Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,056,856 T→C 7.2% Y58Y (TAT→TAC torT → periplasmic sensory protein associated with the TorRS two‑component regulatory system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,056,8560TC 7.2% 148.2 / 3.2 57Y58Y (TAT→TACtorTperiplasmic sensory protein associated with the TorRS two‑component regulatory system
Reads supporting (aligned to +/- strand):  ref base T (31/21);  new base C (2/2);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CCTCTACGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTAAAAGTGCTGG  >  W3110S.gb/1056786‑1056923
                                                                      |                                                                   
ccTCTACGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCatat                                                                     >  1:3800199/1‑71 (MQ=255)
    tACGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGt                                                                 >  1:1908343/1‑71 (MQ=255)
    tACGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGt                                                                 >  1:1597254/1‑71 (MQ=255)
    tACGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGt                                                                 >  1:1878303/1‑71 (MQ=255)
     aCGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGt                                                                 >  1:826842/1‑70 (MQ=255)
     aCGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGt                                                                 >  1:2672041/1‑70 (MQ=255)
     aCGCCGATTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGt                                                                 >  1:567179/1‑70 (MQ=255)
      cgccgcTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTa                                                               >  1:527819/1‑71 (MQ=255)
         cgcTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCg                                                            >  1:365178/1‑71 (MQ=255)
                aGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACt                                                     <  1:490865/71‑1 (MQ=255)
                aGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACt                                                     <  1:2732328/71‑1 (MQ=255)
                           cATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCag                                          <  1:758775/71‑1 (MQ=255)
                            aTGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGtt                                                                <  1:1679683/48‑1 (MQ=255)
                            aTGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGtt                                                                <  1:4106002/48‑1 (MQ=255)
                             tGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATAATGGTTATCGTTGAACt                                                     <  1:2012237/58‑1 (MQ=255)
                               gAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGtt                                                          >  1:2809799/1‑51 (MQ=255)
                               gAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAAc                                                      >  1:3143908/1‑55 (MQ=255)
                               gAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCaggagg                                      >  1:1666864/1‑71 (MQ=255)
                               gAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCag                                          >  1:2838686/1‑67 (MQ=255)
                                aaaCTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGgc                                     >  1:4248507/1‑71 (MQ=255)
                                   cTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCg                                                            <  1:2478412/45‑1 (MQ=255)
                                    tgtgCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGCAGgctgct                                 >  1:3908782/1‑71 (MQ=255)
                                    tgtgCGCGCTTTATCCCAGCATGAAAGATTCATATTGGTTATCGTTg                                                         <  1:3864961/47‑1 (MQ=255)
                                     gtgCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTc                                >  1:3531068/1‑71 (MQ=255)
                                      tgCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATgg                                                 <  1:3315723/53‑1 (MQ=255)
                                      tgCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGgctgct                                 <  1:277673/69‑1 (MQ=255)
                                       gcgcgcTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTAt                                                   >  1:3992706/1‑50 (MQ=255)
                                        cgcgcTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTcgcc                             >  1:3482187/1‑71 (MQ=255)
                                         gcgcTTTATCCCAGCCTGAAAGATTCTTATTGGTTATCGTTGAACt                                                     <  1:3309743/46‑1 (MQ=255)
                                         gcgcTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTc                                <  1:3108559/67‑1 (MQ=255)
                                           gcTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCa                                           <  1:2702095/54‑1 (MQ=255)
                                           gcTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTcgccgc                           >  1:2881151/1‑70 (MQ=255)
                                             tttATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTAc                        >  1:666980/1‑71 (MQ=255)
                                               tATCCCAGCCTGAAAGATTCATATTGGTTATCGtt                                                          >  1:3067334/1‑35 (MQ=255)
                                               tATCCCAGCCTGAAAGATTCATATTGGTTATCGtt                                                          >  1:1089555/1‑35 (MQ=255)
                                               tATCCCAGCCTGAAAGATTCATATTGGTTATCGtt                                                          >  1:3243576/1‑35 (MQ=255)
                                               tATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACgg                      >  1:1563449/1‑71 (MQ=255)
                                               tATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACgg                      >  1:3012305/1‑71 (MQ=255)
                                                 tCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACgg                      <  1:3194515/69‑1 (MQ=255)
                                                 tCCCAGCCTGAAAGATTCATACTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGgt                     <  1:926141/70‑1 (MQ=255)
                                                 tCCCAGCCTGAAAGATTCATACTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGgt                     <  1:1308639/70‑1 (MQ=255)
                                                   ccAGCCTGAAAGATTCATACTGGTTATCGTTGAACTATGGTATGCa                                           >  1:2140287/1‑46 (MQ=255)
                                                   ccAGCCTGAAAGATTCATACTGGTTATCGTTGAACTATGGTATGCa                                           >  1:3817312/1‑46 (MQ=255)
                                                     aGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGa                >  1:532437/1‑71 (MQ=255)
                                                       ccTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGgt                     <  1:619775/64‑1 (MQ=255)
                                                        cTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGgt                     <  1:624574/63‑1 (MQ=255)
                                                           aaaGATTCATATTGGTTATCGTTGAACTATGGTAt                                              <  1:2832306/35‑1 (MQ=255)
                                                           aaaGATTCATATTGGTTATCGTTGAACTATGGTATg                                             <  1:3455311/36‑1 (MQ=255)
                                                           aaaGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTaa           <  1:2712014/70‑1 (MQ=255)
                                                           aaaGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTaa           <  1:3797721/70‑1 (MQ=255)
                                                           aaaGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTaa           >  1:885737/1‑70 (MQ=255)
                                                            aaGATTCATATTGGTTATCGTTGAACTATGGTATGCaggagg                                      >  1:1998191/1‑42 (MQ=255)
                                                              gATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTaa           <  1:3098013/67‑1 (MQ=255)
                                                               aTTCATATTGGTTATCGTTGAACTATGGTATGCagga                                        >  1:2999999/1‑37 (MQ=255)
                                                               aTTCATATTGGTTATCGTTGAACTATGGTATGCagga                                        >  1:3494815/1‑37 (MQ=255)
                                                               aTTCATATTGGTTATCGTTGAACTATGGTATGCagga                                        >  1:2232336/1‑37 (MQ=255)
                                                                ttCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTAAAAGTGc     <  1:1399136/71‑1 (MQ=255)
                                                                 tCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTaa           >  1:3153997/1‑64 (MQ=255)
                                                                   atatTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTAAAAGTGCTgg  <  1:928399/71‑1 (MQ=255)
                                                                   atatTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTAAAAGTGCTg   <  1:3721140/70‑1 (MQ=255)
                                                                   atatTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTAAAAGTGCTg   <  1:1600737/70‑1 (MQ=255)
                                                                      |                                                                   
CCTCTACGCCGCTTAAAGCCAAACGCGCATGGAAACTGTGCGCGCTTTATCCCAGCCTGAAAGATTCATATTGGTTATCGTTGAACTATGGTATGCAGGAGGCTGCTCGCCGCTACGGTGTGGATTTAAAAGTGCTGG  >  W3110S.gb/1056786‑1056923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: