Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,040,245 C→T 9.1% S176S (AGC→AGT appB → cytochrome bd‑II oxidase, subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,040,2450CT 9.1% 227.0 / 8.9 77S176S (AGC→AGTappBcytochrome bd‑II oxidase, subunit II
Reads supporting (aligned to +/- strand):  ref base C (33/37);  new base T (3/4);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCATCTGCG  >  W3110S.gb/1040178‑1040313
                                                                   |                                                                    
gcgTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCtt                                                                   <  1:3131650/71‑1 (MQ=255)
gcgTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCtt                                                                   <  1:929491/71‑1 (MQ=255)
gcgTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCtt                                                                   <  1:2199646/71‑1 (MQ=255)
 cgTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTg                                                                  <  1:3564185/71‑1 (MQ=255)
  gTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGcc                                                                     >  1:1272228/1‑67 (MQ=255)
  gTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTgg                                                                 <  1:1184367/71‑1 (MQ=255)
  gTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTgg                                                                 >  1:2496415/1‑71 (MQ=255)
    ggAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGc                                                                      >  1:2122951/1‑64 (MQ=255)
     gAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGa                                                               <  1:1027793/70‑1 (MQ=255)
     gAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGAt                                                              <  1:2517468/71‑1 (MQ=255)
     gAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGAt                                                              <  1:3497937/71‑1 (MQ=255)
     gAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGAt                                                              >  1:2224654/1‑71 (MQ=255)
      aGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGAt                                                              <  1:2905514/70‑1 (MQ=255)
         aTCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGt                                                           >  1:1745079/1‑70 (MQ=255)
         aTCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGt                                                           >  1:821199/1‑70 (MQ=255)
         aTCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGt                                                           >  1:4347905/1‑70 (MQ=255)
          tctcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGa                                                         >  1:1642302/1‑71 (MQ=255)
          tctcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGa                                                         >  1:1664581/1‑71 (MQ=255)
          tctcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGa                                                         >  1:170127/1‑71 (MQ=255)
          tctcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGa                                                         >  1:4346771/1‑71 (MQ=255)
            tcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAtt                                                       >  1:4332976/1‑71 (MQ=255)
            tcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAtt                                                       >  1:3690924/1‑71 (MQ=255)
            tcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAtt                                                       >  1:2423850/1‑71 (MQ=255)
            tcGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAtt                                                       >  1:1893781/1‑71 (MQ=255)
              ggCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAttt                                                      <  1:597941/70‑1 (MQ=255)
              ggCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAtt                                                       <  1:3832748/69‑1 (MQ=255)
              ggCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAtt                                                       <  1:1499536/69‑1 (MQ=255)
              ggCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGAt                                                        <  1:1668846/68‑1 (MQ=255)
                cAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGcc                                                                     <  1:680712/53‑1 (MQ=255)
                cAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGc                                                   <  1:2728578/71‑1 (MQ=255)
                 aGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCa                                                  <  1:287270/71‑1 (MQ=255)
                  gCTTCTGGCATCTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCaa                                                 <  1:1599675/71‑1 (MQ=255)
                   cTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCaa                                                 <  1:4139604/70‑1 (MQ=255)
                    ttCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGc                                                   <  1:1358162/67‑1 (MQ=255)
                     tCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCtt                                                                   <  1:3300038/50‑1 (MQ=255)
                     tCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTggg                                                                >  1:395168/1‑53 (MQ=255)
                     tCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTggg                                                                >  1:430087/1‑53 (MQ=255)
                     tCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTggg                                                                >  1:2133211/1‑53 (MQ=255)
                     tCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGt                                              >  1:1080131/1‑71 (MQ=255)
                        ggCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGa                                                               >  1:677333/1‑51 (MQ=255)
                        ggCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGa                                                               >  1:4414665/1‑51 (MQ=255)
                         gCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTg                                             <  1:1496377/68‑1 (MQ=255)
                         gCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCg                                          >  1:3055530/1‑71 (MQ=255)
                           aaCTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTc                                        <  1:97977/71‑1 (MQ=255)
                            aCTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAg                                                <  1:2257875/62‑1 (MQ=255)
                             ctgctgACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTg                                      >  1:4044975/1‑71 (MQ=255)
                              tgctgACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTg                                      >  1:2079908/1‑70 (MQ=255)
                               gctgACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCaa                                                 >  1:47590/1‑58 (MQ=255)
                                   aCGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGTGATTTTGCAAGGTGGCGTCTg                                      <  1:3672754/65‑1 (MQ=255)
                                   aCGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGTGATTTTGCAAGGTGGCGTCTg                                      <  1:4298862/65‑1 (MQ=255)
                                   aCGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGTGATTTTGCAAGGTGGCGTCTg                                      <  1:4293642/65‑1 (MQ=255)
                                   aCGCCATTCCCTTTATTGTGTGGATTGCTCAGTCTTGGGATGGGGATTTTGCAAGGTGGCGTCTg                                      <  1:136167/65‑1 (MQ=255)
                                   aCGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTc                                        >  1:914646/1‑63 (MQ=255)
                                     gCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAAc                              >  1:645536/1‑71 (MQ=255)
                                      ccATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAg                                                <  1:624489/52‑1 (MQ=255)
                                      ccATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAg                                                <  1:2723012/52‑1 (MQ=255)
                                       cATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACt                             >  1:3152314/1‑70 (MQ=255)
                                         ttCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGc                                                   <  1:4168545/46‑1 (MQ=255)
                                         ttCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGc                                                   <  1:4415568/46‑1 (MQ=255)
                                         ttCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGaa                          <  1:3773919/71‑1 (MQ=255)
                                           cccTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAg                                                >  1:859030/1‑47 (MQ=255)
                                             cTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACt                      <  1:615529/71‑1 (MQ=255)
                                              tttATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTAc                                 <  1:663397/59‑1 (MQ=255)
                                                tATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGtt                   >  1:2289234/1‑71 (MQ=255)
                                                tATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGtt                   >  1:2800743/1‑71 (MQ=255)
                                                tATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGt                    <  1:3358427/70‑1 (MQ=255)
                                                tATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGt                    <  1:2214075/70‑1 (MQ=255)
                                                    gtgCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGgtg               >  1:4402351/1‑71 (MQ=255)
                                                       cGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGtgtg             <  1:914916/70‑1 (MQ=255)
                                                        ggATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTg                     <  1:3090440/61‑1 (MQ=255)
                                                         gATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTTAAAACTGt                    <  1:4059664/61‑1 (MQ=255)
                                                          aTTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGt                    <  1:3213875/60‑1 (MQ=255)
                                                           ttGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCa        <  1:3926798/71‑1 (MQ=255)
                                                           ttGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTATTGGTGTGATTCa        >  1:1404801/1‑71 (MQ=255)
                                                            tGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCAt       >  1:2553480/1‑71 (MQ=255)
                                                            tGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCAt       >  1:1268200/1‑71 (MQ=255)
                                                              cTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGa            >  1:927539/1‑64 (MQ=255)
                                                              cTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCATCt     <  1:4382728/71‑1 (MQ=255)
                                                                 aGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTg                  >  1:697814/1‑55 (MQ=255)
                                                                 aGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCATCTGCg  >  1:14465/1‑71 (MQ=255)
                                                                   |                                                                    
GCGTGGAGTATCTCGGCAGCTTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTGATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCATCTGCG  >  W3110S.gb/1040178‑1040313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: