Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 90.8% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT90.8% 1065.3 / 78.7 401N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (25/12);  new base T (176/188);  total (201/200)
Fisher's exact test for biased strand distribution p-value = 3.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 403 total aligned reads displayed.

TGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGC  >  W3110S.gb/70364‑70504
                                                                      |                                                                      
  aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      <  1:1021883/71‑1 (MQ=255)
  aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:3519918/1‑71 (MQ=255)
  aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGAAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGc                                                                      >  1:4217481/1‑71 (MQ=255)
    tggtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGcc                                                                     >  1:1074916/1‑70 (MQ=255)
    tggtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGcc                                                                     >  1:1708535/1‑70 (MQ=255)
    tggtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGcc                                                                     >  1:3626872/1‑70 (MQ=255)
      gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCAt                                                                  <  1:3421303/71‑1 (MQ=255)
       tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATc                                                                 <  1:442139/71‑1 (MQ=255)
       tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATc                                                                 <  1:282539/71‑1 (MQ=255)
         ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtg                                                               <  1:2037890/71‑1 (MQ=255)
         ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCt                                                                <  1:1003428/70‑1 (MQ=255)
             tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATc                                                                 <  1:3710425/65‑1 (MQ=255)
               tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCg                                                         >  1:1451182/1‑71 (MQ=255)
               tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCg                                                         >  1:2945388/1‑71 (MQ=255)
               tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGc                                                          <  1:3390750/70‑1 (MQ=255)
                gAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCgg                                                        >  1:1281214/1‑71 (MQ=255)
                 aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       <  1:2455624/71‑1 (MQ=255)
                 aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       >  1:3039422/1‑71 (MQ=255)
                 aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       <  1:3761469/71‑1 (MQ=255)
                 aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       >  1:1964295/1‑71 (MQ=255)
                 aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       >  1:924016/1‑71 (MQ=255)
                 aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCgg                                                        >  1:1136451/1‑70 (MQ=255)
                  aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                             >  1:2437729/1‑64 (MQ=255)
                  aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCATCTGGTGGCGGGtt                                                     >  1:3967789/1‑71 (MQ=255)
                    aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:3252688/71‑1 (MQ=255)
                    aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:581414/71‑1 (MQ=255)
                    aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                    <  1:1157157/71‑1 (MQ=255)
                    aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGtt                                                     >  1:332987/1‑70 (MQ=255)
                          gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGt                                                      >  1:2551795/1‑63 (MQ=255)
                           cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCAt                                                                  <  1:3455843/50‑1 (MQ=255)
                           cTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                              >  1:2617210/1‑70 (MQ=255)
                            tGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                             <  1:1374900/54‑1 (MQ=255)
                              aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                             <  1:2306458/68‑1 (MQ=255)
                              aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          <  1:554792/71‑1 (MQ=255)
                              aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          <  1:1176250/71‑1 (MQ=255)
                              aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          <  1:822949/71‑1 (MQ=255)
                              aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCAGAtt                                          >  1:876327/1‑71 (MQ=255)
                               aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCgg                                                        >  1:3209074/1‑56 (MQ=255)
                               aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:2085906/71‑1 (MQ=255)
                               aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:267180/71‑1 (MQ=255)
                               aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:540901/71‑1 (MQ=255)
                                gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:479901/1‑69 (MQ=255)
                                gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        <  1:1871428/71‑1 (MQ=255)
                                gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        <  1:194743/71‑1 (MQ=255)
                                   cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:4294939/56‑1 (MQ=255)
                                   cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                     >  1:79331/1‑55 (MQ=255)
                                    aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                    <  1:4154405/71‑1 (MQ=255)
                                     aaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                             <  1:1439668/61‑1 (MQ=255)
                                      aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGccc                                                                    <  1:627956/37‑1 (MQ=255)
                                      aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                             >  1:3123356/1‑44 (MQ=255)
                                      aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCgg                                                        >  1:4076681/1‑49 (MQ=255)
                                      aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTa                                                   <  1:393483/54‑1 (MQ=255)
                                      aaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  >  1:725273/1‑71 (MQ=255)
                                      aaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         >  1:3170809/1‑64 (MQ=255)
                                       attaTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 >  1:759592/4‑71 (MQ=255)
                                       ataaTCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 >  1:3586278/4‑71 (MQ=255)
                                       aTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 >  1:2905737/1‑71 (MQ=255)
                                       aTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGTGGCGGGTTTAACGCCGATTGAGGCCAACg                                >  1:1900896/1‑71 (MQ=255)
                                        tGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        >  1:278619/1‑63 (MQ=255)
                                          aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    >  1:1459458/1‑49 (MQ=255)
                                          aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                              >  1:2845098/1‑71 (MQ=255)
                                           tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                             <  1:139671/71‑1 (MQ=255)
                                           tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                             <  1:1238659/71‑1 (MQ=255)
                                            ccccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                             <  1:2734909/70‑1 (MQ=255)
                                               ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:1332716/44‑1 (MQ=255)
                                               ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCg                                             <  1:4056537/51‑1 (MQ=255)
                                               ctgctgCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGa                                            >  1:3697134/1‑52 (MQ=255)
                                                tgctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                                  >  1:1618330/1‑45 (MQ=255)
                                                  ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTTGa                      >  1:3883150/1‑71 (MQ=255)
                                                  ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCg                       <  1:1767107/70‑1 (MQ=255)
                                                  ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                      >  1:4271515/1‑71 (MQ=255)
                                                   tgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGATTAACGc                                               <  1:434212/45‑1 (MQ=255)
                                                     ccGGGATTCTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtt                    <  1:2264897/70‑1 (MQ=255)
                                                     ccGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                         >  1:1118015/1‑65 (MQ=255)
                                                     ccGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttt                   <  1:1502751/71‑1 (MQ=255)
                                                         gATACTCGTTTAATGCCCATATGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt               <  1:1821863/71‑1 (MQ=255)
                                                           tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          <  1:411165/42‑1 (MQ=255)
                                                             cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa              <  1:336123/68‑1 (MQ=255)
                                                             cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:2396444/70‑1 (MQ=255)
                                                             cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            >  1:2222723/1‑70 (MQ=255)
                                                              tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAgg                                      <  1:612554/43‑1 (MQ=255)
                                                              tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                    >  1:515544/1‑45 (MQ=255)
                                                              tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttt                  <  1:4321004/63‑1 (MQ=255)
                                                              tCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:896885/69‑1 (MQ=255)
                                                               cGTTTAATGCACATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt             >  1:418068/1‑67 (MQ=255)
                                                                gTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc        >  1:3667329/1‑71 (MQ=255)
                                                                gTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc        <  1:1545856/71‑1 (MQ=255)
                                                                 tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                              <  1:2472893/48‑1 (MQ=255)
                                                                 tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTa                            <  1:356918/50‑1 (MQ=255)
                                                                 tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa              <  1:3824341/64‑1 (MQ=255)
                                                                 tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccg       <  1:1667775/71‑1 (MQ=255)
                                                                 tttAACGCCCATCCGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:2886944/66‑1 (MQ=255)
                                                                  ttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgagcga      >  1:1420645/1‑71 (MQ=255)
                                                                   tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttt                 >  1:3909655/1‑59 (MQ=255)
                                                                   tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa              >  1:4042468/1‑62 (MQ=255)
                                                                   tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac     >  1:962805/1‑71 (MQ=255)
                                                                   tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac     >  1:3022474/1‑71 (MQ=255)
                                                                   tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac     >  1:4398215/1‑71 (MQ=255)
                                                                   tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccga      <  1:200711/70‑1 (MQ=255)
                                                                   tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        >  1:2187231/1‑36 (MQ=255)
                                                                      |                                                                      
TGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGC  >  W3110S.gb/70364‑70504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: