Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,770,916 G→A 8.1% T43T (ACG→ACA ydiK → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,770,9160GA 8.1% 144.0 / 2.7 49T43T (ACG→ACAydiKpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base A (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGC  >  W3110S.gb/1770848‑1770984
                                                                    |                                                                    
gCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACggt                                                                    <  1:617152/71‑1 (MQ=255)
gCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACgg                                                                     <  1:688763/70‑1 (MQ=255)
gCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACgg                                                                     <  1:2318919/70‑1 (MQ=255)
   atcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACggtggt                                                                 <  1:3072207/71‑1 (MQ=255)
    tcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGtt                                                                <  1:3347816/71‑1 (MQ=255)
     catGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTa                                                               >  1:900150/1‑71 (MQ=255)
     catGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTa                                                               >  1:743279/1‑71 (MQ=255)
      atGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTa                                                               <  1:327188/70‑1 (MQ=255)
           tgtgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGACGGTACGGTGGTTATCGCCa                                                         >  1:4317346/1‑71 (MQ=255)
             tgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCAcc                                                       <  1:2619568/71‑1 (MQ=255)
                   gTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTAt                                                              >  1:1184771/1‑58 (MQ=255)
                   gTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCg                                                 >  1:833224/1‑71 (MQ=255)
                    tCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGTGCCGGTACGGTGGTTATCGCCACCTGGCCgg                                                <  1:3692661/71‑1 (MQ=255)
                    tCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCgg                                                <  1:3626969/71‑1 (MQ=255)
                     cTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCt                                                      >  1:3488582/1‑64 (MQ=255)
                     cTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCgg                                                >  1:3749050/1‑70 (MQ=255)
                        tgGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTAtt                                            >  1:1282260/1‑71 (MQ=255)
                          gATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGTTATTGt                                          <  1:579646/71‑1 (MQ=255)
                           aTTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACggtg                                                                   >  1:846635/1‑45 (MQ=255)
                           aTTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGtt                                         <  1:2918816/71‑1 (MQ=255)
                             tGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTAc                                       >  1:1717059/1‑71 (MQ=255)
                               ttCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTgg                                                    <  1:1435353/56‑1 (MQ=255)
                               ttCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTgg                                                    <  1:1468120/56‑1 (MQ=255)
                                tCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGtt                                    >  1:2160671/1‑71 (MQ=255)
                                 cAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGttt                                   >  1:478229/1‑71 (MQ=255)
                                 cAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGttt                                   >  1:2792486/1‑71 (MQ=255)
                                    cccTTTATTCTCGGCTTTGCATGGGCCGGTACAGTGGTTATCGCCAc                                                        >  1:8331/1‑47 (MQ=255)
                                    cccTTTATTCTCGGCTTTGCATGGGCCGGTACAGTGGTTATCGCCAc                                                        >  1:3087401/1‑47 (MQ=255)
                                       tttATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCaa                               <  1:135335/69‑1 (MQ=255)
                                        ttATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGcc                                                          <  1:846200/41‑1 (MQ=255)
                                        ttATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTa                                              >  1:1315840/1‑53 (MQ=255)
                                         tATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCaaaa                             <  1:417676/69‑1 (MQ=255)
                                          aTTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGt                                     >  1:959019/1‑60 (MQ=255)
                                          aTTCTCGGCTTTGCATGGGCCGGTACGGTCGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCaa                               >  1:4153246/1‑66 (MQ=255)
                                           ttCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGAt                          <  1:3819886/70‑1 (MQ=255)
                                           ttCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATc                         <  1:1892853/71‑1 (MQ=255)
                                              tcGGCTTTGCATGGGCCGGTACGGTTGTTATCGCCa                                                         >  1:2660585/1‑36 (MQ=255)
                                                ggCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATg                      <  1:2308809/69‑1 (MQ=255)
                                                ggCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGtt                    <  1:3292659/71‑1 (MQ=255)
                                                ggCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGt                     <  1:179680/70‑1 (MQ=255)
                                                ggCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGt                     <  1:184781/70‑1 (MQ=255)
                                                ggCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGt                     <  1:2381978/70‑1 (MQ=255)
                                                  cTTTGCATTGGCCGGTACAGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGt                     <  1:285716/68‑1 (MQ=255)
                                                  cTTTGCATGGTCCGGTACAGTGGTTATCGCCAACTGGCCGGTATTGTTACGTTTGCAAAAGATCATGt                     <  1:1716945/68‑1 (MQ=255)
                                                      gCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTa                                              <  1:3196164/39‑1 (MQ=255)
                                                               ggTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGctctct     <  1:805326/71‑1 (MQ=255)
                                                               ggTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGctctct     >  1:1758105/1‑71 (MQ=255)
                                                               ggTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGctctct     >  1:3153828/1‑71 (MQ=255)
                                                                 tACGGTGGTTATCGTCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCa                        >  1:2112796/1‑50 (MQ=255)
                                                                 tACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCa                        >  1:714859/1‑50 (MQ=255)
                                                                 tACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCa                        >  1:2437810/1‑50 (MQ=255)
                                                                 tACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCa                        >  1:2752253/1‑50 (MQ=255)
                                                                  aCGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGc  >  1:634397/1‑71 (MQ=255)
                                                                  aCGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGc  >  1:2832126/1‑71 (MQ=255)
                                                                    |                                                                    
GCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGC  >  W3110S.gb/1770848‑1770984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: