| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,764,980 | G→A | 9.6% | V248V (GTC→GTT) | sufB ← | component of SufBCD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,764,980 | 0 | G | A | 9.6% | 147.5 / 5.2 | 52 | V248V (GTC→GTT) | sufB | component of SufBCD complex |
| Reads supporting (aligned to +/- strand): ref base G (28/19); new base A (2/3); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
ACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAA > W3110S.gb/1764912‑1765044 | aCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGAc > 1:2842611/1‑71 (MQ=255) cTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACg > 1:2953861/1‑71 (MQ=255) ccaccaCTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAg > 1:585775/1‑71 (MQ=255) ccacTGCCGCGTGTAACTGATAGCTGTGACGCACCGGAGCGGAACAGCCTTCAATGTAGCTg < 1:907913/62‑1 (MQ=255) ccacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTg < 1:1251590/62‑1 (MQ=255) ccacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTg < 1:1172987/62‑1 (MQ=255) cacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGt > 1:2509358/1‑71 (MQ=255) acTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGAc > 1:2144836/1‑62 (MQ=255) cTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCt < 1:1851546/71‑1 (MQ=255) ccGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtcg > 1:1202434/1‑71 (MQ=255) cgcgTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtcgt > 1:2439457/1‑71 (MQ=255) cgTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtcgtcg > 1:1582235/1‑71 (MQ=255) tAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCtt > 1:340445/1‑62 (MQ=255) tAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGcca > 1:2228102/1‑71 (MQ=255) aCTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTg > 1:1542626/1‑56 (MQ=255) aCTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccac > 1:1502323/1‑70 (MQ=255) aTAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAg < 1:2057048/69‑1 (MQ=255) aTAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAACTGTCTTCGTCGGCCACCAg < 1:3011896/69‑1 (MQ=255) aGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGa > 1:3427909/1‑68 (MQ=255) cTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGt < 1:2299270/71‑1 (MQ=255) cTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGt > 1:455534/1‑71 (MQ=255) cTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTAACGTAGCTGTCTTCGTCGGCCACCAGAATGGt > 1:2603466/1‑71 (MQ=255) cTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTAACGTAGCTGTCTTCGTCGGCCACCAGAATGGt > 1:2833937/1‑71 (MQ=255) tCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTAACGTAGCTGTCTTCGTCGGCCACCAGaa < 1:2047000/64‑1 (MQ=255) tCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTAACGTAGCTGTCTTCGTCGGCCACCAGaa < 1:435843/64‑1 (MQ=255) tCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTAACGTAGCTGTCTTCGTCGGCCACCAGaa < 1:1914306/64‑1 (MQ=255) tCACGCACCGGAGCCGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtc < 1:2357204/50‑1 (MQ=255) cACGCACCGGAGCGGAACACCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAAt < 1:916225/64‑1 (MQ=255) aCGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccacca > 1:508281/1‑59 (MQ=255) aCGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccacca > 1:2065934/1‑59 (MQ=255) gCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTc > 1:3516092/1‑70 (MQ=255) gCACCGGACCGGAACAGCCTTCAATGTAGCTGACTTAGCt > 1:3878983/1‑40 (MQ=38) cACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCaa < 1:3040487/71‑1 (MQ=255) cACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCaa < 1:3045648/71‑1 (MQ=255) aCCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAg > 1:3662243/1‑36 (MQ=255) aCCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCaaa < 1:3118890/71‑1 (MQ=255) ggAGCGGAACAGCCTTCAATGTAGGTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTg > 1:1389173/1‑71 (MQ=255) ggAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtc < 1:2058112/41‑1 (MQ=255) ggAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtc < 1:4365692/41‑1 (MQ=255) ggAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTg > 1:2920349/1‑71 (MQ=255) ggAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTg > 1:3410474/1‑71 (MQ=255) gCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGccc > 1:3501558/1‑71 (MQ=255) gCGGAACAGCCTACAATGTAGCTGACGTACCTGTCTtcgtcg > 1:3930418/1‑42 (MQ=255) ggAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAAt < 1:3837552/51‑1 (MQ=255) aGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGttttt < 1:2632909/71‑1 (MQ=255) ccTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCa < 1:643636/54‑1 (MQ=255) ccTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTc > 1:3516022/1‑71 (MQ=255) cAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGaaa > 1:2548050/1‑39 (MQ=255) aaTGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCaaa > 1:2958807/1‑51 (MQ=255) aTGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGtt < 1:4242146/71‑1 (MQ=255) aTGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGtt < 1:4402788/71‑1 (MQ=255) aTGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGtt < 1:2763415/71‑1 (MQ=255) tGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTa < 1:1385056/71‑1 (MQ=255) tAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTaa < 1:3740327/70‑1 (MQ=255) tGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGc > 1:2583044/1‑61 (MQ=255) | ACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAA > W3110S.gb/1764912‑1765044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |