| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 651,898 | C→T | 8.6% | R147R (CGC→CGT) | citA → | sensory histidine kinase in two‑component regulatory system with citB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 651,898 | 0 | C | T | 8.6% | 204.9 / 5.8 | 69 | R147R (CGC→CGT) | citA | sensory histidine kinase in two‑component regulatory system with citB |
| Reads supporting (aligned to +/- strand): ref base C (27/36); new base T (2/4); total (29/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGG > W3110S.gb/651828‑651962 | aaCAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATgcgc > 1:4143098/1‑71 (MQ=255) ggAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa < 1:1158716/63‑1 (MQ=255) ggAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa < 1:4278288/63‑1 (MQ=255) gagaAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCaa > 1:2677812/1‑70 (MQ=255) gagaAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCa < 1:2613115/69‑1 (MQ=255) gagaAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAAt > 1:1392204/1‑71 (MQ=255) aaGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCttt < 1:1929513/71‑1 (MQ=255) gagaGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga > 1:3591208/1‑71 (MQ=255) gagaGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga > 1:1359440/1‑71 (MQ=255) gagaGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga < 1:1646040/71‑1 (MQ=255) agagCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGAc < 1:3328053/71‑1 (MQ=255) agagCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGAc < 1:1865159/71‑1 (MQ=255) aCTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtg > 1:2765414/1‑60 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCttt < 1:1817628/58‑1 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATgg < 1:1153923/69‑1 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaa < 1:1530816/71‑1 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaa < 1:1464835/71‑1 (MQ=255) ttCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa < 1:2279313/44‑1 (MQ=255) cATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgat > 1:2582243/1‑58 (MQ=255) cATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAg > 1:2909059/1‑71 (MQ=255) aTTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCtt > 1:2502901/1‑53 (MQ=255) tACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCa > 1:894166/1‑71 (MQ=255) aCCGGGAAAGTGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCAt > 1:440737/1‑71 (MQ=255) aCCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtga > 1:2353999/1‑53 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:474005/51‑1 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:2429994/51‑1 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:613953/51‑1 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:1401705/51‑1 (MQ=255) gggAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCgg > 1:574689/1‑71 (MQ=255) aaaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgt > 1:3586125/1‑71 (MQ=255) aaaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgt < 1:607385/71‑1 (MQ=255) aaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga < 1:4073854/49‑1 (MQ=255) aaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgtg < 1:3087684/71‑1 (MQ=255) tCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTc < 1:514749/71‑1 (MQ=255) tCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTc < 1:1530638/71‑1 (MQ=255) cAATGGGGATGGCGATGCGCGCCAAAACGCCAATc < 1:1203702/35‑1 (MQ=255) cAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGt > 1:813670/1‑55 (MQ=255) aaTTGGCATGGCGATGCGTGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa > 1:4360123/1‑71 (MQ=255) aaTTGGCATGGCGATGCGTGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa > 1:2965587/1‑71 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaa < 1:2307800/50‑1 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTc < 1:4142023/69‑1 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCg < 1:3850860/70‑1 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa > 1:206015/1‑71 (MQ=255) aTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaaa < 1:1116836/50‑1 (MQ=255) tGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa < 1:276059/69‑1 (MQ=255) tGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGAtt > 1:989430/1‑71 (MQ=255) ggggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATc > 1:4258512/1‑55 (MQ=255) gtgATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgtg > 1:3784698/3‑60 (MQ=255) gggATGGCGATGCGCTCCAAAACGCCAATCTTTGATGACGATg > 1:1468003/1‑43 (MQ=255) gggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATg > 1:518541/1‑43 (MQ=255) gggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATg > 1:2746529/1‑43 (MQ=255) gggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaaaa > 1:1916827/1‑48 (MQ=255) gggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCAt > 1:4183670/1‑53 (MQ=255) gggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTgg > 1:3047458/1‑71 (MQ=255) ggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGc < 1:3953917/71‑1 (MQ=255) ggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGc < 1:1575724/71‑1 (MQ=255) aTGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGa < 1:3769980/38‑1 (MQ=255) aTGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGa < 1:2103711/38‑1 (MQ=255) aTGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGa < 1:3416957/38‑1 (MQ=255) aTGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGa < 1:1343716/38‑1 (MQ=255) tGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAc < 1:2580262/71‑1 (MQ=255) tGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAc < 1:4093609/71‑1 (MQ=255) tGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAc < 1:1821899/71‑1 (MQ=255) ggCGATGCGCGCCAAAACGCCAATCTTTGATGACTATGGAAAAGTCATCGGCGTGGTGTCGATTGGCt > 1:163573/1‑68 (MQ=255) ggCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGTAAAAGTCATCGGCGTGGTGTCGa < 1:2665441/62‑1 (MQ=255) gCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCt < 1:1090680/71‑1 (MQ=255) cGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTg > 1:3216652/1‑71 (MQ=255) gATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTgg > 1:2566168/1‑71 (MQ=255) aTGCGCGCCAAAACGCCAATCTTTGATGACGATGGaaaa < 1:3159771/39‑1 (MQ=255) aTGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGAtt < 1:3136071/60‑1 (MQ=255) | AACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGG > W3110S.gb/651828‑651962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |