| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,267,116 | G→C | 7.0% | V236V (GTC→GTG) | qor ← | quinone oxidoreductase, NADPH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,267,116 | 0 | G | C | 7.0% | 159.9 / 2.0 | 57 | V236V (GTC→GTG) | qor | quinone oxidoreductase, NADPH‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (20/32); new base C (2/2); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AACGAGCCTTTTTGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACA > W3110S.gb/4267048‑4267186 | aaCGAGCCTTTTTGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGAc < 1:3900355/71‑1 (MQ=255) aCGAGCCTTTTTGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGAc > 1:318935/1‑70 (MQ=255) aCGAGCCTTTTTGATTGAGAATGCCTAAGTTCACACCAGTAACCGCACCTGATGAGTTGCCAAAACTGAc > 1:120167/1‑70 (MQ=255) aGCCTTTTTGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCAtt < 1:3809543/71‑1 (MQ=255) ccTTTTTGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGAcc < 1:3164/66‑1 (MQ=255) tttGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGc > 1:856152/1‑69 (MQ=255) ttgattgaGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGcc > 1:2645060/1‑69 (MQ=255) ttgattgaGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCcgc > 1:2988674/1‑71 (MQ=255) ttgattgaGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCcgc > 1:3738911/1‑71 (MQ=255) ttgattgaGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCcgc > 1:1698503/1‑71 (MQ=255) attgaGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGAc < 1:749342/57‑1 (MQ=255) agaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTg < 1:497100/71‑1 (MQ=255) gaATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTg < 1:1613341/70‑1 (MQ=255) tGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCcgg > 1:2222515/1‑59 (MQ=255) tGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGtt < 1:1661813/66‑1 (MQ=255) tAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGc < 1:1575356/64‑1 (MQ=255) tAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGc < 1:1082203/64‑1 (MQ=255) aaGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTTACCATTAAGCCGCGGCGTTGCAGGCAATc > 1:3516420/1‑71 (MQ=255) aGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATc < 1:2977048/70‑1 (MQ=255) ttCACACCGGTAACCGCACCTGATGAGTTCCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAg < 1:1067695/71‑1 (MQ=255) cacaCCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAg > 1:1753439/1‑60 (MQ=255) cacaCCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGc < 1:2086871/70‑1 (MQ=255) ggTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAg > 1:3474551/1‑40 (MQ=255) gTAACCGCACCTGATGAGTTGCCAAAACTCACCATTAAGCCGCGGCGTTGCAGGCAATCCAGc > 1:1003902/1‑63 (MQ=255) gTAACCGCACCTGATGAGTTGCCAAAACTCACCATTAAGCCGCGGCGTTGCAGGCAATCCAGc > 1:2634261/1‑63 (MQ=255) tAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTcc < 1:2466235/71‑1 (MQ=255) tAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTc < 1:3351638/70‑1 (MQ=255) aaCCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTcc < 1:2471141/70‑1 (MQ=255) aaCCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTcc > 1:2555851/1‑70 (MQ=255) aCCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTccc > 1:3037898/1‑70 (MQ=255) aCCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGgtgt < 1:1668353/71‑1 (MQ=255) cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTg < 1:1221528/40‑1 (MQ=255) cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTg < 1:2976042/40‑1 (MQ=255) cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGt < 1:1169531/59‑1 (MQ=255) cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGtct < 1:2158857/71‑1 (MQ=255) cTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGtct > 1:1557471/1‑71 (MQ=255) tgatgaGTTGCCAAAACTGACCATTAAGCCgcggc < 1:2641406/35‑1 (MQ=255) atgaGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTg > 1:3297683/1‑71 (MQ=255) tgaGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGt > 1:1051862/1‑55 (MQ=255) tgaGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGt > 1:4347498/1‑55 (MQ=255) aGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGcc < 1:1608131/70‑1 (MQ=255) gTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTcc < 1:2745450/55‑1 (MQ=255) gTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTcc < 1:1002847/55‑1 (MQ=255) ccAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACgg < 1:656710/70‑1 (MQ=255) ccAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGa < 1:1688595/71‑1 (MQ=255) cAAAACTCACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTGCCAg < 1:3988228/53‑1 (MQ=39) cAAAACTCACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAg < 1:2147906/53‑1 (MQ=255) aaaaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGgt < 1:2780400/54‑1 (MQ=255) aaaaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGgt < 1:3491752/54‑1 (MQ=255) aaaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGgtgt > 1:2000377/1‑55 (MQ=255) aaaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATc > 1:3502498/1‑71 (MQ=255) aaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCa > 1:2538134/1‑36 (MQ=255) aaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCg < 1:3700921/71‑1 (MQ=255) aaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCg < 1:2368341/71‑1 (MQ=255) aaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCg < 1:1505449/71‑1 (MQ=255) aaCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCg < 1:988593/71‑1 (MQ=255) aCTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCg > 1:733928/1‑70 (MQ=255) gACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTaca > 1:548198/1‑71 (MQ=255) gACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTaca > 1:56200/1‑71 (MQ=255) | AACGAGCCTTTTTGATTGAGAATGCCTAAGTTCACACCGGTAACCGCACCTGATGAGTTGCCAAAACTGACCATTAAGCCGCGGCGTTGCAGGCAATCCAGCGACCGTTCCCAGGTGTCTCTGCCCACGGAATCGTACA > W3110S.gb/4267048‑4267186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |