Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 756,245 T→C 5.0% A88A (GCT→GCC sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb756,2450TC 5.0% 214.8 / 2.2 79A88A (GCT→GCCsdhDsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base T (42/33);  new base C (2/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTG  >  W3110S.gb/756175‑756314
                                                                      |                                                                     
tGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCt                                                                       >  1:3685208/1‑71 (MQ=255)
 gTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCtt                                                                      >  1:1365366/1‑71 (MQ=255)
  ttttCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCt                                                                       <  1:3893610/69‑1 (MQ=255)
    ttCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTgc                                                                   >  1:2874685/1‑71 (MQ=255)
    ttCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTgc                                                                   >  1:190138/1‑71 (MQ=255)
         tCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCt                                                               <  1:566257/70‑1 (MQ=255)
         tCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCt                                                               <  1:2160517/70‑1 (MQ=255)
          cTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGa                                                             >  1:652858/1‑71 (MQ=255)
           ttGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCt                                                               <  1:4155087/68‑1 (MQ=255)
             gATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCCTTGCGCCTGAtgc                                                          >  1:1888195/1‑71 (MQ=255)
              aTCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTgc                                                                   <  1:1530863/61‑1 (MQ=255)
                ccATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                        <  1:3809116/70‑1 (MQ=255)
                caaTGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGAGCCTGAtgctg                                                        <  1:857587/68‑1 (MQ=255)
                 cATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctgc                                                       >  1:3908354/1‑70 (MQ=255)
                 cATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCa                                                      <  1:2608371/71‑1 (MQ=255)
                   tGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                    <  1:3473099/71‑1 (MQ=255)
                   tGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                    >  1:1106508/1‑71 (MQ=255)
                     ccTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTg                                                  >  1:3522202/1‑71 (MQ=255)
                       tGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCt                                                               <  1:2128099/56‑1 (MQ=255)
                         gATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGt                                                <  1:3690920/69‑1 (MQ=255)
                         gATCGGCATGTGCCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTg                                               <  1:3330013/70‑1 (MQ=255)
                           tCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGAt                                             >  1:1273602/1‑70 (MQ=255)
                            cGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGAtt                                            >  1:3016352/1‑70 (MQ=255)
                                   tgGCAGGTGTTGACCGACTACGTTAAACCGCTGGCttt                                                                     >  1:494858/1‑38 (MQ=255)
                                   tgGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTg                                     >  1:3543004/1‑70 (MQ=255)
                                   tgGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTg                                     >  1:1033271/1‑70 (MQ=255)
                                   tgGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTg                                     >  1:1061685/1‑70 (MQ=255)
                                    ggCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTcct                                       >  1:1709600/1‑65 (MQ=255)
                                      cAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACt                                 >  1:1974278/1‑71 (MQ=255)
                                      cAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACt                                 <  1:4212785/71‑1 (MQ=255)
                                       aGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtg                                <  1:3704310/71‑1 (MQ=255)
                                        ggTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtgg                               >  1:2947051/1‑71 (MQ=255)
                                        ggTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtgg                               <  1:2902714/71‑1 (MQ=255)
                                         gtgtTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtgg                               <  1:3046048/70‑1 (MQ=255)
                                         gtgtTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACt                                 <  1:2887589/68‑1 (MQ=255)
                                         gtgtTGACCGACTACGTTAAACCGCTGGCCTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtggt                              >  1:1478359/1‑71 (MQ=255)
                                           gtTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtggtgg                            >  1:3954443/1‑71 (MQ=255)
                                            ttGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTg                                               <  1:4103071/51‑1 (MQ=255)
                                            ttGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTg                                               <  1:855433/51‑1 (MQ=255)
                                             tGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGt                                          >  1:1280747/1‑55 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGa                                              >  1:592313/1‑50 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtggtgg                            <  1:3494796/68‑1 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtggt                              >  1:3019203/1‑66 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTggtggt                           >  1:2576339/1‑69 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGtt                          <  1:1815240/70‑1 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGtt                          >  1:2286132/1‑70 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGtt                          >  1:2248/1‑70 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCCTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtg                                <  1:4299007/64‑1 (MQ=255)
                                              gACCGACTACGTTAAACCGCTGGCCTTGCGCCTGATGCTGCAACTGTGATTGTCGTTGCACtg                                <  1:4163920/63‑1 (MQ=255)
                                                  gACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGt                     <  1:4278101/71‑1 (MQ=255)
                                                   aCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                    <  1:2203019/39‑1 (MQ=255)
                                                   aCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                    <  1:2386835/39‑1 (MQ=255)
                                                   aCTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGttt                         <  1:27850/66‑1 (MQ=255)
                                                    cTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACg                      >  1:562043/1‑68 (MQ=255)
                                                       cGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAtt                 >  1:3258959/1‑70 (MQ=255)
                                                       cGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAtt                 >  1:1709185/1‑70 (MQ=255)
                                                       cGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAtt                 >  1:494782/1‑70 (MQ=255)
                                                       cGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTAGTGGTTTACGTGAtt                 <  1:1426096/70‑1 (MQ=255)
                                                        gTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGt                                          <  1:1735839/44‑1 (MQ=255)
                                                          tAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:835814/1‑71 (MQ=255)
                                                          tAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:2910708/1‑71 (MQ=255)
                                                          tAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:1558150/1‑71 (MQ=255)
                                                          tAAACCGCTGGCTTTGCGCCTGATGATGCTACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:4060715/1‑71 (MQ=255)
                                                           aaaCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:942112/1‑70 (MQ=255)
                                                           aaaCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:4005274/1‑70 (MQ=255)
                                                           aaaCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:2334483/1‑70 (MQ=255)
                                                           aaaCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             >  1:238300/1‑70 (MQ=255)
                                                            aaCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGa           >  1:2086608/1‑71 (MQ=255)
                                                                 cTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCg       >  1:3268508/1‑70 (MQ=255)
                                                                 cTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGt      >  1:840818/1‑71 (MQ=255)
                                                                  tGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACt                                 >  1:2495245/1‑43 (MQ=255)
                                                                  tGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACt                                 >  1:1152488/1‑43 (MQ=255)
                                                                  tGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACt                                 >  1:3283654/1‑43 (MQ=255)
                                                                  tGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGtt     >  1:2745693/1‑71 (MQ=255)
                                                                  tGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGtt     >  1:1472121/1‑71 (MQ=255)
                                                                   ggCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATg             <  1:344103/62‑1 (MQ=255)
                                                                    gCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTTGTGGTTTACGTGATTTATGGATTCGTtgt   <  1:2776078/71‑1 (MQ=255)
                                                                    gCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTa               <  1:2604509/59‑1 (MQ=255)
                                                                    gCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGa           >  1:2377882/1‑63 (MQ=255)
                                                                    gCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtg    <  1:1460860/70‑1 (MQ=255)
                                                                     cTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAtt                 <  1:3514406/56‑1 (MQ=255)
                                                                     cTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtg  <  1:3704478/71‑1 (MQ=255)
                                                                     cTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtg  <  1:2503151/71‑1 (MQ=255)
                                                                      |                                                                     
TGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTG  >  W3110S.gb/756175‑756314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: