Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 85,776 T→C 7.8% R49R (CGT→CGC ilvI → acetolactate synthase III, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb85,7760TC 7.8% 129.3 / 2.7 51R49R (CGT→CGCilvIacetolactate synthase III, large subunit
Reads supporting (aligned to +/- strand):  ref base T (27/20);  new base C (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCT  >  W3110S.gb/85706‑85844
                                                                      |                                                                    
ccGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGt                                                                      <  1:1204677/71‑1 (MQ=255)
 cGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTc                                                                     <  1:4395094/71‑1 (MQ=255)
   gAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCAt                                                                   <  1:1302157/71‑1 (MQ=255)
   gAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCAt                                                                   <  1:4123373/71‑1 (MQ=255)
    aGGCGCGGTCCTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGCCATg                                                                  <  1:2235003/71‑1 (MQ=255)
    aGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATg                                                                  <  1:3900002/71‑1 (MQ=255)
    aGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATg                                                                  <  1:4115921/71‑1 (MQ=255)
             ccTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCa                                                              >  1:3306136/1‑66 (MQ=255)
             ccTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGCCATg                                                                  >  1:3225205/1‑62 (MQ=255)
             ccTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGCCATg                                                                  >  1:877746/1‑62 (MQ=255)
              cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCa                                                              >  1:1031809/1‑65 (MQ=255)
              cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                                        <  1:2656574/71‑1 (MQ=255)
              cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                                        >  1:3221665/1‑71 (MQ=255)
              cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                                        >  1:3345222/1‑71 (MQ=255)
              cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                                        >  1:2982095/1‑71 (MQ=255)
              cTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAggcggc                                                        >  1:1468953/1‑71 (MQ=255)
                 gATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGt                                                     >  1:1759156/1‑71 (MQ=255)
                 gATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGt                                                     >  1:3225165/1‑71 (MQ=255)
                   tatTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTg                                                    <  1:1709573/70‑1 (MQ=255)
                     tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                                  <  1:4330777/70‑1 (MQ=255)
                     tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                                  <  1:3389855/70‑1 (MQ=255)
                     tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                                  <  1:1804658/70‑1 (MQ=255)
                     tttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                                  <  1:3898458/70‑1 (MQ=255)
                       tattatGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGc                                                   >  1:3711647/1‑67 (MQ=255)
                       tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGc                                                               >  1:3269136/1‑55 (MQ=255)
                       tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCatat                                               >  1:3642642/1‑71 (MQ=255)
                       tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCata                                                <  1:1758617/70‑1 (MQ=255)
                       tATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCata                                                >  1:998776/1‑70 (MQ=255)
                        atgatgCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATg                                              >  1:4359422/1‑71 (MQ=255)
                               attgcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                                      >  1:915022/1‑56 (MQ=255)
                                 tgcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCa                                                                    >  1:3087314/1‑40 (MQ=255)
                                  gcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCAt                                                                   >  1:1267655/1‑40 (MQ=255)
                                  gcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCa                                                              >  1:2670352/1‑45 (MQ=255)
                                  gcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGcc                                    >  1:39135/1‑71 (MQ=255)
                                   catACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGcc                                    <  1:3882250/70‑1 (MQ=255)
                                       ccGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCata                                                >  1:108151/1‑54 (MQ=255)
                                        cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                                      >  1:3456977/1‑47 (MQ=255)
                                        cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                                      >  1:2998024/1‑47 (MQ=255)
                                        cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGcggcgg                                                      >  1:2038020/1‑47 (MQ=255)
                                          tgggtggTATTGATGATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGcc                                           <  1:1898145/56‑1 (MQ=255)
                                          tgggtggTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCTGTGCATATGGCCGATGGcc                                    <  1:1528887/63‑1 (MQ=255)
                                          tgggtggTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGcc                                           <  1:1098434/56‑1 (MQ=255)
                                                 tATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCa                                                  <  1:3067843/42‑1 (MQ=255)
                                                   ttGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCg                                          >  1:599602/1‑48 (MQ=255)
                                                      aTCATGTTTTAGTTCGCCATGAGCAGGCGGCGGTGCATATGGCCGATggtc                                    <  1:1449114/51‑3 (MQ=38)
                                                       tCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAg               >  1:1490405/1‑71 (MQ=255)
                                                                aGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGaa                >  1:3106513/1‑61 (MQ=255)
                                                                 gTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGcctcgt     <  1:1954626/71‑1 (MQ=255)
                                                                  ttCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGcgtcgt     <  1:3493608/70‑1 (MQ=255)
                                                                    cGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCt                                   >  1:75252/1‑38 (MQ=255)
                                                                    cGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCt  >  1:3003670/1‑71 (MQ=255)
                                                                     gTCATGAGCAGGCGGCGGCGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGc   <  1:1144378/69‑1 (MQ=255)
                                                                      |                                                                    
CCGGAGGCGCAGTCCTTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCT  >  W3110S.gb/85706‑85844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: