Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,069,149 T→G 11.4% I371I (ATA→ATC ycdG ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,069,1490TG11.4% 89.0 / 9.6 44I371I (ATA→ATCycdGpredicted transporter
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base G (3/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGCCGGAA  >  W3110S.gb/1069083‑1069219
                                                                  |                                                                      
cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATcc                                                                    >  1:657116/1‑71 (MQ=255)
cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATcc                                                                    >  1:1789618/1‑71 (MQ=255)
cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc                                                                    >  1:137610/1‑71 (MQ=255)
cACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc                                                                    >  1:3366555/1‑71 (MQ=255)
cACTAAGGTTACTGCGACCATAATAAAATTACCGTTCTGGCTTAAATCCACACGGTTTTGTACCCAGATcc                                                                    >  1:2100800/1‑71 (MQ=255)
    aaGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgc                                                                >  1:51302/1‑71 (MQ=255)
     aGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgcg                                                               >  1:2970588/1‑71 (MQ=255)
        ttACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCt                                                            <  1:4179661/71‑1 (MQ=255)
          aCTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATc                                                                     <  1:4402048/60‑1 (MQ=255)
                   aTAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTgcg                                                               <  1:2223808/57‑1 (MQ=255)
                    tAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGc                                                    <  1:86548/67‑1 (MQ=255)
                       tCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGc                                             >  1:2235671/1‑71 (MQ=255)
                         aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt                                                                   >  1:2278702/1‑47 (MQ=255)
                         aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt                                                                   >  1:2421305/1‑47 (MQ=255)
                         aaaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGccc                                           >  1:114900/1‑71 (MQ=255)
                          aaTTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCg                                          <  1:2387618/71‑1 (MQ=255)
                             tACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCatat                                                                      >  1:3631160/1‑40 (MQ=255)
                             tACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCatat                                                                      >  1:748229/1‑40 (MQ=255)
                              aCCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAaca                                      >  1:217226/1‑71 (MQ=255)
                                 gTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACacaa                                   >  1:480058/1‑71 (MQ=255)
                                  ttCTGGCTTAAATCGACACGGTTTTGTACCCATATCCt                                                                   <  1:2744980/38‑1 (MQ=255)
                                    cTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACAcaacaa                                >  1:3972649/1‑71 (MQ=255)
                                       gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt                              <  1:3936537/70‑1 (MQ=255)
                                       gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt                              <  1:794514/70‑1 (MQ=255)
                                       gCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAt                               <  1:1763708/69‑1 (MQ=255)
                                        cTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGa                            <  1:2094497/71‑1 (MQ=255)
                                         ttAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCa                                                   <  1:51056/47‑1 (MQ=255)
                                         ttAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAacac                                     <  1:3534475/61‑1 (MQ=255)
                                           aaaTCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt                              <  1:3668888/66‑1 (MQ=255)
                                           aaaTCCACACGGTTTTGTACCCAGATCCGTGCGCCTGCGAc                                                       <  1:506064/41‑1 (MQ=37)
                                           aaaTCCACACGGTTTTGTACCCAGATCCGTGCGCCTGCGAc                                                       <  1:2363383/41‑1 (MQ=37)
                                            aaTCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCaa                                                  >  1:1505103/1‑45 (MQ=255)
                                              tCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACacaac                                  <  1:4005326/59‑1 (MQ=255)
                                               cGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac                                       >  1:2806879/1‑53 (MQ=255)
                                               cGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAac                                       >  1:4092741/1‑53 (MQ=255)
                                                gACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGcaccac                    >  1:2234550/1‑71 (MQ=255)
                                                   acGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATga                         >  1:3414973/1‑62 (MQ=255)
                                                   acGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGcacc                      >  1:3569244/1‑66 (MQ=255)
                                                   acGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCaa                 >  1:312525/1‑71 (MQ=255)
                                                    cGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCaa                 >  1:3958218/1‑70 (MQ=255)
                                                      gTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGaa                                        >  1:4404698/1‑45 (MQ=255)
                                                              cccATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGcc      >  1:3403540/1‑71 (MQ=255)
                                                                cATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGCCgg    <  1:4087341/71‑1 (MQ=255)
                                                                cATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGCCgg    <  1:2857950/71‑1 (MQ=255)
                                                                cATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGCCgg    <  1:22902/71‑1 (MQ=255)
                                                                cATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGCCg     >  1:1862832/1‑70 (MQ=255)
                                                                 atatCCTTGCGCCTGCGACGGCAATCAGCCCGAACAcaacaa                                >  1:990434/1‑42 (MQ=255)
                                                                  tatCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAAtt                              <  1:1713877/43‑1 (MQ=255)
                                                                  tatCCTTGCGCCTGCGACGGCAACCAGCCCGAACACAACAATTGATGCACCACCAATAACGGCGGCCGGaa  <  1:2353845/71‑1 (MQ=255)
                                                                  |                                                                      
CACTAAGGTTACTGCGACCATAATCAAATTACCGTTCTGGCTTAAATCGACACGGTTTTGTACCCATATCCTTGCGCCTGCGACGGCAATCAGCCCGAACACAACAATTGATGCACCACCAATAACCGCGGCCGGAA  >  W3110S.gb/1069083‑1069219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: