| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 188,973 | A→G | 9.5% | H178H (CAT→CAC) | map ← | methionine aminopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 188,973 | 0 | A | G | 9.5% | 105.6 / 2.8 | 42 | H178H (CAT→CAC) | map | methionine aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base A (22/16); new base G (2/2); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATT > W3110S.gb/188904‑189043 | gAACGTCATCCCAGGTTTCAGTACGACGTTGATTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt > 1:410522/1‑71 (MQ=255) aaCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt < 1:1278238/70‑1 (MQ=255) cGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATgg > 1:2498770/1‑70 (MQ=255) aTCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGcc > 1:2677885/1‑71 (MQ=255) tCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcg > 1:4377711/1‑71 (MQ=255) ccAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc > 1:4304527/1‑70 (MQ=255) ccAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgc > 1:2862042/1‑70 (MQ=255) aGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAc < 1:2723697/71‑1 (MQ=255) ggTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAc > 1:3942518/1‑70 (MQ=255) gTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCa < 1:670026/71‑1 (MQ=255) tCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa > 1:1612814/1‑53 (MQ=255) tCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAt < 1:1105876/70‑1 (MQ=255) agcacgacgTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCGTGGAAGCCGCGACCAATAc < 1:4151466/67‑1 (MQ=255) agcacgacgTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCGTGGAAGCCGCGACCAATAc < 1:96383/67‑1 (MQ=255) aGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATTGAAGCCGCGACCAATAc < 1:2021254/70‑1 (MQ=255) aGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATAcc < 1:3169305/71‑1 (MQ=255) aGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATAcc < 1:883407/71‑1 (MQ=255) gTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATAcc < 1:4052533/70‑1 (MQ=255) acgacgTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg > 1:2509009/1‑71 (MQ=255) acgacgTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtg > 1:1528832/1‑71 (MQ=255) cgacgTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGa > 1:3289502/1‑51 (MQ=255) tGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCaa > 1:3853497/1‑57 (MQ=255) tGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCaa > 1:4030788/1‑57 (MQ=255) tGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCaa > 1:492095/1‑71 (MQ=255) ggTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAg > 1:1738988/1‑46 (MQ=255) gTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAcc < 1:1324647/53‑1 (MQ=255) tCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATAtt > 1:197062/1‑70 (MQ=255) gAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac < 1:1253754/71‑1 (MQ=255) gAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac < 1:813085/71‑1 (MQ=255) aTAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCTCAATATTCACGAaccacg < 1:3240072/70‑1 (MQ=255) aTAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgacg > 1:2880832/1‑70 (MQ=255) tAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga < 1:3696076/67‑1 (MQ=255) gTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATAcc > 1:3448052/1‑42 (MQ=255) gcagcaCCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAat > 1:433324/1‑51 (MQ=255) ccTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCt < 1:3752883/71‑1 (MQ=255) tGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgaga > 1:2717223/1‑61 (MQ=255) cGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCt < 1:2149343/70‑1 (MQ=255) ggTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTc < 1:1521052/71‑1 (MQ=255) gTTCTTCGTGGAAGCGGCGACCAATACCGTGTCCACAatat > 1:1338174/1‑41 (MQ=25) gTTCTTCGTGGAAGCCGCGACCAATACCGTGTCCACAATAtt > 1:2272180/1‑42 (MQ=38) ttcATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc > 1:1182695/1‑56 (MQ=255) tcATGGAAGCCGCGACCAATACCGTGTCCGCAata > 1:4431492/1‑35 (MQ=255) cATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCac > 1:75329/1‑39 (MQ=255) aTGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAtt > 1:171557/1‑71 (MQ=255) aTGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAt < 1:3962038/70‑1 (MQ=255) | GAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATT > W3110S.gb/188904‑189043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |