| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,101 | A→C | 91.4% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,101 | 0 | A | C | 91.4% | 2696.1 / 104.4 | 851 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (43/31); new base C (434/342); total (477/374) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
Only 100 of 853 total aligned reads displayed.
AACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGC > W3110S.gb/236031‑236170 | cgcgtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTa > 1:3795848‑M2/12‑71 (MQ=255) gTAAAACGCCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAcc > 1:3243706/1‑71 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:697835/70‑1 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGGGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:2557175‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAccgca > 1:2943515‑M2/6‑68 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1359744‑M2/6‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:4318873/1‑71 (MQ=255) actCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:593045‑M2/4‑70 (MQ=255) ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:752109‑M2/3‑71 (MQ=255) tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacc < 1:806208‑M2/68‑1 (MQ=255) tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:1403242‑M2/67‑1 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:447682/1‑71 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:3034730/1‑71 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:3905548/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg < 1:183187/71‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:200848/1‑71 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:3389124/71‑1 (MQ=255) gTCTGACATAAATGGCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAACGCAACCACCgg > 1:4017488/1‑70 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGt < 1:2092922/71‑1 (MQ=255) cTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:1878984/1‑71 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:3707590/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:335247/1‑70 (MQ=255) gACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg > 1:2119970/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:1783895/69‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:2392119/69‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:4233710/69‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:1446583/70‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:2987399/62‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1908260/1‑68 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:3457299/1‑68 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:4268316/1‑68 (MQ=255) aaTGCCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTa > 1:3184634/1‑47 (MQ=39) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:2652075/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:129217/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:3972051/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:3624505/1‑67 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt > 1:2277603/1‑71 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACAGGTATGAACCAGAtt > 1:224197/1‑71 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg < 1:1938431/71‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAc > 1:2907190/1‑40 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:3551667/1‑46 (MQ=255) tATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:958940/51‑1 (MQ=255) aTGAGCACTGCAATTCCACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa > 1:486144/1‑52 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa > 1:363903/1‑52 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:3867117/1‑71 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcgc < 1:1977401/69‑1 (MQ=255) aGCACTGCAATTACACGCGAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt > 1:2434618/1‑59 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTAc > 1:2878386/1‑35 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:3756181/1‑71 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:3216362/1‑71 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:351435/1‑46 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAATCACCgg < 1:5378/45‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2351800/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:83480/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2719698/71‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa < 1:2199474/64‑1 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:3282329/47‑1 (MQ=39) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:1626760/55‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt > 1:406717/1‑54 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGt > 1:1257273/1‑57 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:4092993/1‑66 (MQ=255) gCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:3085919/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:3832311/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:2528580/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:1568356/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:1310837/1‑71 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg < 1:1136340/52‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:4409488/71‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:1494238/1‑47 (MQ=39) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTa > 1:1518779/1‑37 (MQ=37) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:2592729/1‑56 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2825882/1‑69 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:3431645/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:1209321/71‑1 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:2477987/44‑1 (MQ=38) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:3684781/70‑1 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt > 1:918591/1‑70 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:2623142/71‑1 (MQ=255) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3309696/55‑1 (MQ=255) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:2757313/63‑1 (MQ=255) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt > 1:666914/1‑71 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:2680789/36‑1 (MQ=37) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1115010/1‑70 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:3927335/66‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:4215188/71‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:2167409/71‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:4130880/1‑68 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1660115/1‑68 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:357447/1‑68 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATT‑GTGCGCACTATGAAGGCCACAAGATCAtt > 1:1167514/1‑67 (MQ=255) tCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:1697250/47‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa > 1:1022279/1‑65 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:4166141/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:315177/1‑55 (MQ=255) gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:1002559/71‑1 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt < 1:876242/71‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga > 1:175397/1‑67 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:1066896/1‑71 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg > 1:4353533/1‑70 (MQ=255) ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:2037194/1‑48 (MQ=255) | AACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGC > W3110S.gb/236031‑236170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |