| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 98.2% | 169.3 / ‑4.5 | 54 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (30/23); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAG > W3110S.gb/547629‑547758 | cgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa > 1:1066540/1‑70 (MQ=255) cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa < 1:1425343/68‑1 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:694392/71‑1 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:4332163/71‑1 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:3070827/71‑1 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCt > 1:1020480/1‑70 (MQ=255) tCCACACTGGCTGGATGTG‑AACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa < 1:2504466/58‑1 (MQ=255) acacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:2511527/57‑1 (MQ=255) acacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac > 1:2077924/1‑62 (MQ=255) cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:1772818/1‑58 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc < 1:4403188/71‑1 (MQ=255) aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCa > 1:932031/1‑58 (MQ=255) aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCa > 1:146131/1‑58 (MQ=255) aaCCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc < 1:3685361/71‑1 (MQ=255) ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:1437526/1‑70 (MQ=255) tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:527570/1‑69 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:2011279/70‑1 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:2404828/70‑1 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:2738101/1‑67 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:3202323/1‑67 (MQ=255) tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTc > 1:2821063/1‑40 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg > 1:1996678/1‑56 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg > 1:3508330/1‑56 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:2869253/1‑69 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag > 1:3816360/1‑68 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGGGATGa < 1:3511806/71‑1 (MQ=255) cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc > 1:1432348/1‑71 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag > 1:3105918/1‑65 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag > 1:1739274/1‑65 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:4255018/64‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:2956590/64‑1 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:1258035/1‑70 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:116137/1‑70 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATCACCg > 1:3962854/1‑70 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCgg < 1:165868/70‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc < 1:2658802/43‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc < 1:3818894/43‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc < 1:1758310/71‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc > 1:2129417/1‑71 (MQ=255) gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:2369819/1‑44 (MQ=255) gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:2364449/1‑44 (MQ=255) gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:3364247/1‑44 (MQ=255) gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGGGCTGGCAGGAGATGACCGGACCCAt > 1:2993446/1‑71 (MQ=255) aaCTAAACGCGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAt < 1:2524165/70‑1 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:2272784/58‑1 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:1249499/58‑1 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAt < 1:2529383/70‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:499434/38‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa > 1:2508444/1‑70 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:1550048/52‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:2621064/52‑1 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg > 1:3914298/1‑71 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:1303054/1‑37 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:661016/1‑37 (MQ=255) | CGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAG > W3110S.gb/547629‑547758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |