Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,069,131 G→C 10.4% V377V (GTC→GTG ycdG ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,069,1310GC10.4% 124.7 / 4.8 48V377V (GTC→GTGycdGpredicted transporter
Reads supporting (aligned to +/- strand):  ref base G (25/18);  new base C (3/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCAC  >  W3110S.gb/1069067‑1069201
                                                                |                                                                      
aaTCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACgg                                                                  >  1:1893628/1‑71 (MQ=255)
  tCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtt                                                                >  1:2668994/1‑71 (MQ=255)
    aCCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtttt                                                              >  1:3023398/1‑71 (MQ=255)
         cgcCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAcc                                                         <  1:1833788/71‑1 (MQ=255)
          gcCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAccc                                                        <  1:575493/71‑1 (MQ=255)
                cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGtt                                                                <  1:3438100/57‑1 (MQ=255)
                cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATcc                                                  >  1:657116/1‑71 (MQ=255)
                cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATcc                                                  >  1:1789618/1‑71 (MQ=255)
                cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc                                                  >  1:137610/1‑71 (MQ=255)
                cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc                                                  >  1:3366555/1‑71 (MQ=255)
                cACTAAGGTTACTGCGACCATAATAAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc                                                  >  1:2100800/1‑71 (MQ=255)
                 aCTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGacac                                                                    >  1:2590254/1‑52 (MQ=255)
                 aCTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGacac                                                                    >  1:1658985/1‑52 (MQ=255)
                  cTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTa                                                           >  1:2742197/1‑60 (MQ=255)
                    aaGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgc                                              >  1:51302/1‑71 (MQ=255)
                     aGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGa                                                                       >  1:351232/1‑45 (MQ=255)
                     aGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgcg                                             >  1:2970588/1‑71 (MQ=255)
                        ttACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCt                                          <  1:4179661/71‑1 (MQ=255)
                          aCTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATc                                                   <  1:4402048/60‑1 (MQ=255)
                              cGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAcc                                                         >  1:4307948/1‑50 (MQ=255)
                              cGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTAcc                                                         >  1:381732/1‑50 (MQ=255)
                                   aTAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgcg                                             <  1:2223808/57‑1 (MQ=255)
                                    tAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTg                                                             <  1:304306/40‑1 (MQ=255)
                                    tAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGc                                  <  1:86548/67‑1 (MQ=255)
                                       tCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGc                           >  1:2235671/1‑71 (MQ=255)
                                         aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt                                                 >  1:2421305/1‑47 (MQ=255)
                                         aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt                                                 >  1:2278702/1‑47 (MQ=255)
                                         aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGccc                         >  1:114900/1‑71 (MQ=255)
                                          aaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCg                        <  1:2387618/71‑1 (MQ=255)
                                             tACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCatat                                                    >  1:748229/1‑40 (MQ=255)
                                             tACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCatat                                                    >  1:3631160/1‑40 (MQ=255)
                                              aCCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAaca                    >  1:217226/1‑71 (MQ=255)
                                                 gTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACacaa                 >  1:480058/1‑71 (MQ=255)
                                                  ttCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt                                                 <  1:2744980/38‑1 (MQ=255)
                                                    cTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACAcaacaa              >  1:3972649/1‑71 (MQ=255)
                                                       gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt            <  1:3936537/70‑1 (MQ=255)
                                                       gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt            <  1:794514/70‑1 (MQ=255)
                                                       gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAt             <  1:1763708/69‑1 (MQ=255)
                                                        cTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGa          <  1:2094497/71‑1 (MQ=255)
                                                         ttAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCa                                 <  1:51056/47‑1 (MQ=255)
                                                         ttAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAacac                   <  1:3534475/61‑1 (MQ=255)
                                                           aaaTCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt            <  1:3668888/66‑1 (MQ=255)
                                                           aaaTCCACACGGTTTTGTACCCAGATCCGTGCGCCTGCGAc                                     <  1:2363383/41‑1 (MQ=37)
                                                           aaaTCCACACGGTTTTGTACCCAGATCCGTGCGCCTGCGAc                                     <  1:506064/41‑1 (MQ=37)
                                                            aaTCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCaa                                >  1:1505103/1‑45 (MQ=255)
                                                              tCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACacaac                <  1:4005326/59‑1 (MQ=255)
                                                               cGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac                     >  1:4092741/1‑53 (MQ=255)
                                                               cGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac                     >  1:2806879/1‑53 (MQ=255)
                                                                gACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGcaccac  >  1:2234550/1‑71 (MQ=255)
                                                                |                                                                      
AATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCAC  >  W3110S.gb/1069067‑1069201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: