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTcc > 1:1536568/1‑71 (MQ=255) cAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCt < 1:440742/71‑1 (MQ=255) gCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCt > 1:2444103/1‑69 (MQ=255) cTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGGTTTGTCTGTCGTTATCTTTAACGTCCTggg > 1:1050781/1‑71 (MQ=255) cTGCAACTGTTGCGGTTGTGCCCAGTACTGCTGCAACGTGGTTTGTCTGTCGTTATCTTTAACGTCCTggg > 1:1878187/1‑71 (MQ=255) cAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGt < 1:1109840/69‑1 (MQ=255) cAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGa < 1:2269015/71‑1 (MQ=255) cTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTaa > 1:1709015/1‑56 (MQ=255) tgttgtGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCttt < 1:3404429/53‑1 (MQ=255) ttgtGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTgg < 1:3648563/71‑1 (MQ=255) ttgtGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAAAGTCCTGGGTGAGGCTgg > 1:3518953/1‑71 (MQ=255) gtgGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGc < 1:4037224/70‑1 (MQ=255) gtgGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGc < 1:3850624/70‑1 (MQ=255) gtgGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGc < 1:3275284/70‑1 (MQ=255) gtgGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGc > 1:1981047/1‑70 (MQ=255) tgGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCttt < 1:3480868/48‑1 (MQ=255) tgGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCaa < 1:4256991/71‑1 (MQ=255) | GATTAAAACAGTGCTGGGCATCGCGTAACTGCCACTTCACTGTATTGCCATCTTCCAGCTGCAACTGTTGTGGCTGTGCCCAGTACTGCTGCAACGTGGTCTGTCTGTCGTTATCTTTAACGTCCTGGGTGAGGCTGGCAA > W3110S.gb/4177179‑4177319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |