| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,491,906 | C→T | 6.2% | pseudogene (522/1001 nt) pseudogene (522/753 nt) |
gapC ← gapC ← |
ECK1409:JW5906+JW1413:b4493; glyceraldehyde‑3‑phosphate dehydrogenase C ECK1409:JW5906:b1417; glyceraldehyde‑3‑phosphate dehydrogenase C, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,491,906 | 0 | C | T | 6.2% | 196.6 / 2.2 | 64 | pseudogene (522/1001 nt) pseudogene (522/753 nt) | gapC gapC | ECK1409:JW5906+JW1413:b4493; glyceraldehyde‑3‑phosphate dehydrogenase C ECK1409:JW5906:b1417; glyceraldehyde‑3‑phosphate dehydrogenase C, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (26/34); new base T (2/2); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
AGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTG > W3110S.gb/1491840‑1491975 | aGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCa < 1:3020423/71‑1 (MQ=255) gCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCAt < 1:2352583/71‑1 (MQ=255) aCGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATc < 1:1993989/70‑1 (MQ=255) cGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGt < 1:2122861/71‑1 (MQ=255) tAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGc > 1:653893/1‑71 (MQ=255) aaaTCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGcc > 1:66777/1‑71 (MQ=255) aaaTCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGcc > 1:2191463/1‑71 (MQ=255) aaaTCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGcc > 1:2311521/1‑71 (MQ=255) aaTCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGcc > 1:4115561/1‑70 (MQ=255) aaTCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGcc < 1:148178/70‑1 (MQ=255) tttACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACtt > 1:2154817/1‑71 (MQ=255) tACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGAc < 1:214179/67‑1 (MQ=255) tACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGAc < 1:3154114/67‑1 (MQ=255) tACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTc < 1:2756686/70‑1 (MQ=255) gggCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGaa < 1:2525411/71‑1 (MQ=255) aTCCACCAGTGACTGGGTGCCGGTATAGGCATGAATGGTTGTCATCGTGCCGACTTCTATCCCGAAACTGt > 1:3289130/1‑71 (MQ=255) aTCCACCAGTGACTGGGTGCCGGTATAGGCATGAATGGTTGTCATCGTGCCGACTTCTATCCCGAAACTGt > 1:348458/1‑71 (MQ=255) aTCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGa < 1:1922260/53‑1 (MQ=255) aTCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGt < 1:2878110/71‑1 (MQ=255) aTCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGt < 1:1295822/71‑1 (MQ=255) ttcaccaGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGt > 1:1144444/3‑70 (MQ=255) tCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGaa < 1:1694800/65‑1 (MQ=255) tCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGaa < 1:3402548/65‑1 (MQ=255) tCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGt > 1:1787662/1‑70 (MQ=255) caccaGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTa > 1:2838255/1‑56 (MQ=255) ccaGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGt < 1:2056982/66‑1 (MQ=255) ccaGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATg > 1:2620194/1‑70 (MQ=255) ccaGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATg > 1:952267/1‑70 (MQ=255) ccaGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGc < 1:311838/71‑1 (MQ=255) caGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCa < 1:655176/71‑1 (MQ=255) aCTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCt < 1:3039623/71‑1 (MQ=255) cTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCtt < 1:426244/71‑1 (MQ=255) tGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCttt > 1:306193/1‑71 (MQ=255) ggTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTgg > 1:1005102/1‑71 (MQ=255) ccggTATAGGCATGAATGGTTGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCAt < 1:129127/68‑1 (MQ=255) ccggTATAGGCATGAATGGTTGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCAt < 1:1419611/68‑1 (MQ=255) ccAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGaaa > 1:4234427/1‑48 (MQ=255) tataGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGc < 1:536840/53‑1 (MQ=255) tataGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGc < 1:3003366/64‑1 (MQ=255) ataGGCATGAATGGTCGTCATCGTGCCGACTTCTATcc > 1:2947524/1‑38 (MQ=255) ataGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTgg > 1:2942451/1‑62 (MQ=255) taGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGa < 1:2934651/40‑1 (MQ=255) taGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCa > 1:2150958/1‑64 (MQ=255) aGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGc < 1:1492960/50‑1 (MQ=255) ggCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGgcgc > 1:2008544/1‑69 (MQ=255) ggCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCaa < 1:743058/71‑1 (MQ=255) ggCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCaa < 1:129809/71‑1 (MQ=255) ggCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCaa < 1:3828355/71‑1 (MQ=255) tGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCttt < 1:4296021/53‑1 (MQ=255) gAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCaa < 1:1619263/66‑1 (MQ=255) gAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCaa < 1:1706493/66‑1 (MQ=255) gAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGAc > 1:1205521/1‑69 (MQ=255) aaTGGTCGTCATCGTGCGGACTTCTATCCCGAAACTGTCATGCAAGGCTTTgg > 1:3595276/1‑53 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCaa > 1:2849243/1‑45 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCaa > 1:1161862/1‑45 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTgg > 1:1771588/1‑53 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGt < 1:28668/71‑1 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTATCCAGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGt < 1:4347339/71‑1 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTATCACGAAACTGTCATGCAAgg > 1:3578091/1‑47 (MQ=255) aaTGGTCGTCATCGTGCCGACTTCTAT‑‑CGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGt > 1:1651144/1‑69 (MQ=255) aTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCtt > 1:242085/1‑49 (MQ=255) aTGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGtt < 1:4037042/71‑1 (MQ=255) tGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCa < 1:2025033/62‑1 (MQ=255) tGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTa < 1:3664756/71‑1 (MQ=255) gtcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGt < 1:4257352/67‑1 (MQ=255) gtcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGt < 1:1243853/67‑1 (MQ=255) gtcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGt < 1:1013715/67‑1 (MQ=255) gtcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAg < 1:3555229/70‑1 (MQ=255) gtcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAg > 1:1664486/1‑70 (MQ=255) tcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTgg < 1:237271/48‑1 (MQ=255) tcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCa > 1:1671910/1‑51 (MQ=255) tcgtcATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAgtg > 1:3849996/1‑71 (MQ=255) cgtcATCGTGCCGACTTCTATCCCTAAACTGTCATGCAAGGCTTTGGCCATCGGCTCa < 1:3191122/58‑1 (MQ=255) | AGCACGTAAATCTTTACCACGCGGGCCATCCACCAGTGACTGGGTGCCAGTATAGGCATGAATGGTCGTCATCGTGCCGACTTCTATCCCGAAACTGTCATGCAAGGCTTTGGCCATCGGCGCAAGACAGTTAGTG > W3110S.gb/1491840‑1491975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |