| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 715,548 | C→T | 10.6% | L523L (CTC→CTT) | pgm → | phosphoglucomutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 715,548 | 0 | C | T | 10.6% | 131.1 / 5.4 | 47 | L523L (CTC→CTT) | pgm | phosphoglucomutase |
| Reads supporting (aligned to +/- strand): ref base C (20/22); new base T (3/2); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
ACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCG > W3110S.gb/715478‑715617 | acAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTc > 1:3839635/1‑71 (MQ=255) cAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCg < 1:1476613/71‑1 (MQ=255) cAACGGCTGGTTAGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTACCTCg > 1:1714840/1‑71 (MQ=255) aaCGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCgg > 1:2513081/1‑71 (MQ=255) ggCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTga < 1:2845324/71‑1 (MQ=255) gTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAAc < 1:3254596/71‑1 (MQ=255) tCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACAt > 1:693215/1‑71 (MQ=255) gccgcGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATc < 1:649408/70‑1 (MQ=255) gtccgtcAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTTGGTgaagaa > 1:2257527/1‑59 (MQ=255) gtccgtcAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTTGGTGAAGAAc > 1:879184/1‑60 (MQ=255) gtccgtcAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTTGGTGAAGAAc > 1:773951/1‑60 (MQ=255) cgtcAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCaa > 1:1181709/1‑64 (MQ=255) cgtcAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGttt < 1:735544/71‑1 (MQ=255) tcAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTgaaga > 1:1380627/1‑53 (MQ=255) aGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGc > 1:3677334/1‑58 (MQ=255) gCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAg < 1:2536892/59‑1 (MQ=255) cACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAg < 1:2514422/58‑1 (MQ=255) cACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGagaaag < 1:2845571/71‑1 (MQ=255) gAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGc > 1:1503143/1‑71 (MQ=255) gAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGc > 1:381912/1‑71 (MQ=255) cGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTg < 1:775384/71‑1 (MQ=255) gCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGtt > 1:3300976/1‑69 (MQ=255) gCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTga < 1:2566064/71‑1 (MQ=255) tataAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGc < 1:853754/42‑1 (MQ=255) tataAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGAtt < 1:1686217/51‑1 (MQ=255) tataAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTga < 1:2998939/68‑1 (MQ=255) taAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTga > 1:1232361/1‑51 (MQ=255) taAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTg > 1:1448628/1‑71 (MQ=255) taAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTg > 1:3382355/1‑71 (MQ=255) aGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGt > 1:1978296/1‑70 (MQ=255) gATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGt < 1:4139303/69‑1 (MQ=255) aTCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAg < 1:1001119/39‑1 (MQ=255) cTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGc > 1:3415268/1‑70 (MQ=255) tACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTga > 1:3022904/1‑44 (MQ=255) cTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCg < 1:797351/68‑1 (MQ=255) cTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGa < 1:1268294/69‑1 (MQ=255) tGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCa < 1:2872635/35‑1 (MQ=255) tGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCa < 1:3250068/35‑1 (MQ=255) gCGAAAGCTTCCTTGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCg < 1:1604738/50‑1 (MQ=255) gCGAAAGCTTCCTTGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCg < 1:2425450/50‑1 (MQ=255) cGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTc > 1:2287408/1‑71 (MQ=255) gAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCt < 1:1888328/71‑1 (MQ=255) aaaGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGaa > 1:4344204/1‑65 (MQ=255) aaaGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGaa > 1:3533835/1‑65 (MQ=255) aGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGc > 1:398101/1‑60 (MQ=255) aGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGa > 1:1835995/1‑70 (MQ=255) tCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGc < 1:788480/40‑1 (MQ=255) cTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGAt < 1:2791008/47‑1 (MQ=255) cGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTgaga > 1:155858/1‑44 (MQ=255) cGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAAcgcg > 1:2076681/1‑70 (MQ=255) | ACAACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAAAGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGCGAAGTTCTGAAAAACGCG > W3110S.gb/715478‑715617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |