| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,218,735 | G→T | 8.5% | W288C (TGG→TGT) | metA → | homoserine transsuccinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,218,735 | 0 | G | T | 8.5% | 176.9 / 2.2 | 58 | W288C (TGG→TGT) | metA | homoserine transsuccinylase |
| Reads supporting (aligned to +/- strand): ref base G (33/20); new base T (3/2); total (36/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCT > W3110S.gb/4218666‑4218804 | gCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGc > 1:261384/1‑71 (MQ=255)gCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGc < 1:241698/71‑1 (MQ=255)gCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGc > 1:4217823/1‑71 (MQ=255) caATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCa < 1:2572589/71‑1 (MQ=255) caATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCa < 1:2206360/71‑1 (MQ=255) aTGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAAc < 1:2283861/71‑1 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGTCTCAACTAt > 1:1063701/1‑71 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTAt > 1:3379495/1‑71 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTAt < 1:1081655/71‑1 (MQ=255) aTCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTAt < 1:3651373/71‑1 (MQ=255) cGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTAc > 1:2393546/1‑71 (MQ=255) cAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGTCTCaa < 1:3440631/62‑1 (MQ=255) aaaaTACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc < 1:3234193/71‑1 (MQ=255) cacCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCaa > 1:4161440/1‑55 (MQ=255) cgcgAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTc < 1:2609131/50‑1 (MQ=255) cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc > 1:3485904/1‑60 (MQ=255) cgagcgagCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc > 1:119013/1‑60 (MQ=255) gagcgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACg < 1:1420597/71‑1 (MQ=255) cgagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCa < 1:2618298/71‑1 (MQ=255) gCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATc < 1:3940113/71‑1 (MQ=255) gCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATc > 1:4067610/1‑71 (MQ=255) gTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACgg > 1:97013/1‑71 (MQ=255) gTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACgg > 1:2450181/1‑71 (MQ=255) tCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACg < 1:1282160/62‑1 (MQ=255) aCGGTAATTTACTGTTTACCAACTGTCTCAACTAt > 1:980830/1‑35 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTAt > 1:3489381/1‑35 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTc > 1:4424816/1‑41 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:2413148/1‑70 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:2125228/1‑70 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:3614661/1‑70 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:3187272/1‑70 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:1829157/1‑70 (MQ=255) aCGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca > 1:1790897/1‑70 (MQ=255) gTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATg < 1:2026043/71‑1 (MQ=255) tAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATc > 1:2017424/1‑46 (MQ=255) tAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATg > 1:3917280/1‑70 (MQ=255) tAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa < 1:3908763/71‑1 (MQ=255) tttACTGTTTACCAACTGGCTCAACTATTACGTCt < 1:1589024/35‑1 (MQ=255) tttACTGTTTACCAACTGGCTCAACTATTACGTCTACCa < 1:1697136/39‑1 (MQ=255) cTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAAc > 1:3108999/1‑71 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATc > 1:2781614/1‑36 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAAc < 1:3435516/68‑1 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGc < 1:1622104/70‑1 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCt > 1:1309539/1‑71 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAAAGCt > 1:407455/1‑71 (MQ=255) tttACCAACTGGCTCAACTATTACGTCTACAAGATCACGCCATACGATCTACGGCACATGAATCCAAc < 1:1580752/68‑1 (MQ=255) ttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg > 1:3765738/1‑71 (MQ=255) ttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg > 1:2433254/1‑71 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa > 1:57395/1‑59 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGa > 1:377629/1‑59 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTg < 1:2866237/70‑1 (MQ=255) tACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGATg > 1:3932016/1‑70 (MQ=255) ccAACTGTCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGa < 1:2910611/70‑1 (MQ=255) ccAACTGTCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGAt > 1:1710811/1‑71 (MQ=255) aaCTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGAt > 1:1591318/1‑69 (MQ=255) aCTGGCTCAACTATTACGTCTACCAGATCACGCCATa > 1:311682/1‑37 (MQ=255) aCTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTa > 1:1366768/1‑44 (MQ=255) aCTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTa > 1:287286/1‑70 (MQ=255) gCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGca < 1:1080957/45‑1 (MQ=255) gCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAAtct < 1:3008053/70‑1 (MQ=255) | GCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCT > W3110S.gb/4218666‑4218804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |