Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 85,767 A→T 7.5% V46V (GTA→GTT)  ilvI → acetolactate synthase III, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb85,7670AT 7.5% 137.4 / 2.6 53V46V (GTA→GTT) ilvIacetolactate synthase III, large subunit
Reads supporting (aligned to +/- strand):  ref base A (27/22);  new base T (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTATCCCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAG  >  W3110S.gb/85699‑85831
                                                                    |                                                                
ggTTATCCCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTAt                                                                 <  1:4066058/70‑1 (MQ=255)
  ttATCCCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAg                                                              <  1:3205047/71‑1 (MQ=255)
    aTCCCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTa                                                               <  1:342321/68‑1 (MQ=255)
       ccGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGt                                                         <  1:1204677/71‑1 (MQ=255)
        cGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTc                                                        <  1:4395094/71‑1 (MQ=255)
          gAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAg                                                              >  1:1932631/1‑63 (MQ=255)
          gAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCAt                                                      <  1:1302157/71‑1 (MQ=255)
          gAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCAt                                                      <  1:4123373/71‑1 (MQ=255)
           aGGCGCGGTCCTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGCCATg                                                     <  1:2235003/71‑1 (MQ=255)
           aGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATg                                                     <  1:3900002/71‑1 (MQ=255)
           aGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATg                                                     <  1:4115921/71‑1 (MQ=255)
                 aGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAg                                                              >  1:2361217/1‑56 (MQ=255)
                   tCCTTGATATTTATGATTCATTGCATACCGTGGGTGTTATTGATCATGTAt                                                                 <  1:1220472/51‑1 (MQ=255)
                    ccTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCa                                                 >  1:3306136/1‑66 (MQ=255)
                    ccTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGCCATg                                                     >  1:3225205/1‑62 (MQ=255)
                    ccTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGCCATg                                                     >  1:877746/1‑62 (MQ=255)
                     cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCa                                                 >  1:1031809/1‑65 (MQ=255)
                     cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                           >  1:3345222/1‑71 (MQ=255)
                     cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                           >  1:3221665/1‑71 (MQ=255)
                     cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                           <  1:2656574/71‑1 (MQ=255)
                     cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                           >  1:1468953/1‑71 (MQ=255)
                     cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                           >  1:2982095/1‑71 (MQ=255)
                        gATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTa                                                               >  1:2997429/1‑48 (MQ=255)
                        gATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGt                                        >  1:1759156/1‑71 (MQ=255)
                        gATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGt                                        >  1:3225165/1‑71 (MQ=255)
                          tatTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTg                                       <  1:1709573/70‑1 (MQ=255)
                            tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                     <  1:3898458/70‑1 (MQ=255)
                            tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                     <  1:3389855/70‑1 (MQ=255)
                            tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                     <  1:4330777/70‑1 (MQ=255)
                            tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                     <  1:1804658/70‑1 (MQ=255)
                              tattatGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGc                                      >  1:3711647/1‑67 (MQ=255)
                              tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGc                                                  >  1:3269136/1‑55 (MQ=255)
                              tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCatat                                  >  1:3642642/1‑71 (MQ=255)
                              tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCata                                   <  1:1758617/70‑1 (MQ=255)
                              tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCata                                   >  1:998776/1‑70 (MQ=255)
                               atgatgCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATg                                 >  1:4359422/1‑71 (MQ=255)
                                      attgcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                         >  1:915022/1‑56 (MQ=255)
                                        tgcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCa                                                       >  1:3087314/1‑40 (MQ=255)
                                         gcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCAt                                                      >  1:1267655/1‑40 (MQ=255)
                                         gcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCa                                                 >  1:2670352/1‑45 (MQ=255)
                                         gcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGcc                       >  1:39135/1‑71 (MQ=255)
                                          catACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGcc                       <  1:3882250/70‑1 (MQ=255)
                                              ccGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCata                                   >  1:108151/1‑54 (MQ=255)
                                               cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                         >  1:3456977/1‑47 (MQ=255)
                                               cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                         >  1:2998024/1‑47 (MQ=255)
                                               cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                         >  1:2038020/1‑47 (MQ=255)
                                                 tgggtggTATTGATGATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGcc                              <  1:1898145/56‑1 (MQ=255)
                                                 tgggtggTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCTGTGCATATGGCCGATGGcc                       <  1:1528887/63‑1 (MQ=255)
                                                 tgggtggTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGcc                              <  1:1098434/56‑1 (MQ=255)
                                                        tATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                     <  1:3067843/42‑1 (MQ=255)
                                                          ttGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCg                             >  1:599602/1‑48 (MQ=255)
                                                             aTCATGTTTTAGTTCGCCATGAGCAGGCGGCGGTGCATATGGCCGATggtc                       <  1:1449114/51‑3 (MQ=38)
                                                              tCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAg  >  1:1490405/1‑71 (MQ=255)
                                                                    |                                                                
GGTTATCCCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAG  >  W3110S.gb/85699‑85831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑