| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,326,354 | G→A | 6.9% | P181P (CCG→CCA) | yciO → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,326,354 | 0 | G | A | 6.9% | 175.2 / 2.4 | 58 | P181P (CCG→CCA) | yciO | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (30/24); new base A (2/2); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGC > W3110S.gb/1326291‑1326421 | tAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACgg > 1:1713332/1‑71 (MQ=255) aaaGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACgg < 1:3244087/70‑1 (MQ=255) aaGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGa > 1:1551500/1‑63 (MQ=255) aTCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAtt < 1:191377/71‑1 (MQ=255) aTCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAtt < 1:3333981/71‑1 (MQ=255) tCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAtt < 1:1804778/70‑1 (MQ=255) cGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGa > 1:1426579/1‑71 (MQ=255) aaaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACaa > 1:3527438/1‑54 (MQ=255) aaaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTAcc < 1:2018746/71‑1 (MQ=255) aaaaaCAGGTAGATTTGATTATTCACGGCGGATATCTCGGGCAGAAACCGACAACGGTTATTGATCTTAcc < 1:779749/71‑1 (MQ=255) aaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTAcc > 1:2128220/1‑70 (MQ=255) aaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCg < 1:3374297/71‑1 (MQ=255) aaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCg < 1:3720123/71‑1 (MQ=255) aaaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCg < 1:3065664/71‑1 (MQ=255) aaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCgat < 1:3694715/71‑1 (MQ=255) aaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCgat < 1:2020029/71‑1 (MQ=255) aCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCgat > 1:3393133/1‑70 (MQ=255) cAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCgatga < 1:3541087/71‑1 (MQ=255) ggTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGa > 1:2653564/1‑44 (MQ=255) gATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGcc > 1:3205344/1‑71 (MQ=255) aTTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTg < 1:995121/51‑1 (MQ=255) aTTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGcc < 1:605027/70‑1 (MQ=255) aTTTGATTATTCACGGCGGATATCTCGGGCAGAAACCGACAACGGTTATTg < 1:3225601/51‑1 (MQ=255) tttGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTa < 1:4114224/56‑1 (MQ=255) tttGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCgg > 1:1406834/1‑71 (MQ=255) tttGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCgg > 1:695915/1‑71 (MQ=255) tttGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCg > 1:3710994/1‑70 (MQ=255) attattCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCgt < 1:3189288/71‑1 (MQ=255) ttattCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCgatga < 1:2872410/58‑1 (MQ=255) ttattCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCgtg > 1:2230375/1‑71 (MQ=255) attCACGGCGGCTATCTCGGGCAGAAACCGACAACg > 1:4356484/1‑36 (MQ=255) attCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAtt > 1:948028/1‑42 (MQ=255) aCGGCGGCTATCTCGGGCAGAAACCAACAACGGTTATTGATCTCACCga > 1:1057407/1‑49 (MQ=38) aCGGCGGCTATCTCGGGCAGAAACCAACAACGGTTATTGATCTCACCga > 1:1059750/1‑49 (MQ=38) ggcggcTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTg > 1:1172427/1‑70 (MQ=255) ggcggcTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGa < 1:3453700/71‑1 (MQ=255) gcggcTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGaa < 1:1032621/71‑1 (MQ=255) aTCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCg > 1:3513695/1‑69 (MQ=255) tctcGGGCAGAAACCGACAACGGTTATTGATCTTACCga > 1:1710476/1‑39 (MQ=255) tctcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAg > 1:2621431/1‑71 (MQ=255) tctcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAg > 1:1461089/1‑71 (MQ=255) ctcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAg > 1:462677/1‑70 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTa > 1:723199/1‑68 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTa > 1:718100/1‑68 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTa > 1:3040184/1‑68 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt > 1:3474913/1‑71 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt > 1:2814724/1‑71 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt > 1:613959/1‑71 (MQ=255) tcGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt > 1:581173/1‑71 (MQ=255) gggCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt < 1:101887/69‑1 (MQ=255) cAGAAACCGACAACGGTTATTGATCTTAGCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgt > 1:2885557/1‑71 (MQ=255) aGAAACCGACAACGGTTATTGATCTTACCGATTATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgtg < 1:528351/71‑1 (MQ=255) aGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgtg < 1:1429119/71‑1 (MQ=255) aGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgt > 1:175690/1‑70 (MQ=255) aGAAACCAACAACGGTTATTGATCTCACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgtg < 1:2584153/71‑1 (MQ=255) aGAAACCAACAACGGTTATTGATCTCACCGATGATACGCCGGTCGTGGTGCGTCAAGGCGTAGGTGAtgtg < 1:1654380/71‑1 (MQ=39) gAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGa < 1:2102813/54‑1 (MQ=255) aCCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGc > 1:2712368/1‑71 (MQ=255) | TAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGC > W3110S.gb/1326291‑1326421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |