| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,537,075 | G→A | 5.7% | I166I (ATC→ATT) | yddE ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,537,075 | 0 | G | A | 5.7% | 216.9 / 2.1 | 70 | I166I (ATC→ATT) | yddE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (28/38); new base A (2/2); total (30/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
TTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTCAGT > W3110S.gb/1537006‑1537143 | tttGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGa > 1:3637847/1‑71 (MQ=255) aTAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGAtcatca < 1:3251098/70‑1 (MQ=255) aTAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAc > 1:1674079/1‑71 (MQ=255) aTAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCAc > 1:4263682/1‑71 (MQ=255) aGCGGGCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACt < 1:3053336/70‑1 (MQ=255) cGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt < 1:34085/71‑1 (MQ=255) cGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt > 1:3438932/1‑71 (MQ=255) gTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGa < 1:957066/71‑1 (MQ=255) cgcATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc < 1:591080/71‑1 (MQ=255) cATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCt < 1:256205/71‑1 (MQ=255) ttAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgt > 1:2438184/1‑71 (MQ=255) aaGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg > 1:1057100/1‑71 (MQ=255) aGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg < 1:1093456/70‑1 (MQ=255) aGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg < 1:2301941/70‑1 (MQ=255) cAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGc > 1:2918151/1‑70 (MQ=255) cAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGc > 1:3914928/1‑70 (MQ=255) aGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGc > 1:3180935/1‑69 (MQ=255) gCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGa > 1:2126381/1‑52 (MQ=255) gCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAcc > 1:1593241/1‑71 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc > 1:1909836/1‑39 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc > 1:1898221/1‑39 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt > 1:224646/1‑71 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt > 1:4137137/1‑71 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt > 1:1839887/1‑71 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt < 1:2738123/71‑1 (MQ=255) aGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAt > 1:3091824/1‑71 (MQ=255) aGGGCGTCGATATCCACTTCCGACTTCAGTGGAATCATCACTTTTGTGTGTCCTgttgtt > 1:7665/1‑60 (MQ=255) aGGGCGTCGATATCCACTTCCGACTTCAGTGGAATCATCACTTTTGAGTGTCCTgttgtt > 1:2197696/1‑60 (MQ=255) ggCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGa < 1:607406/67‑1 (MQ=255) ggCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg > 1:4416154/1‑71 (MQ=255) gTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCa < 1:2808305/71‑1 (MQ=255) gTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGCTGCCACCTGAATTGGCa < 1:3199841/71‑1 (MQ=255) tCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg < 1:3987626/52‑1 (MQ=255) tCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg < 1:3502946/52‑1 (MQ=255) tCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAtt < 1:264843/66‑1 (MQ=255) tCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAtt < 1:3359334/66‑1 (MQ=255) tCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAAtt < 1:3742761/66‑1 (MQ=255) gATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGc > 1:2786375/1‑71 (MQ=255) atatCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc < 1:493395/71‑1 (MQ=255) atatCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc > 1:381637/1‑71 (MQ=255) tatCCACTTCCGGTTTCAGTGGGATCATCACTTTTg < 1:988886/36‑1 (MQ=255) tatCCACTTCCGGTTTCAGTGGGATCATCACTTTTg < 1:1912554/36‑1 (MQ=255) tatCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg < 1:605970/48‑1 (MQ=255) tatCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg < 1:2312868/48‑1 (MQ=255) tatCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt < 1:1199640/50‑1 (MQ=255) tCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgt < 1:2948879/44‑1 (MQ=255) tCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt < 1:181801/48‑1 (MQ=255) tCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTg < 1:3413045/70‑1 (MQ=255) cACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTgg > 1:3438826/1‑69 (MQ=255) aCTTCCGGTTTCAGTGGGATCATCACTTTTTAGTGTCCTGTTGTTGc > 1:3577606/1‑47 (MQ=255) aCTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGc > 1:1095915/1‑47 (MQ=255) aCTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAg < 1:1966022/71‑1 (MQ=255) aCTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAg < 1:3881708/71‑1 (MQ=255) aCTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAg < 1:3133764/71‑1 (MQ=255) cTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg < 1:4391663/57‑1 (MQ=255) cTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGa < 1:4318297/71‑1 (MQ=255) cTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGT‑CTGTTGTTGCCACCTg < 1:3951328/51‑1 (MQ=255) ttCCGACTTCAGTGGAATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg < 1:2650442/56‑1 (MQ=255) ttCCGACTTCAGTGGAATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTg < 1:853614/56‑1 (MQ=255) tCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc > 1:4147488/1‑47 (MQ=255) tCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc > 1:4299126/1‑47 (MQ=255) tCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTg < 1:1132377/50‑1 (MQ=255) ccGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGcc > 1:2917013/1‑61 (MQ=255) ggTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATc > 1:3696435/1‑71 (MQ=255) gTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt < 1:3598860/35‑1 (MQ=255) ttCAGTGGGATCATCACTTTTGAGTGTTCtgttgt > 1:703925/1‑35 (MQ=255) tCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGc < 1:1021710/59‑1 (MQ=255) aGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTc < 1:1886024/71‑1 (MQ=255) gTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc < 1:486877/37‑1 (MQ=255) gTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTCa < 1:1675572/71‑1 (MQ=255) gggATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGaa > 1:3934068/1‑40 (MQ=255) gggATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTCAg > 1:138674/1‑70 (MQ=255) ggATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTCa > 1:2154242/1‑68 (MQ=255) ggATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTCAGt < 1:512621/70‑1 (MQ=255) | TTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAATTGGCAAGCCTGGCAGAATATCGTCCTCAGT > W3110S.gb/1537006‑1537143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |