| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 687,170 | A→T | 6.2% | intergenic (‑79/+91) | gltJ ← / ← gltI | glutamate and aspartate transporter subunit/glutamate and aspartate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 687,170 | 0 | A | T | 6.2% | 170.4 / 2.4 | 64 | intergenic (‑79/+91) | gltJ/gltI | glutamate and aspartate transporter subunit/glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (29/31); new base T (2/2); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATT > W3110S.gb/687107‑687240 | aaaaGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGa > 1:103201/1‑71 (MQ=255) aaaGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGAt > 1:228597/1‑71 (MQ=255) aaGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATg > 1:3354401/1‑71 (MQ=255) agGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATgggg < 1:1576697/71‑1 (MQ=255) agcagcGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAAc > 1:1503561/1‑69 (MQ=255) gcagcGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCg < 1:1796803/59‑1 (MQ=255) cagcGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGAc > 1:2531893/1‑70 (MQ=255) gcGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCTGGCCCGATGGGGAACGACCac < 1:2531230/71‑2 (MQ=255) gcGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCTGGCCCGATGGGGAACGACCac < 1:949146/71‑2 (MQ=255) gcGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCAt > 1:1877445/1‑71 (MQ=255) gTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCa > 1:3655835/1‑48 (MQ=255) gTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCa > 1:4281272/1‑48 (MQ=255) tAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGAt < 1:3619265/54‑1 (MQ=255) tAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGAt < 1:2906179/54‑1 (MQ=255) aaCTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGc > 1:1720072/1‑48 (MQ=255) aaCTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGc > 1:575640/1‑71 (MQ=255) aaCTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGc > 1:745113/1‑71 (MQ=255) aaCTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGc > 1:750284/1‑71 (MQ=255) aaCTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGc > 1:1194425/1‑71 (MQ=255) aCTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGc < 1:2759222/70‑1 (MQ=255) tGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATg < 1:2749002/51‑1 (MQ=255) gCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCTGGCCCGATGGGGAACGAcc > 1:327284/1‑60 (MQ=255) gCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCTGGCCCGATGGGGAACGAcc > 1:3833825/1‑60 (MQ=255) cTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGta > 1:2863883/1‑69 (MQ=255) cTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGta < 1:1551706/69‑1 (MQ=255) cTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCCta < 1:1040096/69‑1 (MQ=255) cTACCCTCGAAGATTGTTACCCAGCGTAGTGCGGTTTTCAGGCCCGATGGGGAACGa > 1:1514708/1‑57 (MQ=255) cccTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAg < 1:12646/69‑1 (MQ=255) ccTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCt < 1:418769/71‑1 (MQ=255) cTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTg < 1:1832841/71‑1 (MQ=255) tCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATc < 1:3779481/56‑1 (MQ=255) gAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCa > 1:1775985/1‑52 (MQ=255) gAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCa > 1:1221366/1‑52 (MQ=255) gAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGta > 1:2333567/1‑61 (MQ=255) gAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGta > 1:152262/1‑61 (MQ=255) gAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGta > 1:768710/1‑61 (MQ=255) aTTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGta < 1:2794598/57‑1 (MQ=255) tGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGtata > 1:664324/1‑57 (MQ=255) tGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTa > 1:907639/1‑71 (MQ=255) tGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTa > 1:2803456/1‑71 (MQ=255) tGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTa > 1:2569910/1‑71 (MQ=255) gTTACCCAGCGTATTGCGGTTTTCAGGCCCGATgg > 1:1548980/1‑35 (MQ=255) gTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAg > 1:467212/1‑49 (MQ=255) aCCCAACGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGt < 1:369055/70‑1 (MQ=255) cccAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTa < 1:763261/70‑1 (MQ=255) cAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAg < 1:3709045/51‑1 (MQ=255) cAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACa < 1:937197/71‑1 (MQ=255) gCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCa > 1:4141244/1‑40 (MQ=255) gCGTATTGCGGTTTTCAGGCCCGATGGGGAACAACCAt < 1:1366658/38‑1 (MQ=255) cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACaa > 1:1459319/1‑69 (MQ=255) cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATc < 1:2322652/71‑1 (MQ=255) cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATc < 1:3903085/71‑1 (MQ=255) gTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAg < 1:1641248/47‑1 (MQ=255) gTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACg < 1:1312225/62‑1 (MQ=255) tATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCga < 1:3950260/71‑1 (MQ=255) tATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCga < 1:455120/71‑1 (MQ=255) ttGCGGTTTTCAGGCCCGATGGGGAACGACCATCa > 1:1943174/1‑35 (MQ=255) ttGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGt < 1:3235609/45‑1 (MQ=255) ttGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCt < 1:3532516/47‑1 (MQ=255) ttGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACa < 1:3917752/64‑1 (MQ=255) ttGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACa < 1:297025/64‑1 (MQ=255) ttGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCgag < 1:1443027/70‑1 (MQ=255) tttCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCga < 1:3616321/62‑1 (MQ=255) ttCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTgg > 1:477608/1‑70 (MQ=255) ttCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTgg > 1:630314/1‑70 (MQ=255) ttCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTgg < 1:1485852/70‑1 (MQ=255) aGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAAtt < 1:1046038/71‑1 (MQ=255) | AAAAGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCAGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATT > W3110S.gb/687107‑687240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |