| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,190,513 | G→A | 7.4% | R110H (CGC→CAC) | rplV → | 50S ribosomal subunit protein L22 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,190,513 | 0 | G | A | 7.4% | 201.8 / 4.6 | 67 | R110H (CGC→CAC) | rplV | 50S ribosomal subunit protein L22 |
| Reads supporting (aligned to +/- strand): ref base G (28/34); new base A (2/3); total (30/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
CCGCGTGCAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTGGGTATTGTAAAA > W3110S.gb/4190443‑4190582 | ccGCGTGCAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCg < 1:2747318/71‑1 (MQ=255) gcgTGCAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCt < 1:1605797/71‑1 (MQ=255) gTGCAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTga < 1:1529326/71‑1 (MQ=255) gTGCAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGACCGATCGCTga < 1:1769170/71‑1 (MQ=255) gTGCAAAAGGTCGTGCAGATCGCATCATGAAGCGCACCAGCCACATTACTGTGGTTGTGTCCGATCGCt > 1:1768312/1‑69 (MQ=255) cAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGAc > 1:228604/1‑71 (MQ=255) cAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGAc > 1:4035963/1‑71 (MQ=255) aaaaGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCt < 1:120336/65‑1 (MQ=255) aaaaGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCt < 1:1277769/65‑1 (MQ=255) aaGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGActct < 1:3963400/71‑1 (MQ=255) ggTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTg > 1:279549/1‑70 (MQ=255) tGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACt < 1:2516779/71‑1 (MQ=255) gCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGAc > 1:3065916/1‑69 (MQ=255) cAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAg > 1:3140952/1‑71 (MQ=255) aGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGc < 1:1572724/71‑1 (MQ=255) aGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGc < 1:2455920/71‑1 (MQ=255) aGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGc < 1:3673914/71‑1 (MQ=255) tCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAAt < 1:3988969/71‑1 (MQ=255) tCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAAt < 1:617552/71‑1 (MQ=255) tCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAAt < 1:3587926/71‑1 (MQ=255) gCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCTATCGCTGAGACTCTGGAGACTAGCAATgg > 1:1316576/1‑71 (MQ=255) gCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATgg < 1:1857880/71‑1 (MQ=255) gCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATgg < 1:3474956/71‑1 (MQ=255) gCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATgg < 1:1852484/71‑1 (MQ=255) aTCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGt < 1:402283/71‑1 (MQ=255) aTCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGt > 1:393959/1‑71 (MQ=255) aTCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGt < 1:2524632/71‑1 (MQ=255) aTCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGt > 1:1479470/1‑71 (MQ=255) cTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAg < 1:2367803/71‑1 (MQ=255) aaGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGa > 1:3817823/1‑69 (MQ=255) cACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGga > 1:3367817/1‑46 (MQ=255) ccAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGActct > 1:4019410/1‑41 (MQ=255) cAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATcc > 1:953643/1‑71 (MQ=255) aGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGaa > 1:2398930/1‑61 (MQ=255) aGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCt < 1:3811792/71‑1 (MQ=255) aGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCt < 1:3598282/71‑1 (MQ=255) gCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATcgt > 1:3746415/1‑68 (MQ=255) ccACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTaa < 1:1117747/71‑1 (MQ=255) cacaTCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTaa < 1:2299931/70‑1 (MQ=255) aTCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAg > 1:4022114/1‑57 (MQ=255) tCACTGTGGTTGTGTCCGATCGCTGAGACTCTGga < 1:2448703/35‑1 (MQ=255) tCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGaaa > 1:3344094/1‑55 (MQ=255) cACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGt < 1:2780688/69‑1 (MQ=255) cACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTAt > 1:4091932/1‑71 (MQ=255) cACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTAt > 1:1709400/1‑71 (MQ=255) aCTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTc > 1:742788/1‑48 (MQ=255) aCTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTAtt < 1:4334989/71‑1 (MQ=255) aCTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTAtt > 1:2046357/1‑71 (MQ=255) aCTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTAtt > 1:1184158/1‑71 (MQ=255) cTGTGGTTGTGTCCGATCACTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTaa < 1:1914200/63‑1 (MQ=255) cTGTGGTTGTGTCCGATCACTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTaa < 1:686442/63‑1 (MQ=255) cTGTGGTTGTGTCCGATCACTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTaa < 1:1736662/63‑1 (MQ=255) tgtgGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTa < 1:3023585/54‑1 (MQ=255) tgtgGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATg < 1:4430994/64‑1 (MQ=255) tgtgGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTa > 1:1809835/1‑67 (MQ=255) tgtgGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTa > 1:84381/1‑67 (MQ=255) gtgGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTaa < 1:1891959/61‑1 (MQ=255) gtgGTTGTGTCCGATCACTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATg > 1:4018017/1‑63 (MQ=255) gtgGTTGTGTCCGATCACTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATg > 1:3223786/1‑63 (MQ=255) tgGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAg < 1:579331/50‑1 (MQ=255) ttGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATcc < 1:4042671/54‑1 (MQ=255) tgtgTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTggg > 1:1628985/1‑71 (MQ=255) gtgtCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTggg < 1:361491/70‑1 (MQ=255) gATCGCTGAGACTCTGGAGACTAGCAATGGGTCAg < 1:208796/35‑1 (MQ=255) gATCGCTGAGACTCTGGAGACTAGCAATGGGTCAg < 1:1975396/35‑1 (MQ=255) tCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTGGGTAt > 1:3122361/1‑65 (MQ=255) cGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTGGGTATTGTaaaa > 1:4032444/1‑71 (MQ=255) cGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTGGGTATTGTaaaa > 1:983990/1‑71 (MQ=255) gCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTa > 1:1744319/1‑50 (MQ=255) | CCGCGTGCAAAAGGTCGTGCAGATCGCATCCTGAAGCGCACCAGCCACATCACTGTGGTTGTGTCCGATCGCTGAGACTCTGGAGACTAGCAATGGGTCAGAAAGTACATCCTAATGGTATTCGCCTGGGTATTGTAAAA > W3110S.gb/4190443‑4190582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |