Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 236,107 A→C 91.7% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb236,1070AC91.7% 2608.5 / 97.6 823E14A (GAA→GCA) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base A (39/30);  new base C (397/356);  total (437/386)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 860 total aligned reads displayed.

AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGA  >  W3110S.gb/236038‑236176
                                                                     |                                                                     
aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa                                                                       <  1:697835/70‑1 (MQ=255)
aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa                                                                       <  1:1169724/70‑1 (MQ=255)
 caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa                                                                     >  1:4363943‑M2/6‑71 (MQ=255)
 caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa                                                                     >  1:382881‑M2/6‑71 (MQ=255)
    ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca                                                                  >  1:2603559‑M2/3‑71 (MQ=255)
     tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacc                                                                   <  1:806208‑M2/68‑1 (MQ=255)
     tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac                                                                    <  1:1403242‑M2/67‑1 (MQ=255)
      cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc                                                                >  1:965783/1‑71 (MQ=255)
      cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc                                                                >  1:474393/1‑71 (MQ=255)
      cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc                                                                >  1:1354488/1‑71 (MQ=255)
       aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg                                                               >  1:2061093/1‑71 (MQ=255)
        aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                              <  1:3389124/71‑1 (MQ=255)
         gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                              >  1:3430041/1‑70 (MQ=255)
         gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGt                                                             <  1:418816/71‑1 (MQ=255)
              aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa                                                         <  1:606091/70‑1 (MQ=255)
                aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAcc                                                      <  1:1665203/71‑1 (MQ=255)
                 tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa                                                     <  1:2187480/71‑1 (MQ=255)
                  aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                       >  1:680646/1‑68 (MQ=255)
                   aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCTGTATGAAc                                                       >  1:252873/1‑67 (MQ=255)
                   aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                       >  1:1111129/1‑67 (MQ=255)
                   aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                       >  1:2262102/1‑67 (MQ=255)
                    aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa                                                     <  1:2406777/68‑1 (MQ=255)
                     tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt                                                 <  1:1886200/71‑1 (MQ=255)
                      gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAAGAACCAGATTg                                                >  1:2802127/1‑71 (MQ=255)
                       aCCGCTATGAGCACTGCAATTACACGCCAGATCTTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                                <  1:2437437/70‑1 (MQ=255)
                       aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                                <  1:3890133/70‑1 (MQ=255)
                       aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                                <  1:548546/70‑1 (MQ=255)
                            tATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg                                                          <  1:407167/55‑1 (MQ=255)
                            tATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAg                                                    <  1:3706647/61‑1 (MQ=255)
                             aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa                                                            >  1:1582939/1‑52 (MQ=255)
                             aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa                                         >  1:2930507/1‑71 (MQ=255)
                              tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc                                        <  1:3554396/71‑1 (MQ=255)
                                aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa                                      >  1:1287940/1‑71 (MQ=255)
                                aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGAGCACTa                                      >  1:1916254/1‑71 (MQ=255)
                                aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGaa                                                        >  1:2859645/1‑53 (MQ=255)
                                 gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                              >  1:351435/1‑46 (MQ=255)
                                 gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt                                     <  1:2590604/71‑1 (MQ=255)
                                  cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                    <  1:83480/71‑1 (MQ=255)
                                  cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                    <  1:3350802/71‑1 (MQ=255)
                                   aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                    <  1:2712077/70‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                              <  1:1710784/42‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa                                                         <  1:2894758/47‑1 (MQ=39)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt                                                 <  1:3012822/55‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt                                                 <  1:1626760/55‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                    <  1:3232218/68‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg                                 >  1:3457001/1‑71 (MQ=255)
                                      gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGt                                              >  1:1257273/1‑57 (MQ=255)
                                      gCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg                                >  1:3085919/1‑71 (MQ=255)
                                       cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg                                 <  1:2134934/69‑1 (MQ=255)
                                       cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg                                 <  1:3270984/69‑1 (MQ=255)
                                       cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc                               >  1:1310837/1‑71 (MQ=255)
                                         aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca                             >  1:403413/1‑71 (MQ=255)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa                                                   <  1:4348503/48‑1 (MQ=39)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg                                               <  1:1136340/52‑1 (MQ=255)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                                <  1:2642861/51‑1 (MQ=255)
                                            acacTCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                            >  1:144985/1‑69 (MQ=255)
                                            acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcaca                           <  1:4148776/70‑1 (MQ=255)
                                            acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                            >  1:2215781/1‑69 (MQ=255)
                                            acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                            >  1:2105777/1‑69 (MQ=255)
                                            acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca                             >  1:3678387/1‑68 (MQ=255)
                                              acGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa                        <  1:2013015/71‑1 (MQ=255)
                                               cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                            >  1:3921892/1‑66 (MQ=255)
                                               cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg                         <  1:1984827/69‑1 (MQ=255)
                                               cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt                       <  1:1209321/71‑1 (MQ=255)
                                                gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt                       >  1:918591/1‑70 (MQ=255)
                                                gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt                       >  1:3960603/1‑70 (MQ=255)
                                                  cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt                    >  1:2305932/1‑71 (MQ=255)
                                                    gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcgc                                          <  1:1791343/47‑1 (MQ=39)
                                                    gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:4249157/1‑70 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAtt                                    <  1:528790/52‑2 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                    <  1:152351/52‑1 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   <  1:3773346/69‑1 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga                 <  1:4223425/71‑1 (MQ=255)
                                                     aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc                                        <  1:2751992/48‑1 (MQ=255)
                                                     aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                            <  1:3131478/60‑1 (MQ=255)
                                                      tCGTTCTCGCTACCGCAACCACCGGTATGACCCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:4286213/1‑68 (MQ=255)
                                                      tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:357447/1‑68 (MQ=255)
                                                      tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag                <  1:4101919/71‑1 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa                                  >  1:1057238/1‑52 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa                                   >  1:3638792/1‑51 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa                     >  1:1022279/1‑65 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:3740551/1‑67 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:1755893/1‑67 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:3209696/1‑67 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                   >  1:4425435/1‑67 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGACACAAGATCAtt                   >  1:184898/1‑67 (MQ=255)
                                                       cGTTCTCGATACCGAAACCACCGGTATGAACCAGa                                                   >  1:3180652/1‑35 (MQ=255)
                                                        gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt              <  1:1002559/71‑1 (MQ=255)
                                                         ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa                                         >  1:3858438/1‑43 (MQ=38)
                                                         ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt             <  1:3303840/71‑1 (MQ=255)
                                                         ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt             <  1:876242/71‑1 (MQ=255)
                                                         ttCTCGATACCGAAACCACCGGTATGAACCAGATTGGTgcgc                                          <  1:2496221/42‑1 (MQ=255)
                                                          tctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa                                  >  1:2979663/1‑49 (MQ=39)
                                                          tctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga               <  1:2665884/68‑1 (MQ=255)
                                                           ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACaa                          >  1:2363714/1‑56 (MQ=255)
                                                              gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt              >  1:1950118/3‑65 (MQ=255)
                                                              gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc        <  1:2558693/69‑1 (MQ=255)
                                                              gctACCGCAAACACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGt          <  1:3624468/67‑1 (MQ=255)
                                                                tACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt             <  1:758104/64‑1 (MQ=255)
                                                                tACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt             <  1:1502569/64‑1 (MQ=255)
                                                                     |                                                                     
AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGA  >  W3110S.gb/236038‑236176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: