Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,069,131 | G→C | 10.4% | V377V (GTC→GTG) | ycdG ← | predicted transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,069,131 | 0 | G | C | 10.4% | 124.7 / 4.8 | 48 | V377V (GTC→GTG) | ycdG | predicted transporter |
Reads supporting (aligned to +/- strand): ref base G (25/18); new base C (3/2); total (28/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCAC > W3110S.gb/1069067‑1069201 | aaTCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACgg > 1:1893628/1‑71 (MQ=255) tCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtt > 1:2668994/1‑71 (MQ=255) aCCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtttt > 1:3023398/1‑71 (MQ=255) cgcCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAcc < 1:1833788/71‑1 (MQ=255) gcCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAccc < 1:575493/71‑1 (MQ=255) cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtt < 1:3438100/57‑1 (MQ=255) cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATcc > 1:657116/1‑71 (MQ=255) cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATcc > 1:1789618/1‑71 (MQ=255) cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc > 1:137610/1‑71 (MQ=255) cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc > 1:3366555/1‑71 (MQ=255) cACTAAGGTTACTGCGACCATAATAAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc > 1:2100800/1‑71 (MQ=255) aCTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGacac > 1:2590254/1‑52 (MQ=255) aCTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGacac > 1:1658985/1‑52 (MQ=255) cTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTa > 1:2742197/1‑60 (MQ=255) aaGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgc > 1:51302/1‑71 (MQ=255) aGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGa > 1:351232/1‑45 (MQ=255) aGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgcg > 1:2970588/1‑71 (MQ=255) ttACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCt < 1:4179661/71‑1 (MQ=255) aCTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATc < 1:4402048/60‑1 (MQ=255) cGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAcc > 1:4307948/1‑50 (MQ=255) cGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAcc > 1:381732/1‑50 (MQ=255) aTAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgcg < 1:2223808/57‑1 (MQ=255) tAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTg < 1:304306/40‑1 (MQ=255) tAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGc < 1:86548/67‑1 (MQ=255) tCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGc > 1:2235671/1‑71 (MQ=255) aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt > 1:2421305/1‑47 (MQ=255) aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt > 1:2278702/1‑47 (MQ=255) aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGccc > 1:114900/1‑71 (MQ=255) aaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCg < 1:2387618/71‑1 (MQ=255) tACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCatat > 1:748229/1‑40 (MQ=255) tACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCatat > 1:3631160/1‑40 (MQ=255) aCCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAaca > 1:217226/1‑71 (MQ=255) gTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACacaa > 1:480058/1‑71 (MQ=255) ttCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt < 1:2744980/38‑1 (MQ=255) cTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACAcaacaa > 1:3972649/1‑71 (MQ=255) gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt < 1:3936537/70‑1 (MQ=255) gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt < 1:794514/70‑1 (MQ=255) gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAt < 1:1763708/69‑1 (MQ=255) cTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGa < 1:2094497/71‑1 (MQ=255) ttAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCa < 1:51056/47‑1 (MQ=255) ttAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAacac < 1:3534475/61‑1 (MQ=255) aaaTCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt < 1:3668888/66‑1 (MQ=255) aaaTCCACACGGTTTTGTACCCAGATCCGTGCGCCTGCGAc < 1:2363383/41‑1 (MQ=37) aaaTCCACACGGTTTTGTACCCAGATCCGTGCGCCTGCGAc < 1:506064/41‑1 (MQ=37) aaTCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCaa > 1:1505103/1‑45 (MQ=255) tCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACacaac < 1:4005326/59‑1 (MQ=255) cGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac > 1:4092741/1‑53 (MQ=255) cGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac > 1:2806879/1‑53 (MQ=255) gACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGcaccac > 1:2234550/1‑71 (MQ=255) | AATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCAC > W3110S.gb/1069067‑1069201 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |