Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,017,036	C→T	6.1%	A562A (GCG→GCA)	ycbZ ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,017,036	0	C	T	6.1%	256.5 / 4.8	82	A562A (GCG→GCA)	ycbZ	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (51/26);  new base T (3/2);  total (54/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAAT  >  W3110S.gb/1016967‑1017105                                                                      |                                                                      cGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTgcg                                                                      >  1:2741162/1‑71 (MQ=255)  ggAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTgcgc                                                                     <  1:2800/71‑1 (MQ=255)  ggAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGAGATGCTTgcgc                                                                     <  1:2400635/71‑1 (MQ=255)     aTAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGa                                                                   <  1:4111285/70‑1 (MQ=255)     aTAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGAt                                                                  <  1:865587/71‑1 (MQ=255)        aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCTATGCTTGCGCGATACg                                                               <  1:2011660/71‑1 (MQ=255)        aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATAc                                                                >  1:3146380/1‑70 (MQ=255)         aCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGt                                                              <  1:2790644/71‑1 (MQ=255)         aCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGt                                                              <  1:2250471/71‑1 (MQ=255)         aCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGt                                                              <  1:2237145/71‑1 (MQ=255)           cAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTc                                                            >  1:1030034/1‑71 (MQ=255)           cAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTc                                                            >  1:2932305/1‑71 (MQ=255)           cAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTc                                                            >  1:2485842/1‑71 (MQ=255)           cAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTc                                                            >  1:1040038/1‑71 (MQ=255)            aGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTTCGCGATACGTTcc                                                           >  1:3918442/1‑71 (MQ=255)            aGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTcc                                                           >  1:2412125/1‑71 (MQ=255)            aGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTcc                                                           >  1:4328471/1‑71 (MQ=255)            aGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTcc                                                           >  1:3583846/1‑71 (MQ=255)             gTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCt                                                          >  1:1360974/1‑71 (MQ=255)             gTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTAGCGCGATACGTTCCt                                                          >  1:3186277/1‑71 (MQ=255)              ttCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                                         >  1:4259452/1‑71 (MQ=255)              ttCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                                         <  1:891762/71‑1 (MQ=255)               tCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                                         >  1:3680598/1‑70 (MQ=255)               tCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                                         >  1:4384099/1‑70 (MQ=255)                cAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGa                                                       >  1:3182390/1‑71 (MQ=255)                    cAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGt                                                   <  1:3567082/71‑1 (MQ=255)                     aaCGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGt                                                   >  1:4334279/1‑70 (MQ=255)                        gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTg                                                >  1:4267683/1‑70 (MQ=255)                        gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTg                                                >  1:4056876/1‑70 (MQ=255)                         tAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCa                                              >  1:442410/1‑71 (MQ=255)                           aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             >  1:3660841/1‑70 (MQ=255)                           aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             >  1:2432415/1‑70 (MQ=255)                           aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             >  1:2462514/1‑70 (MQ=255)                           aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             <  1:1877185/70‑1 (MQ=255)                           aTGGCCATGGAAAACGGTGACGTCCTTCATGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             >  1:615531/1‑70 (MQ=255)                            tGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                                         <  1:3985926/57‑1 (MQ=255)                              gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTc                                                            >  1:1366791/1‑52 (MQ=255)                               ccATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcg                                        <  1:2528047/71‑1 (MQ=255)                               ccATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcg                                        <  1:2909769/71‑1 (MQ=255)                                 aTGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtc                                      <  1:3091591/71‑1 (MQ=255)                                  tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCAt                                             <  1:4382651/63‑1 (MQ=255)                                  tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCAt                                             <  1:3762292/63‑1 (MQ=255)                                  tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATgg                                                    <  1:1715160/56‑1 (MQ=255)                                  tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             >  1:970456/1‑63 (MQ=255)                                  tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCAt                                             >  1:1751156/1‑63 (MQ=255)                                  tGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAg                                          >  1:1828113/1‑66 (MQ=255)                                   ggAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGttt                                                 <  1:1645833/58‑1 (MQ=255)                                    gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGtt                                                  >  1:423170/1‑56 (MQ=255)                                    gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGt                                                   >  1:4324653/1‑55 (MQ=255)                                    gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGtt                                   >  1:1107574/1‑71 (MQ=255)                                     aaaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGttt                                  <  1:378835/71‑1 (MQ=255)                                     aaaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGttt                                  >  1:1148980/1‑71 (MQ=255)                                        aCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGc                               <  1:860305/71‑1 (MQ=255)                                        aCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGc                               <  1:1041379/71‑1 (MQ=255)                                         cGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGtt                                                             >  1:1609556/1‑40 (MQ=255)                                           gTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGttt                                                 <  1:4104813/50‑1 (MQ=255)                                             gACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATgg                                                    >  1:1324324/1‑45 (MQ=255)                                              aCGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCa                                              >  1:694469/1‑50 (MQ=255)                                                 tccttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCAt                      <  1:3302616/71‑1 (MQ=255)                                                  ccttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                                     >  1:4371954/1‑55 (MQ=255)                                                  ccttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                                     >  1:4377058/1‑55 (MQ=255)                                                  ccttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                                     >  1:1962738/1‑55 (MQ=255)                                                  ccttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATc                     >  1:638685/1‑71 (MQ=255)                                                   cttcctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGttt                                  >  1:1740697/1‑57 (MQ=255)                                                        tGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCacac               >  1:1012002/1‑71 (MQ=255)                                                         gTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                                    >  1:2522586/1‑49 (MQ=255)                                                          ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTg                                                >  1:820300/1‑36 (MQ=255)                                                          ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                                    <  1:2381274/48‑1 (MQ=255)                                                          ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                                    <  1:751154/48‑1 (MQ=255)                                                          ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                                     <  1:2136820/47‑1 (MQ=255)                                                          ttGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCacac               >  1:3097585/1‑69 (MQ=255)                                                           tGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCa                       >  1:960597/1‑60 (MQ=255)                                                           tGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCacac               >  1:1324828/1‑68 (MQ=255)                                                           tGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCacac               >  1:965997/1‑68 (MQ=255)                                                            gCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                                     <  1:4341518/45‑1 (MQ=255)                                                               aTGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGAttt        <  1:3935112/71‑1 (MQ=255)                                                                tGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGtt                                   >  1:1710057/1‑43 (MQ=255)                                                                tGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTta       <  1:3065002/71‑1 (MQ=255)                                                                 gCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcca                             >  1:3298046/1‑47 (MQ=255)                                                                 gCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTta       >  1:2762524/1‑70 (MQ=255)                                                                  cTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGttt                                  >  1:2347325/1‑42 (MQ=255)                                                                  cTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCca                  >  1:261298/1‑58 (MQ=255)                                                                  cTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCca                  >  1:809210/1‑58 (MQ=255)                                                                  cTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaa      >  1:757702/1‑70 (MQ=255)                                                                  cTTGCGAGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaa      >  1:3449178/1‑70 (MQ=255)                                                                     gcgcGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaataa   >  1:562995/1‑70 (MQ=255)                                                                     gcgcGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaataa   >  1:2321414/1‑70 (MQ=255)                                                                     gcgcGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaata    >  1:2812857/1‑69 (MQ=255)                                                                      cgcgATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaataat  <  1:1574586/70‑1 (MQ=255)                                                                      cgcgATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaataat  <  1:1630197/70‑1 (MQ=255)                                                                      cgcgATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTtaataat  <  1:3072166/70‑1 (MQ=255)                                                                      |                                                                      CGGAATAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCACACCAGATTTAATAAT  >  W3110S.gb/1016967‑1017105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑