Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 849,377 T→C 6.4% intergenic (‑44/+255) dps ← / ← rhtA Fe‑binding and storage protein/threonine and homoserine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb849,3770TC 6.4% 164.7 / 2.9 62intergenic (‑44/+255)dps/rhtAFe‑binding and storage protein/threonine and homoserine efflux system
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TAACTAATTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAACAAGC  >  W3110S.gb/849312‑849445
                                                                 |                                                                    
tAACTAATTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAg                                                                  >  1:4423576/1‑70 (MQ=255)
   cTAATTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTaa                                                                   <  1:3237494/66‑1 (MQ=255)
     aaTTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAg                                                             >  1:1726522/1‑70 (MQ=255)
      aTTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGc                                                            <  1:1661386/70‑1 (MQ=255)
      aTTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGc                                                            <  1:3947776/70‑1 (MQ=255)
        ttAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCAcc                                                         >  1:365238/1‑71 (MQ=255)
            cGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCt                                                     <  1:4035671/71‑1 (MQ=255)
              gTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTAt                                                   <  1:2976690/71‑1 (MQ=255)
                aCTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGACTAAGt                                                                 >  1:2003729/1‑55 (MQ=255)
                 cTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAGCGAGATTAAGTATAGCa                                                           >  1:1704703/1‑60 (MQ=255)
                 cTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCa                                                           >  1:3569129/1‑60 (MQ=255)
                 cTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCa                                                           >  1:1497944/1‑60 (MQ=255)
                  tCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGAt                                                                      <  1:2317012/48‑1 (MQ=255)
                  tCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAg                                                                  <  1:4260629/52‑1 (MQ=255)
                   cATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGtt                                              >  1:2067736/1‑71 (MQ=255)
                    aTAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTc                                             >  1:536985/1‑71 (MQ=255)
                    aTAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTc                                             >  1:708986/1‑71 (MQ=255)
                    aTAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTc                                             >  1:2050480/1‑71 (MQ=255)
                     tAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAg                                                                  <  1:1902185/49‑1 (MQ=255)
                     tAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAg                                                                  <  1:54500/49‑1 (MQ=255)
                     tAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGta                                                                <  1:2475819/51‑1 (MQ=255)
                     tAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGta                                                                <  1:1434782/51‑1 (MQ=255)
                      aaTTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTa                                                    >  1:919336/1‑62 (MQ=255)
                       aTTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGta                                                                >  1:1542826/1‑49 (MQ=255)
                          tCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTa                                        <  1:2792427/70‑1 (MQ=255)
                            atatCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTAtgtg                                                >  1:806916/1‑60 (MQ=255)
                            atatCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTAtgtg                                                >  1:2038019/1‑60 (MQ=255)
                            atatCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTAtt                                      <  1:652635/70‑1 (MQ=255)
                            atatCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTc                                     <  1:2827029/71‑1 (MQ=255)
                                 ctctTGATGTTATTTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTAtgt                                                 >  1:4030484/1‑54 (MQ=255)
                                 ctctTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGc                                 <  1:736750/70‑1 (MQ=255)
                                 ctctTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCt                                <  1:507202/71‑1 (MQ=255)
                                 ctctTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCt                                <  1:1719254/71‑1 (MQ=255)
                                   ctTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTgg                                  <  1:1646405/67‑1 (MQ=255)
                                    ttGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGc                                          <  1:3802541/58‑1 (MQ=255)
                                     tGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTc                            >  1:829199/1‑71 (MQ=255)
                                      gATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTc                            <  1:3333592/70‑1 (MQ=255)
                                       aTGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCt                          <  1:2650919/71‑1 (MQ=255)
                                          ttATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGc                                                      <  1:712119/40‑1 (MQ=255)
                                            aTGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATca                      <  1:34734/70‑1 (MQ=255)
                                               tCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACt                  <  1:3160547/71‑1 (MQ=255)
                                               tCCCAGTAATTAACGAGACTAAGTATAGCACCAGCTATGTGTTCCGCTATTCt                                    >  1:4410017/1‑53 (MQ=255)
                                                   aGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTTTTCCTATCACACTaa                >  1:805707/1‑69 (MQ=255)
                                                    gTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCt                                         <  1:2946986/43‑1 (MQ=255)
                                                       aTTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTgg          >  1:330027/1‑71 (MQ=255)
                                                       aTTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTgg          <  1:3164643/71‑1 (MQ=255)
                                                       aTTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTgg          >  1:2392631/1‑71 (MQ=255)
                                                       aTTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTgg          >  1:1965976/1‑71 (MQ=255)
                                                       aTTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTgg          >  1:1898641/1‑71 (MQ=255)
                                                       aTTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTg           <  1:723646/70‑1 (MQ=255)
                                                        ttAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAg             <  1:3587158/67‑1 (MQ=255)
                                                         tAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCt                                    >  1:2806955/1‑43 (MQ=255)
                                                         tAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGt         >  1:4269906/1‑70 (MQ=255)
                                                           aCGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAAc      <  1:2583993/71‑1 (MQ=255)
                                                           aCGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAAc      <  1:1578683/71‑1 (MQ=255)
                                                            cGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATc                       >  1:779545/1‑53 (MQ=255)
                                                            cGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATa              >  1:1173864/1‑62 (MQ=255)
                                                            cGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAAc      >  1:1648297/1‑70 (MQ=255)
                                                            cGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAAc      >  1:2432988/1‑70 (MQ=255)
                                                            cGAGACTAAGTATAGCACCAGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAACa     <  1:944347/71‑1 (MQ=255)
                                                            cGAGACTAAGTATAGCACCAGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAACa     <  1:4317900/71‑1 (MQ=255)
                                                             gagaTTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAAc      >  1:3343351/1‑69 (MQ=255)
                                                               gaTTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAACAAGc  <  1:1384652/71‑1 (MQ=255)
                                                                 |                                                                    
TAACTAATTTAGCGGTACTCATAATTTCATATCCTCTTGATGTTATGTCCCAGTAATTAACGAGATTAAGTATAGCACCGGCTATGTGTTCCGCTATTCTGGCTGTTCCTATCACACTAATAGTGGTAACAAGC  >  W3110S.gb/849312‑849445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: